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anti-GRB2 antibody :: GRB2 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS475234
Western Blot (WB)
Unit / Price
0.1 mL  /  $335 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GRB2, Monoclonal Antibody

★Popular Item★
 Also Known As   

Anti-GRB2 Mouse mAb

 Product Synonym Names    Abundant SRC homology; Adapter protein GRB2; ASH; Ash protein; EGFRBP GRB2; Epidermal growth factor receptor binding protein; Epidermal growth factor receptor binding protein GRB2; GRB 2; GRB2 adapter protein; Grb2; GRB2_HUMAN; Grb3 3; Growth factor receptor bound protein 2; Growth factor receptor bound protein 3; Growth factor receptor-bound protein 2; HT027; MST084; MSTP084; NCKAP2; OTTHUMP00000166096; OTTHUMP00000166097; OTTHUMP00000166098; Protein ASH; SEM5; SH2/SH3 adapter GRB2
 Product Gene Name   

anti-GRB2 antibody

[Similar Products]
 Product Synonym Gene Name    ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    M96995 mRNA
 3D Structure    ModBase 3D Structure for P62993
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    5G3-E7-F11
 Host    Mouse
Source: Human
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Affinity Purified
 Form/Format    Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
 Concentration    4.2mg/mL (lot specific)
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 Immunogen    Recombinant protein of human GRB2.
 Sensitivity    This antibody detects endogenous levels of GRB2 and does not cross-react with related proteins.
 Sequence Similarities    Belongs to the GRB2/sem-5/DRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.
 Subunit Structure    Associates with activated Tyr-phosphorylated EGF receptor/EGFR and PDGF receptors via its SH2 domain. Also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK; probably via the concerted action of both its SH2 and SH3 domains. It also seems to interact with RAS in the signaling pathway leading to DNA synthesis. Binds to and translocates the guanine nucleotide exchange factors SOS. Interacts with phosphorylated TOM1L1 and MET. Interacts with the phosphorylated C-terminus of SH2B2. Interacts with phosphorylated SIT1, LAX1, LAT, LAT2 and LIME1 upon TCR and/or BCR activation. Interacts with NISCH, PTPNS1, REPS2 and the syntrophin SNTA1. Interacts with REPS1 and PIK3C2B via its SH3 domains (By similarity). Interacts with HCV NS5A via its SH3 domains. Interacts with CBL and CBLB. Interacts with AJUBA and CLNK (By similarity). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity). Interacts with SHB, INPP5D/SHIP1, SKAP1 and SKAP2. Forms a complex with MUC1 and SOS1, through interaction of the SH3 domains with SOS1 and the SH2 domain with phosphorylated MUC1. Interacts with PTPN11. Interacts with PRNP and THEMIS (By similarity). Interacts with FLT1 (tyrosine phosphorylated) and KDR. Interacts with PDGFRA (tyrosine phosphorylated); the interaction may be indirect (By similarity). Interacts with RALGPS1 and with HCST. Interacts (via SH3 domain) with HEV ORF3 protein. Interacts with GAPT and PTPRE. Interacts (via SH2 domain) with KIF26A. Interacts (via SH3 2) with GAB2. Interacts with ADAM15. Interacts with THEMIS2. Interacts (via SH2 domain) with AXL and KIT (phosphorylated). Interacts with PTPRJ and BCR. Interacts with FLT4 (tyrosine phosphorylated). Interacts with EPHB1 and SHC1; activates the MAPK/ERK cascade to regulate cell migration. Interacts with PTPN23. Part of a complex including TNK2, GRB2 and one receptor tyrosine kinase (RTK) such as AXL, in which GRB2 promotes RTK recruitment by TNK2. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts with ERBB4. Interacts with NTRK1 (phosphorylated upon ligand-binding). Interacts with PTK2/FAK1 (tyrosine phosphorylated). Interacts with PTK2B/PYK2 (tyrosine phosphorylated). Isoform 1 interacts with SCIMP. Interacts with PLCG1, LAT and THEMIS upon TCR activation in thymocytes (By similarity). Interacts with TESPA1 and DAB2. Interacts (via SH3 domains) with GAREM isoform 1 (via proline-rich domain and tyrosine phosphorylated); the interaction occurs upon EGF stimulation. Interacts with RAB13; may recruit RAB13 to the leading edge of migrating endothelial cells where it can activate RHOA. Interacts with ASAP3 (phosphorylated form). Interacts with CD28.
 Subcellular Location    Nucleus. Cytoplasm. Endosome. Golgi apparatus.
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 Other Notes    Small volumes of anti-GRB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GRB2 antibody

   Entrez Summary: The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
 Product Categories/Family for anti-GRB2 antibody    Signal Transduction; Adapters; Cytoplasmic; Cancer; Signal transduction; G protein signaling; Small G proteins; Ras family.
 Applications Tested/Suitable for anti-GRB2 antibody   

Western Blot (WB)

 Application Notes for anti-GRB2 antibody    WB: 1:1000
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 Western Blot (WB) of anti-GRB2 antibody    Western blot detection of GRB2 in K562, Raji, Jurkat, Ramos, C6, 3T3 and Hela cell lysates using GRB2 mouse mAb (1:1000 diluted).Predicted band size:25KDa.Observed band size:25KDa.
anti-GRB2 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for GRB2. It may not necessarily be applicable to this product.
 NCBI GI #    4504111
 NCBI GeneID    2885
 NCBI Accession #    NP_002077.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002086 [Other Products]
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 UniProt Primary Accession #    P62993 [Other Products]
 UniProt Secondary Accession #    P29354; Q14450; Q63057; Q63059 [Other Products]
 UniProt Related Accession #    P62993 [Other Products]
 Molecular Weight    25
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 NCBI Official Full Name    growth factor receptor-bound protein 2 isoform 1
 NCBI Official Synonym Full Names    growth factor receptor bound protein 2
 NCBI Official Symbol    GRB2 [Similar Products]
 NCBI Official Synonym Symbols   
ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
[Similar Products]
 NCBI Protein Information    growth factor receptor-bound protein 2
 UniProt Protein Name    Growth factor receptor-bound protein 2
 UniProt Synonym Protein Names   
Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2
 Protein Family    GRB2-related adapter protein
 UniProt Gene Name    GRB2 [Similar Products]
 UniProt Synonym Gene Names    ASH [Similar Products]
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 NCBI Summary for GRB2    The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GRB2    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
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 Research Articles on GRB2    1. The study presents EPR spectroscopic analysis of the binding of the wild-type and mutant Grb2 SH2 domains to the CSpYVNVQC peptide variant. These data confirm that the binding specificity of SH2 domains, even that of Grb2, results from multiple factors and does not depend merely on the nature of one particular residue.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GRB2 antibodyPathways associated with anti-GRB2 antibody
 Reference Product  PubMed Publications
 SHC1 antibody  >284 publications with GRB2 and SHC1
 EGFR antibody  >229 publications with GRB2 and EGFR
 GAB1 antibody  >181 publications with GRB2 and GAB1
 MAPK1 antibody  >174 publications with GRB2 and MAPK1
 CBL antibody  >165 publications with GRB2 and CBL
 PTPN11 antibody  >162 publications with GRB2 and PTPN11
 BCR antibody  >145 publications with GRB2 and BCR
 IRS1 antibody  >137 publications with GRB2 and IRS1
 CRK antibody  >126 publications with GRB2 and CRK
 SOS1 antibody  >122 publications with GRB2 and SOS1
 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
Diseases associated with anti-GRB2 antibodyOrgans/Tissues associated with anti-GRB2 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >483 publications with GRB2 and Neoplasms
 Carcinoma Antibodies  >116 publications with GRB2 and Carcinoma
 Breast Neoplasms Antibodies  >93 publications with GRB2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >44 publications with GRB2 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >44 publications with GRB2 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >37 publications with GRB2 and Cardiovascular Diseases
 Inflammation Antibodies  >32 publications with GRB2 and Inflammation
 Liver Neoplasms Antibodies  >25 publications with GRB2 and Liver Neoplasms
 Liver Diseases Antibodies  >25 publications with GRB2 and Liver Diseases
 Lung Neoplasms Antibodies  >25 publications with GRB2 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >130 publications with GRB2 and Brain
 Liver Antibodies  >89 publications with GRB2 and Liver
 Embryonic Tissue Antibodies  >36 publications with GRB2 and Embryonic Tissue
 Lymph Node Antibodies  >14 publications with GRB2 and Lymph Node
 Placenta Antibodies  >12 publications with GRB2 and Placenta
 Intestine Antibodies  >9 publications with GRB2 and Intestine
 Mammary Gland Antibodies  >9 publications with GRB2 and Mammary Gland
 Uterus Antibodies  >4 publications with GRB2 and Uterus
 Uncharacterized Tissue Antibodies  >4 publications with GRB2 and Uncharacterized Tissue
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