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anti-F8 antibody :: Mouse FACTOR VIII Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS219502
Testing Data
Unit / Price
0.2 mg  /  $430 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

FACTOR VIII (F8), Monoclonal Antibody

★Popular Item★
 Also Known As   

MOUSE ANTI HUMAN FACTOR VIII

 Product Gene Name   

anti-F8 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    134500
 3D Structure    ModBase 3D Structure for P00451
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    RFF-VIIIC/5
 Host    Mouse
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 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide (NaN3)
Preparation
 Immunogen    Affinity purified human Factor VIII
Buffer Solution
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-F8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-F8 antibody

   Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/5 recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/5 recognizes an epitope towards the C-terminus of full-length Factor VIII. It also recognizes the 80kDa and 70kDa cleavage products.
 Applications Tested/Suitable for anti-F8 antibody   

ELISA (EIA), Radioimmunoassays (RIA), Western Blot (WB)

 Application Notes for anti-F8 antibody    ELISA: Minimum Dilution: 1/400; Maximum Dilution: 1/4000
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 Testing Data of anti-F8 antibody    Recombinant Human Factor VIII detected with Mouse anti Human Factor VIII (MBS219440)
anti-F8 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for F8. It may not necessarily be applicable to this product.
 NCBI GI #    4503647
 NCBI GeneID    2157
 NCBI Accession #    NP_000123.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000132.3 [Other Products]
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 UniProt Primary Accession #    P00451 [Other Products]
 UniProt Secondary Accession #    Q14286; Q5HY69 [Other Products]
 UniProt Related Accession #    P00451 [Other Products]
 Molecular Weight    24,641 Da [Similar Products]
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 NCBI Official Full Name    coagulation factor VIII isoform a
 NCBI Official Synonym Full Names    coagulation factor VIII, procoagulant component
 NCBI Official Symbol    F8 [Similar Products]
 NCBI Official Synonym Symbols   
AHF; F8B; F8C; HEMA; FVIII; DXS1253E
[Similar Products]
 NCBI Protein Information    coagulation factor VIII; antihemophilic factor; coagulation factor VIIIc; factor VIII F8B
 UniProt Protein Name    Coagulation factor VIII
 UniProt Synonym Protein Names   
Antihemophilic factor; AHF; Procoagulant componentCleaved into the following 4 chains:Factor VIIIa heavy chain, 200 kDa isoform; Factor VIIIa heavy chain, 92 kDa isoform; Factor VIII B chain; Factor VIIIa light chain
 Protein Family    Factor VIII intron 22 protein
 UniProt Gene Name    F8 [Similar Products]
 UniProt Synonym Gene Names    F8C; AHF [Similar Products]
 UniProt Entry Name    FA8_HUMAN
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 NCBI Summary for F8    This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]
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 UniProt Comments for F8    F8: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Defects in F8 are the cause of hemophilia A (HEMA). A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non- functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: protein binding; copper ion binding; serine-type endopeptidase activity; oxidoreductase activity

Biological Process: platelet activation; platelet degranulation; acute-phase response; blood coagulation; proteolysis; blood coagulation, intrinsic pathway

Disease: Hemophilia A; Factor Viii Deficiency
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 Research Articles on F8    1. Translation of the F8B gene comprising F8 exons 23-26 may be dependent on the position of the premature stop codon and thus contributes to the immune response of truncated FVIII proteins.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-F8 antibodyPathways associated with anti-F8 antibody
 Reference Product  PubMed Publications
 VWF antibody  >61 publications with F8 and VWF
 F10 antibody  >26 publications with F8 and F10
 SERPINE1 antibody  >3 publications with F8 and SERPINE1
 LMAN1 antibody  >3 publications with F8 and LMAN1
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
Diseases associated with anti-F8 antibodyOrgans/Tissues associated with anti-F8 antibody
 Disease Name  Pubmed Publications
 Hemophilia A Antibodies  >534 publications with F8 and Hemophilia A
 Hemorrhage Antibodies  >137 publications with F8 and Hemorrhage
 Nervous System Diseases Antibodies  >108 publications with F8 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >92 publications with F8 and Cardiovascular Diseases
 Thrombosis Antibodies  >75 publications with F8 and Thrombosis
 Brain Diseases Antibodies  >52 publications with F8 and Brain Diseases
 Heart Diseases Antibodies  >30 publications with F8 and Heart Diseases
 Kidney Diseases Antibodies  >27 publications with F8 and Kidney Diseases
 Inflammation Antibodies  >26 publications with F8 and Inflammation
 Venous Thrombosis Antibodies  >26 publications with F8 and Venous Thrombosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >517 publications with F8 and Blood
 Brain Antibodies  >149 publications with F8 and Brain
 Vascular Antibodies  >97 publications with F8 and Vascular
 Muscle Antibodies  >55 publications with F8 and Muscle
 Kidney Antibodies  >54 publications with F8 and Kidney
 Heart Antibodies  >40 publications with F8 and Heart
 Liver Antibodies  >38 publications with F8 and Liver
 Nerve Antibodies  >27 publications with F8 and Nerve
 Lung Antibodies  >26 publications with F8 and Lung
 Skin Antibodies  >24 publications with F8 and Skin
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