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anti-GPI antibody :: Mouse anti-Human GPI Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS249250
Unit / Price
0.05 mL  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GPI, Monoclonal Antibody

★Popular Item★
 Also Known As   

Anti-GPI Antibody (clone 4B11) IHC-plus

 Product Synonym Names    Mouse Monoclonal [clone 4B11] (IgG1) to Human GPI; Human GPI; AMF; Autocrine motility factor; Glucose phosphate isomerase; Hexose monophosphate isomerase; Hexosephosphate isomerase; Neuroleukin; Oxoisomerase; Phosphoglucose isomerase; Phosphohexomutase; Phosphohexose isomerase; SA36; PGI; SA-36
 Product Gene Name   

anti-GPI antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    172400
 3D Structure    ModBase 3D Structure for P06744
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    4B11
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Human GPI
 Purity/Purification    Protein A/G purified
 Form/Format    PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
 Concentration    0.5-1.0 mg/mL (lot specific)
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 Immunogen    GPI antibody was raised against full length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell.
 Immunogen Description    Full length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell.
 Immunogen Type    Recombinant protein
 Target Species    Human
 Disclaimer    Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
 Recommended Immunohistochemistry Protocol    The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
 Note    During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
 Warning    This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
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 Preparation and Storage    Store at -20C. Minimize freezing and thawing.
 Other Notes    Small volumes of anti-GPI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GPI antibody

   GPI Antibody, AMF Antibody, Autocrine motility factor Antibody, Glucose phosphate isomerase Antibody, Hexose monophosphate isomerase Antibody, Hexosephosphate isomerase Antibody, Neuroleukin Antibody, Oxoisomerase Antibody, Phosphoglucose isomerase Antibody, Phosphohexomutase Antibody, Phosphohexose isomerase Antibody, SA36 Antibody, PGI Antibody, SA-36 Antibody, Glucose-6-phosphate isomerase Antibody, GNPI Antibody, PHI Antibody, Phosphosaccharomutase Antibody, Sperm antigen 36 Antibody, Sperm antigen-36 Antibody Description: GPI is a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate.
 Applications Tested/Suitable for anti-GPI antibody   

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)

 Application Notes for anti-GPI antibody    IF (1:100)
IHC-P (10 ug/ml)
WB (1:2000)

Optimal dilution to be determined by the researcher.
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 Immunohistochemistry (IHC) of anti-GPI antibody    Anti-GPI antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-GPI antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-GPI antibody    Anti-GPI mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPI.
anti-GPI antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-GPI antibody    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPI.
anti-GPI antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for GPI. It may not necessarily be applicable to this product.
 NCBI GI #    18201905
 NCBI GeneID    2821
 NCBI Accession #    NP_000166.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000175.3 [Other Products]
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 UniProt Primary Accession #    P06744 [Other Products]
 UniProt Secondary Accession #    Q9BRD3; Q9BSK5; Q9UHE6; B4DG39 [Other Products]
 UniProt Related Accession #    P06744 [Other Products]
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 NCBI Official Full Name    glucose-6-phosphate isomerase isoform 2
 NCBI Official Synonym Full Names    glucose-6-phosphate isomerase
 NCBI Official Symbol    GPI [Similar Products]
 NCBI Official Synonym Symbols   
AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
[Similar Products]
 NCBI Protein Information    glucose-6-phosphate isomerase
 UniProt Protein Name    Glucose-6-phosphate isomerase
 UniProt Synonym Protein Names   
Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36
 Protein Family    GPI-anchored protein
 UniProt Gene Name    GPI [Similar Products]
 UniProt Synonym Gene Names    GPI; AMF; NLK; PGI; PHI; SA-36 [Similar Products]
 UniProt Entry Name    G6PI_HUMAN
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 NCBI Summary for GPI    This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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 UniProt Comments for GPI    G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Protein type: Apoptosis; EC 5.3.1.9; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Cytokine; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; cytosol; extracellular space; membrane; neuron projection; nucleoplasm; plasma membrane

Molecular Function: cytokine activity; glucose-6-phosphate isomerase activity; growth factor activity; intramolecular transferase activity; monosaccharide binding; ubiquitin protein ligase binding

Biological Process: aldehyde catabolic process; angiogenesis; carbohydrate metabolic process; gene expression; gluconeogenesis; glucose 6-phosphate metabolic process; glucose metabolic process; hemostasis; humoral immune response; learning and/or memory; methylglyoxal biosynthetic process; negative regulation of caspase activity; negative regulation of neuron apoptosis; transcription initiation from RNA polymerase II promoter

Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency
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 Research Articles on GPI    1. High GPI expression is associated with metastatic phenotype of breast cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-GPI antibodyDiseases associated with anti-GPI antibody
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1628 publications with GPI and Nervous System Diseases
 Anemia Antibodies  >548 publications with GPI and Anemia
 Death Antibodies  >534 publications with GPI and Death
 Inflammation Antibodies  >533 publications with GPI and Inflammation
 Disease Models, Animal Antibodies  >502 publications with GPI and Disease Models, Animal
 Necrosis Antibodies  >471 publications with GPI and Necrosis
 Anemia, Hemolytic Antibodies  >462 publications with GPI and Anemia, Hemolytic
 Ovarian Neoplasms Antibodies  >346 publications with GPI and Ovarian Neoplasms
 Neoplasms, Experimental Antibodies  >269 publications with GPI and Neoplasms, Experimental
 Heart Diseases Antibodies  >267 publications with GPI and Heart Diseases
Organs/Tissues associated with anti-GPI antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4042 publications with GPI and Blood
 Brain Antibodies  >2104 publications with GPI and Brain
 Nerve Antibodies  >703 publications with GPI and Nerve
 Bone Antibodies  >650 publications with GPI and Bone
 Muscle Antibodies  >649 publications with GPI and Muscle
 Vascular Antibodies  >546 publications with GPI and Vascular
 Lung Antibodies  >533 publications with GPI and Lung
 Kidney Antibodies  >519 publications with GPI and Kidney
 Liver Antibodies  >501 publications with GPI and Liver
 Ganglia Antibodies  >473 publications with GPI and Ganglia
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