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anti-GAPDH antibody :: Mouse GAPDH Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS462002
Testing Data
Unit / Price
0.1 mg  /  $285 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GAPDH, Monoclonal Antibody

★Popular Item★
 Also Known As   

Mouse anti GAPDH antibody

 Product Synonym Names    Mouse anti-GAPDH
 Product Gene Name   

anti-GAPDH antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 4; NC_005103.2 (161282215..161286090, complement). Location: 4q42
 3D Structure    ModBase 3D Structure for P04797
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 Clonality    Monoclonal
 Clone Number    6C5
 Host    Mouse
 Species Reactivity    Human, Rat, Mouse, Dog, Fish
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 Specificity    This antibody recognizes Glyceraldehyde-3-PDH monomer (36 kDa) and the dimer form. It reacts with human, rabbit, rat, mouse, dog, cat, fish and pig. It does not react with GAPDH from bovine, goat and yeast.
 Purity/Purification    The Mouse IgG1 is purified by Epitope Affinity Purification
 Form/Format    This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
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 Antigen Preparation    A purified rabbit muscle GAPDH (whole molecule).
 Positive Control    Rabbit Muscle
 Cellular Location    Cytoplasmic
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 Preparation and Storage    The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
 Other Notes    Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-GAPDH antibody   

ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

 Application Notes for anti-GAPDH antibody    Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
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 Testing Data of anti-GAPDH antibody    anti-GAPDH antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
 NCBI GI #    122065190
 NCBI GeneID    24383
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 UniProt Primary Accession #    P04797 [Other Products]
 UniProt Secondary Accession #    P09328; Q5M916; Q9QWU4 [Other Products]
 UniProt Related Accession #    P04797; P97617; Q0QEU1 [Other Products]
 Molecular Weight    36 kDa (monomer)
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 NCBI Official Full Name    Glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Synonym Full Names    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Symbol    Gapdh [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    glyceraldehyde-3-phosphate dehydrogenase; BARS-38; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate
 UniProt Protein Name    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Synonym Protein Names   
38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH
 UniProt Gene Name    Gapdh [Similar Products]
 UniProt Synonym Gene Names    Gapd [Similar Products]
 UniProt Entry Name    G3P_RAT
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 NCBI Summary for GAPDH    catalyzes the interconversion of D-glyceraldehyde 3-phosphate, phosphate, and NAD(+) to 3-phospho-D-glyceroyl phosphate and NADH; may contribute to apoptosis [RGD]
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 UniProt Comments for GAPDH    Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Ref.3 Ref.13 Ref.15

Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure: Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct

By similarity. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Ref.13 Ref.14 Ref.15

Subcellular location: Cytoplasm › cytosol. Nucleus. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Ref.13 Ref.14 Ref.15

Tissue specificity: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Ref.9

Post-translational modification: S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus. The effect of S-nitrosylation on enzymatic activity is unclear: according to some authors, it inhibits enzymatic activity and increases endogenous ADP-ribosylation, inhibiting the enzyme in a non-reversible manner (Ref.12). According to others, it does not affect glycolysis (Ref.13). ADP-ribosylation is likely to be a pathophysiological event associated with inhibition of gluconeogenesis.ISGylated

Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
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 Research Articles on GAPDH    1. The glyceraldehyde 3-phosphate dehydrogenase appears to have peroxidase-like properties, but its inhibition failed to impact on downstream oxidant signaling involving secondary protein sulfenation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GAPDH antibodyPathways associated with anti-GAPDH antibody
 Reference Product  PubMed Publications
 Hpcal1 antibody  >1 publications with GAPDH and Hpcal1
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
Diseases associated with anti-GAPDH antibodyOrgans/Tissues associated with anti-GAPDH antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >508 publications with GAPDH and Neoplasms
 Cardiovascular Diseases Antibodies  >191 publications with GAPDH and Cardiovascular Diseases
 Necrosis Antibodies  >121 publications with GAPDH and Necrosis
 Inflammation Antibodies  >104 publications with GAPDH and Inflammation
 Adenocarcinoma Antibodies  >93 publications with GAPDH and Adenocarcinoma
 Leukemia Antibodies  >89 publications with GAPDH and Leukemia
 Neurodegenerative Diseases Antibodies  >88 publications with GAPDH and Neurodegenerative Diseases
 Liver Diseases Antibodies  >86 publications with GAPDH and Liver Diseases
 Kidney Diseases Antibodies  >75 publications with GAPDH and Kidney Diseases
 Lung Diseases Antibodies  >70 publications with GAPDH and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >405 publications with GAPDH and Muscle
 Brain Antibodies  >343 publications with GAPDH and Brain
 Liver Antibodies  >273 publications with GAPDH and Liver
 Heart Antibodies  >211 publications with GAPDH and Heart
 Kidney Antibodies  >185 publications with GAPDH and Kidney
 Lung Antibodies  >156 publications with GAPDH and Lung
 Embryonic Tissue Antibodies  >90 publications with GAPDH and Embryonic Tissue
 Eye Antibodies  >71 publications with GAPDH and Eye
 Connective Tissue Antibodies  >52 publications with GAPDH and Connective Tissue
 Colon Antibodies  >50 publications with GAPDH and Colon
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