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anti-FRAP antibody :: Mouse anti-Human FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2090651
Western Blot (WB)
Unit / Price
0.01 mg  /  $105 +1 FREE 8GB USB
0.02 mg  /  $110 +1 FREE 8GB USB
0.05 mg  /  $145 +1 FREE 8GB USB
0.1 mg  /  $175 +1 FREE 8GB USB
0.2 mg  /  $230 +1 FREE 8GB USB
1 mg  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP), Monoclonal Antibody

 Also Known As   

Monoclonal Antibody to FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP)

 Product Synonym Names    FRAP1; MTOR; FRAP2; MTOR; RAFT1; RAPT1; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target 1
 Product Gene Name   

anti-FRAP antibody

[Similar Products]
 Matching Pairs    Antibody: FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Clone #D4 (MBS2090651)
Antigen: FK506 Binding Protein 12 (MBS2031638)
 Matching Pairs    Unconjugated Antibody: FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Clone #D4 (MBS2090651)
Biotin Conjugated Secondary Antibody: IgG (MBS2086030)
 Matching Pairs    Unconjugated Antibody: FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Clone #D4 (MBS2090651)
FITC Conjugated Secondary Antibody: IgG (MBS2086031)
 Matching Pairs    Unconjugated Antibody: FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Clone #D4 (MBS2090651)
Biotin Conjugated Secondary Antibody: IgG (MBS2086033)
 Matching Pairs    Unconjugated Antibody: FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Clone #D4 (MBS2090651)
FITC Conjugated Secondary Antibody: IgG (MBS2086034)
 Matching Pairs    Unconjugated Antibody: FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Clone #D4 (MBS2090651)
HRP Conjugated Secondary Antibody: IgG (MBS2086035)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    L34075 mRNA
 3D Structure    ModBase 3D Structure for P42345
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    D4
 Host    Mouse
 Species Reactivity    Human
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 Specificity    The antibody is a mouse monoclonal antibody raised against FRAP. It has been selected for its ability to recognize FRAP in immunohistochemical staining and western blotting.
 Purity/Purification    Protein A + Protein G affinity chromatography
 Form/Format    Liquid
 Concentration    1mg/mL (lot specific)
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 Immunogen    Recombinant FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP)
 Buffer Formulation    0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of anti-FRAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-FRAP antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

 Application Notes for anti-FRAP antibody    Western Blot: 0.5-5ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
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 Western Blot (WB) of anti-FRAP antibody    (Figure. Western Blot; Sample: Recombinant FRAP, Human.)
anti-FRAP antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for FRAP. It may not necessarily be applicable to this product.
 NCBI GI #    4826730
 NCBI GeneID    2475
 NCBI Accession #    NP_004949.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004958.3 [Other Products]
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 UniProt Primary Accession #    P42345 [Other Products]
 UniProt Secondary Accession #    Q4LE76; Q5TER1; Q6LE87; Q96QG3; Q9Y4I3 [Other Products]
 UniProt Related Accession #    P42345 [Other Products]
 Molecular Weight    288,892 Da
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 NCBI Official Full Name    serine/threonine-protein kinase mTOR
 NCBI Official Synonym Full Names    mechanistic target of rapamycin kinase
 NCBI Official Symbol    MTOR [Similar Products]
 NCBI Official Synonym Symbols   
SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase mTOR
 UniProt Protein Name    Serine/threonine-protein kinase mTOR
 UniProt Synonym Protein Names   
FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1
 UniProt Gene Name    MTOR [Similar Products]
 UniProt Synonym Gene Names    FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR [Similar Products]
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 NCBI Summary for FRAP    The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
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 UniProt Comments for FRAP    mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).

Protein type: ATYPICAL group; Autophagy; EC 2.7.11.1; FRAP subfamily; Kinase, protein; Motility/polarity/chemotaxis; PIKK family; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cell soma; cytoplasm; cytosol; dendrite; endomembrane system; endoplasmic reticulum membrane; Golgi membrane; lysosomal membrane; lysosome; membrane; mitochondrial outer membrane; nucleoplasm; nucleus; phosphoinositide 3-kinase complex; PML body; TORC2 complex

Molecular Function: ATP binding; identical protein binding; kinase activity; phosphoprotein binding; protein binding; protein domain specific binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; ribosome binding

Biological Process: activation of protein kinase B activity; anoikis; brain development; cardiac muscle cell development; cardiac muscle contraction; cell aging; cell cycle arrest; cellular response to amino acid starvation; cellular response to hypoxia; cellular response to leucine starvation; cellular response to nutrient levels; cellular response to starvation; de novo'pyrimidine base biosynthetic process; DNA repair; energy reserve metabolic process; germ cell development; growth; heart morphogenesis; heart valve morphogenesis; long-term memory; maternal process involved in female pregnancy; mRNA stabilization; multicellular organism growth; negative regulation of autophagy; negative regulation of cell size; negative regulation of cholangiocyte apoptotic process; negative regulation of iodide transmembrane transport; negative regulation of macroautophagy; negative regulation of muscle atrophy; negative regulation of NFAT protein import into nucleus; negative regulation of protein phosphorylation; negative regulation of protein ubiquitination; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of actin filament polymerization; positive regulation of cholangiocyte proliferation; positive regulation of eating behavior; positive regulation of endothelial cell proliferation; positive regulation of epithelial to mesenchymal transition; positive regulation of gene expression; positive regulation of granulosa cell proliferation; positive regulation of keratinocyte migration; positive regulation of lamellipodium assembly; positive regulation of lipid biosynthetic process; positive regulation of myotube differentiation; positive regulation of neuron death; positive regulation of neuron maturation; positive regulation of nitric oxide biosynthetic process; positive regulation of oligodendrocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; positive regulation of sensory perception of pain; positive regulation of skeletal muscle hypertrophy; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; post-embryonic development; protein amino acid phosphorylation; protein autophosphorylation; protein catabolic process; regulation of actin cytoskeleton organization; regulation of brown fat cell differentiation; regulation of carbohydrate utilization; regulation of cell growth; regulation of cell size; regulation of cellular response to heat; regulation of fatty acid beta-oxidation; regulation of glycogen biosynthetic process; regulation of GTPase activity; regulation of macroautophagy; regulation of membrane permeability; regulation of myelination; regulation of osteoclast differentiation; regulation of response to food; response to amino acid; response to cocaine; response to insulin stimulus; response to morphine; response to nutrient; ruffle organization; signal transduction; social behavior; spinal cord development; TOR signaling; TORC1 signaling; visual learning; voluntary musculoskeletal movement; wound healing

Disease: Focal Cortical Dysplasia Of Taylor; Smith-kingsmore Syndrome
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 Research Articles on FRAP    1. Ehrlichia chaffeensis infection activated the phosphatidylinositol 3-kinase (PI3K)-Akt-mTOR (mechanistic target of rapamycin) pathway, and activation was induced by three ehrlichial tandem repeat protein (TRP) effectors, with TRP120 inducing the strongest activation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-FRAP antibodyPathways associated with anti-FRAP antibody
 Reference Product  PubMed Publications
 EIF4EBP1 antibody  >444 publications with FRAP and EIF4EBP1
 AKT1 antibody  >342 publications with FRAP and AKT1
 RICTOR antibody  >243 publications with FRAP and RICTOR
 RHEB antibody  >233 publications with FRAP and RHEB
 STAT3 antibody  >155 publications with FRAP and STAT3
 IRS1 antibody  >117 publications with FRAP and IRS1
 RPTOR antibody  >109 publications with FRAP and RPTOR
 RPS6KA1 antibody  >74 publications with FRAP and RPS6KA1
 AKT1S1 antibody  >21 publications with FRAP and AKT1S1
 TERT antibody  >19 publications with FRAP and TERT
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
Diseases associated with anti-FRAP antibodyOrgans/Tissues associated with anti-FRAP antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4311 publications with FRAP and Neoplasms
 Nervous System Diseases Antibodies  >776 publications with FRAP and Nervous System Diseases
 Adenocarcinoma Antibodies  >772 publications with FRAP and Adenocarcinoma
 Kidney Diseases Antibodies  >635 publications with FRAP and Kidney Diseases
 Breast Neoplasms Antibodies  >520 publications with FRAP and Breast Neoplasms
 Drug Toxicity Antibodies  >435 publications with FRAP and Drug Toxicity
 Cardiovascular Diseases Antibodies  >424 publications with FRAP and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >379 publications with FRAP and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >363 publications with FRAP and Lung Neoplasms
 Hypertrophy Antibodies  >336 publications with FRAP and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1270 publications with FRAP and Blood
 Muscle Antibodies  >1066 publications with FRAP and Muscle
 Kidney Antibodies  >1032 publications with FRAP and Kidney
 Vascular Antibodies  >930 publications with FRAP and Vascular
 Brain Antibodies  >893 publications with FRAP and Brain
 Liver Antibodies  >692 publications with FRAP and Liver
 Lung Antibodies  >601 publications with FRAP and Lung
 Heart Antibodies  >430 publications with FRAP and Heart
 Bone Antibodies  >321 publications with FRAP and Bone
 Prostate Antibodies  >309 publications with FRAP and Prostate
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