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anti-GFAP antibody :: Rabbit Glial Fibrillary Acidic Protein Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS302760
Immunohistochemistry (IHC)
Unit / Price
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 Product Name   

Glial Fibrillary Acidic Protein (GFAP), Monoclonal Antibody

★Popular Item★
 Also Known As   

Rabbit Anti-Human GFAP Monoclonal Antibody (Clone SP78)

 Product Gene Name   

anti-GFAP antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    137780
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    SP78
 Host    Rabbit
 Species Reactivity    Human
Predicted Reactivity: Bovine, Dog, Mouse, Pig, Rabbit, Rat
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 Form/Format    0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
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 Immunogen    Synthetic peptide corresponding to C-terminus of human GFAP protein.
 Control Tissue    Astrocytoma
 Cellular Localization    Cytoplasm
 Entrez Gene Code    2670
 Pathway    Stem Cell
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 Preparation and Storage    Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
 Other Notes    Small volumes of anti-GFAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GFAP antibody

   Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition, many types of brain tumors presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
 Applications Tested/Suitable for anti-GFAP antibody   

Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-GFAP antibody    Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:100
IHC Positive Control: Astrocytoma
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 Immunohistochemistry (IHC) of anti-GFAP antibody    Human astrocytoma stained with anti-GFAP antibody
anti-GFAP antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for GFAP. It may not necessarily be applicable to this product.
 NCBI GI #    196115290
 NCBI GeneID    2670
 NCBI Accession #    NP_001124491.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001131019.2 [Other Products]
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 UniProt Secondary Accession #    Q53H98; Q5D055; Q6ZQS3; Q7Z5J6; Q7Z5J7; Q96KS4; Q96P18; Q9UFD0; B2RD44; D3DX59; E9PAX3 [Other Products]
 UniProt Related Accession #    P14136 [Other Products]
 Molecular Weight    51-52 kDa
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 NCBI Official Full Name    glial fibrillary acidic protein isoform 2
 NCBI Official Synonym Full Names    glial fibrillary acidic protein
 NCBI Official Symbol    GFAP [Similar Products]
 NCBI Protein Information    glial fibrillary acidic protein
 UniProt Protein Name    Glial fibrillary acidic protein
 Protein Family    Glial fibrillary acidic protein
 UniProt Gene Name    GFAP [Similar Products]
 UniProt Synonym Gene Names    GFAP [Similar Products]
 UniProt Entry Name    GFAP_HUMAN
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 NCBI Summary for GFAP    This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
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 UniProt Comments for GFAP    GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: membrane; cytoplasm; intermediate filament; cytosol

Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding

Biological Process: extracellular matrix organization and biogenesis; Bergmann glial cell differentiation; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development

Disease: Alexander Disease
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 Research Articles on GFAP    1. There are significant increases in glial fibrillary acidic protein levels in children undergoing cardiopulmonary bypass for repair of congenital heart disease. The highest values were seen during the re-warming phase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-GFAP antibodyDiseases associated with anti-GFAP antibody
 Products by Pathway  Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Nuclear Signaling By ERBB4 Pathway antibodies  Nuclear Signaling By ERBB4 Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By ERBB4 Pathway antibodies  Signaling By ERBB4 Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4908 publications with GFAP and Nervous System Diseases
 Brain Diseases Antibodies  >3706 publications with GFAP and Brain Diseases
 Disease Models, Animal Antibodies  >1433 publications with GFAP and Disease Models, Animal
 Gliosis Antibodies  >1111 publications with GFAP and Gliosis
 Cardiovascular Diseases Antibodies  >877 publications with GFAP and Cardiovascular Diseases
 Brain Injuries Antibodies  >700 publications with GFAP and Brain Injuries
 Inflammation Antibodies  >640 publications with GFAP and Inflammation
 Necrosis Antibodies  >573 publications with GFAP and Necrosis
 Nerve Degeneration Antibodies  >541 publications with GFAP and Nerve Degeneration
 Epilepsy Antibodies  >365 publications with GFAP and Epilepsy
Organs/Tissues associated with anti-GFAP antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7873 publications with GFAP and Brain
 Nerve Antibodies  >3399 publications with GFAP and Nerve
 Blood Antibodies  >1592 publications with GFAP and Blood
 Spinal Cord Antibodies  >1387 publications with GFAP and Spinal Cord
 Eye Antibodies  >995 publications with GFAP and Eye
 Muscle Antibodies  >516 publications with GFAP and Muscle
 Ganglia Antibodies  >296 publications with GFAP and Ganglia
 Bone Marrow Antibodies  >253 publications with GFAP and Bone Marrow
 Liver Antibodies  >207 publications with GFAP and Liver
 Connective Tissue Antibodies  >187 publications with GFAP and Connective Tissue
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