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anti-GZMB antibody :: Mouse GRANZYME B Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS212507
Testing Data
Unit / Price
0.1 mg  /  $455 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GRANZYME B (GZMB), Monoclonal Antibody

 Also Known As   

MOUSE ANTI GRANZYME B

 Product Gene Name   

anti-GZMB antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    123910
 3D Structure    ModBase 3D Structure for P10144
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    GB11
 Host    Mouse
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 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium azide
Preparation
 Buffer Solution    Tris buffered saline
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-GZMB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GZMB antibody

   Mouse anti Granzyme B antibody, clone GB11 recognizes the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death. Mouse anti Granzyme B antibody, clone GB11 is suitable for the detection of Granzyme B expressing cells by flow cytometry. In normal peripheral blood approximately 20% of CD8+ve T cells have been found to express Granzyme B.
 Applications Tested/Suitable for anti-GZMB antibody   

Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP)

 Application Notes for anti-GZMB antibody    ELISA: This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 as detection reagent.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
ELISA: Minimum Dilution: 2ug/ml; Maximum Dilution: 5ug/ml
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
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 Testing Data of anti-GZMB antibody    Sandwich ELISA analysis of human granzyme B using Mouse anti Human granzyme B as a capture reagent and biotinylated Mouse anti Human granzyme B (MBS210398) as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Human plasma samples are diluted i:2 (green) and 1:4 (blue)
anti-GZMB antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.
 NCBI GI #    221625528
 NCBI GeneID    3002
 NCBI Accession #    NP_004122.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004131.4 [Other Products]
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 UniProt Primary Accession #    P10144 [Other Products]
 UniProt Secondary Accession #    Q8N1D2; Q9UCC1 [Other Products]
 UniProt Related Accession #    P10144 [Other Products]
 Molecular Weight    27,716 Da [Similar Products]
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 NCBI Official Full Name    granzyme B
 NCBI Official Synonym Full Names    granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
 NCBI Official Symbol    GZMB [Similar Products]
 NCBI Official Synonym Symbols   
HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
[Similar Products]
 NCBI Protein Information    granzyme B; C11; CTLA-1; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic serine protease B; fragmentin 2; fragmentin-2; human lymphocyte protein
 UniProt Protein Name    Granzyme B
 UniProt Synonym Protein Names   
C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E
 Protein Family    Granzyme
 UniProt Gene Name    GZMB [Similar Products]
 UniProt Synonym Gene Names    CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP [Similar Products]
 UniProt Entry Name    GRAB_HUMAN
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 NCBI Summary for GZMB    Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GZMB    GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.79; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; immunological synapse; nucleus; cytosol

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; immune response; protein processing; proteolysis
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 Research Articles on GZMB    1. Serum concentrations of granzyme B in patients with ovarian cancer were substantially increased in comparison to concentrations in patients with ovarian cystadenomas or ovarian teratomas.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GZMB antibodyPathways associated with anti-GZMB antibody
 Reference Product  PubMed Publications
 PRF1 antibody  >1284 publications with GZMB and PRF1
 CASP3 antibody  >146 publications with GZMB and CASP3
 BID antibody  >62 publications with GZMB and BID
 SERPINB9 antibody  >60 publications with GZMB and SERPINB9
 IL2RA antibody  >51 publications with GZMB and IL2RA
 CASP7 antibody  >20 publications with GZMB and CASP7
 IL2RB antibody  >14 publications with GZMB and IL2RB
 IGF2R antibody  >13 publications with GZMB and IGF2R
 IL2 antibody  >6 publications with GZMB and IL2
 Products by Pathway  Pathway Diagram
 Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies  Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
Diseases associated with anti-GZMB antibodyOrgans/Tissues associated with anti-GZMB antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >318 publications with GZMB and Necrosis
 Inflammation Antibodies  >210 publications with GZMB and Inflammation
 Disease Models, Animal Antibodies  >123 publications with GZMB and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >99 publications with GZMB and Neoplasms, Experimental
 Liver Diseases Antibodies  >80 publications with GZMB and Liver Diseases
 Hepatitis Antibodies  >55 publications with GZMB and Hepatitis
 Brain Diseases Antibodies  >37 publications with GZMB and Brain Diseases
 Breast Neoplasms Antibodies  >35 publications with GZMB and Breast Neoplasms
 Kidney Diseases Antibodies  >31 publications with GZMB and Kidney Diseases
 Atherosclerosis Antibodies  >22 publications with GZMB and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2148 publications with GZMB and Blood
 Lung Antibodies  >163 publications with GZMB and Lung
 Kidney Antibodies  >148 publications with GZMB and Kidney
 Liver Antibodies  >141 publications with GZMB and Liver
 Spleen Antibodies  >139 publications with GZMB and Spleen
 Bone Marrow Antibodies  >96 publications with GZMB and Bone Marrow
 Heart Antibodies  >95 publications with GZMB and Heart
 Intestine Antibodies  >66 publications with GZMB and Intestine
 Pancreas Antibodies  >34 publications with GZMB and Pancreas
 Uterus Antibodies  >21 publications with GZMB and Uterus
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