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anti-GAPDH antibody :: Mouse GAPDH Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9400221
Western Blot (WB)
Unit / Price
0.05 mL  /  $200 +1 FREE 8GB USB
0.1 mL  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GAPDH, Monoclonal Antibody

 Also Known As   

GAPDH Monoclonal Antibody

 Product Synonym Names    GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
 Product Gene Name   

anti-GAPDH antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    138400
 3D Structure    ModBase 3D Structure for P04406
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 Clonality    Monoclonal
 Host    Mouse
 Species Reactivity    Human, Rat, Mouse, Monkey, Dog, Caenorhabditis Elegans, Hamster, Rabbit, Pig, Sheep
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 Specificity    The antibody detects endogenous GAPDH protein.
 Purity/Purification    The antibody was affinity-purified from antiserum by affinity-chromatography
 Form/Format    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
 Concentration    1 mg/ml (lot specific)
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 Target Name    GAPDH
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 Preparation and Storage    Store at -20 degree C/1 year
 Other Notes    Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-GAPDH antibody    Total protein Ab
 Applications Tested/Suitable for anti-GAPDH antibody   

Western Blot (WB)

 Application Notes for anti-GAPDH antibody    Western blotting: 1:2000-5000
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 Western Blot (WB) of anti-GAPDH antibody    Western blot analysis of Hela, diluted at 1:3000.
anti-GAPDH antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
 NCBI GI #    378404908
 NCBI GeneID    2597
 NCBI Accession #    NP_001243728.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001256799.2 [Other Products]
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 UniProt Primary Accession #    P04406 [Other Products]
 UniProt Secondary Accession #    P00354; Q53X65; E7EUT4 [Other Products]
 UniProt Related Accession #    P04406 [Other Products]
 Molecular Weight    37kd
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 NCBI Official Full Name    glyceraldehyde-3-phosphate dehydrogenase isoform 2
 NCBI Official Synonym Full Names    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Symbol    GAPDH [Similar Products]
 NCBI Official Synonym Symbols   
G3PD; GAPD; HEL-S-162eP
[Similar Products]
 NCBI Protein Information    glyceraldehyde-3-phosphate dehydrogenase
 UniProt Protein Name    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Synonym Protein Names   
Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
 UniProt Gene Name    GAPDH [Similar Products]
 UniProt Synonym Gene Names    GAPD; GAPDH [Similar Products]
 UniProt Entry Name    G3P_HUMAN
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 NCBI Summary for GAPDH    This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
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 UniProt Comments for GAPDH    GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; EC 1.2.1.12

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: microtubule cytoskeleton; nuclear membrane; intracellular membrane-bound organelle; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; lipid particle; nucleus; ribonucleoprotein complex; cytosol; vesicle

Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding

Biological Process: neuron apoptosis; glycolysis; protein stabilization; negative regulation of translation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; microtubule cytoskeleton organization and biogenesis; gluconeogenesis
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 Research Articles on GAPDH    1. The N terminus of nuclear GAPDH binds with PARP-1, and this complex promotes PARP-1 overactivation both in vitro and in vivo.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GAPDH antibodyPathways associated with anti-GAPDH antibody
 Reference Product  PubMed Publications
 RPL13A antibody  >61 publications with GAPDH and RPL13A
 UBC antibody  >60 publications with GAPDH and UBC
 PGK1 antibody  >51 publications with GAPDH and PGK1
 ENO1 antibody  >12 publications with GAPDH and ENO1
 TP53 antibody  >9 publications with GAPDH and TP53
 ALDOA antibody  >9 publications with GAPDH and ALDOA
 HTT antibody  >7 publications with GAPDH and HTT
 BPGM antibody  >1 publications with GAPDH and BPGM
 PGK2 antibody  >1 publications with GAPDH and PGK2
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Diseases associated with anti-GAPDH antibodyOrgans/Tissues associated with anti-GAPDH antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >285 publications with GAPDH and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >257 publications with GAPDH and Cardiovascular Diseases
 Necrosis Antibodies  >215 publications with GAPDH and Necrosis
 Brain Diseases Antibodies  >212 publications with GAPDH and Brain Diseases
 Disease Models, Animal Antibodies  >207 publications with GAPDH and Disease Models, Animal
 Inflammation Antibodies  >193 publications with GAPDH and Inflammation
 Heart Diseases Antibodies  >149 publications with GAPDH and Heart Diseases
 Liver Diseases Antibodies  >143 publications with GAPDH and Liver Diseases
 Kidney Diseases Antibodies  >96 publications with GAPDH and Kidney Diseases
 Liver Neoplasms Antibodies  >79 publications with GAPDH and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >812 publications with GAPDH and Blood
 Muscle Antibodies  >546 publications with GAPDH and Muscle
 Brain Antibodies  >494 publications with GAPDH and Brain
 Liver Antibodies  >408 publications with GAPDH and Liver
 Heart Antibodies  >297 publications with GAPDH and Heart
 Vascular Antibodies  >288 publications with GAPDH and Vascular
 Kidney Antibodies  >257 publications with GAPDH and Kidney
 Lung Antibodies  >231 publications with GAPDH and Lung
 Bone Antibodies  >192 publications with GAPDH and Bone
 Nerve Antibodies  >144 publications with GAPDH and Nerve
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