Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow GRLF1 rightarrow Monoclonal GRLF1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Embryonic protein UVS.2 (xhe) Recombinant Protein
 • GIRK1 Antibody
 • stromal cell derived factor 4 (SDF4) ELISA Kit
 • Lactoferrin (LTF) Antibody
 • transmembrane protein 214 (TMEM214) ELISA Kit
 • STAT2 (Phospho-Tyr690) Antibody
 • CCR5 (Phospho-Ser349) Antibody
 • Preprotein translocase subunit SECY, chloroplastic (SECY) (c... Recombinant Protein
 • Frequency clock protein (FRQ), partial Recombinant Protein
 • Aspartyl-glutamyl-tRNA (Asn-Gln) amidotransferase subunit B Recombinant Protein
 • RABGAP1 Antibody
 • Sudan IV (sudan red 4) Biochemical
 • pH-regulated antigen PRA1 (PRA1) Recombinant Protein
 • Fc Fragment Of IgA Receptor (FcaR) Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-GRLF1 antibody :: Mouse anti-Human GRLF1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS120345
Unit / Price
0.1 mg  /  $345 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GRLF1, Monoclonal Antibody

 Also Known As   

Mouse monoclonal antibody Anti-Human GRLF1

 Product Synonym Names    Homo sapiens glucocorticoid receptor DNA binding factor 1; GRF-1; P190A; P190-A; KIAA1722; MGC10745; p190RhoGAP
 Product Gene Name   

anti-GRLF1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    605277
Section Bar
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    2389D3a
 Host    Mouse
 Species Reactivity    Human
Section Bar
 Concentration    100 ug/ml (1.0 ml) (lot specific)
Section Bar
 Preparation    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
 Sterility    Filtered through a 0.22 um membrane.
Section Bar
 Other Notes    Small volumes of anti-GRLF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for anti-GRLF1 antibody

   The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [NCBI Entrez Gene Summary]
 Applications Tested/Suitable for anti-GRLF1 antibody   

Dot Blot (DB)

Section Bar
 Quality Control of anti-GRLF1 antibody    Western blot analysis of immunized recombinant protein, using anti-GRLF1 monoclonal antibody.
anti-GRLF1 antibody Quality Control image
Section Bar
 Quality Control #2 of anti-GRLF1 antibody    Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-GRLF1 antibody Quality Control #2 image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for GRLF1. It may not necessarily be applicable to this product.
 NCBI GI #    25121937
 NCBI GeneID    2909
 NCBI Accession #    NP_077318.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_024342 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q14452; Q9C0E1; A7E2A4 [Other Products]
 UniProt Related Accession #    Q9NRY4 [Other Products]
 Molecular Weight    170,514 Da
Table BarTOPTable Bar
 NCBI Official Synonym Full Names    Rho GTPase activating protein 35
 NCBI Official Symbol    ARHGAP35 [Similar Products]
 NCBI Official Synonym Symbols   
GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
[Similar Products]
 NCBI Protein Information    rho GTPase-activating protein 35
 UniProt Protein Name    Rho GTPase-activating protein 35
 UniProt Synonym Protein Names   
Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A
 UniProt Gene Name    ARHGAP35 [Similar Products]
 UniProt Entry Name    RHG35_HUMAN
Section Bar
 NCBI Summary for GRLF1    The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for GRLF1    GRF-1: the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described.

Protein type: Transcription factor; GAPs; Motility/polarity/chemotaxis; Tumor suppressor; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleus; cytosol; actin cytoskeleton

Molecular Function: DNA binding; GTP binding; transcription corepressor activity

Biological Process: axon guidance; regulation of cell shape; regulation of small GTPase mediated signal transduction; camera-type eye development; transcription, DNA-dependent; negative regulation of Rho protein signal transduction; small GTPase mediated signal transduction; forebrain development; neural tube closure; negative regulation of vascular permeability; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
Table BarTOPTable Bar
 Research Articles on GRLF1    1. these data demonstrate that a complex of p190RhoGAP-A and anillin modulates RhoA-GTP levels in the cytokinetic furrow to ensure progression of cytokinesis.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-GRLF1 antibodyPathways associated with anti-GRLF1 antibody
 Reference Product  PubMed Publications
 RHOA antibody  >31 publications with GRLF1 and RHOA
 SRC antibody  >17 publications with GRLF1 and SRC
 RAC1 antibody  >6 publications with GRLF1 and RAC1
 VAV2 antibody  >3 publications with GRLF1 and VAV2
 RHOB antibody  >2 publications with GRLF1 and RHOB
 RND1 antibody  >2 publications with GRLF1 and RND1
 TIAM1 antibody  >1 publications with GRLF1 and TIAM1
 FYN antibody  >1 publications with GRLF1 and FYN
 RASA1 antibody  >1 publications with GRLF1 and RASA1
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPHA2 Forward Signaling Pathway antibodies  EPHA2 Forward Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Platelet Activation Pathway antibodies  Platelet Activation Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of RhoA Activity Pathway antibodies  Regulation Of RhoA Activity Pathway Diagram
Diseases associated with anti-GRLF1 antibodyOrgans/Tissues associated with anti-GRLF1 antibody
 Disease Name  Pubmed Publications
 Neoplasms, Experimental Antibodies  >4 publications with GRLF1 and Neoplasms, Experimental
 Lung Injury Antibodies  >3 publications with GRLF1 and Lung Injury
 Liver Diseases Antibodies  >3 publications with GRLF1 and Liver Diseases
 Inflammation Antibodies  >2 publications with GRLF1 and Inflammation
 Metaplasia Antibodies  >1 publications with GRLF1 and Metaplasia
 Cardiovascular Diseases Antibodies  >1 publications with GRLF1 and Cardiovascular Diseases
 Liver Neoplasms, Experimental Antibodies  >1 publications with GRLF1 and Liver Neoplasms, Experimental
 Brain Diseases Antibodies  >1 publications with GRLF1 and Brain Diseases
 Hyperplasia Antibodies  >1 publications with GRLF1 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11 publications with GRLF1 and Blood
 Lung Antibodies  >7 publications with GRLF1 and Lung
 Embryonic Tissue Antibodies  >5 publications with GRLF1 and Embryonic Tissue
 Nerve Antibodies  >5 publications with GRLF1 and Nerve
 Liver Antibodies  >4 publications with GRLF1 and Liver
 Brain Antibodies  >4 publications with GRLF1 and Brain
 Mammary Gland Antibodies  >3 publications with GRLF1 and Mammary Gland
 Eye Antibodies  >2 publications with GRLF1 and Eye
 Muscle Antibodies  >2 publications with GRLF1 and Muscle
 Bone Antibodies  >2 publications with GRLF1 and Bone
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions