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anti-Grin2b antibody :: Mouse GluN2B/NR2B Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS800075
Unit / Price
0.1 mg  /  $390 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GluN2B/NR2B (Grin2b), Monoclonal Antibody

★Popular Item★
 Also Known As   

Mouse Anti-Rat GluN2B/NR2B Monoclonal IgG2b

 Product Synonym Names    GluN2B/NR2B Antibody, 559-36; NMDAR2B; hNR3; MGC142180; glutamate receptor subunit epsilon-2; NR3
 Product Gene Name   

anti-Grin2b antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q00960
 Clonality    Monoclonal
 Isotype    IgG2b
 Clone Number    S59-36
 Host    Mouse
 Species Reactivity    Human, Rat, Mouse
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 Specificity    Detects ~166kDa. No cross-reactivity against NR2A
 Form/Format    Protein G Purified
 Concentration    1mg/mL (lot specific)
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 Certificate of Analysis    1ug/mL was sufficient for detection of NR2B in 10ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
 Immunogen    Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B.
 Buffer    PBS pH7.4, 50% glycerol, 0.09% sodium azide
 Cellular Localization    Cell membrane, Cell Junction, synapse, postsynaptic cell membrane
 Research Areas    Neuroscience, Ion Channels, Neurotransmitter Receptors, Glutamate Receptors, NMDA Receptors, Neurodegeneration.
 Conjugates    Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
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 Preparation and Storage    Store at -20C. 1 year+.
Avoid freeze/thaw cycles.
Shipping Temperature: 4C
 Other Notes    Small volumes of anti-Grin2b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Grin2b antibody

   Background Info: Detects ~166kDa. No cross-reactivity against NR2A

Scientific Background: NR2B containing receptors have been implicated in synaptic plasticity, memory formation and pain modulation (1). Studies suggest that the NR2B subunit of glutamate receptors may be potential targets for relieving pain; NR2B may be a probable target for anti-nociceptive drugs, and may also be useful as analgesics (2).
 Product Categories/Family for anti-Grin2b antibody    Ion Channels, Neuroscience
 Applications Tested/Suitable for anti-Grin2b antibody   

WB, IHC, ICC/IF, IP, AM

 Application Notes for anti-Grin2b antibody    WB: (1:1000)
IHC: (1:1000)
ICC/IF (1:1000).

Optimal dilutions for assays should be determined by the user.
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 Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Grin2b antibody    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.
anti-Grin2b antibody Immunocytochemistry/Immunofluorescence (ICC/IF) (ICC) image
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 Western Blot (WB) of anti-Grin2b antibody    Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.
anti-Grin2b antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-Grin2b antibody    Immunohistochemistry analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.
anti-Grin2b antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-Grin2b antibody    Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
anti-Grin2b antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for Grin2b. It may not necessarily be applicable to this product.
 NCBI GI #    6980984
 NCBI GeneID    24410
 NCBI Accession #    NP_036706.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012574.1 [Other Products]
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 UniProt Primary Accession #    Q00960 [Other Products]
 UniProt Secondary Accession #    Q62684 [Other Products]
 UniProt Related Accession #    Q00960 [Other Products]
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 NCBI Official Full Name    glutamate receptor ionotropic, NMDA 2B
 NCBI Official Synonym Full Names    glutamate receptor, ionotropic, N-methyl D-aspartate 2B
 NCBI Official Symbol    Grin2b [Similar Products]
 NCBI Official Synonym Symbols   
GluN2B
[Similar Products]
 NCBI Protein Information    glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
 UniProt Protein Name    Glutamate receptor ionotropic, NMDA 2B
 UniProt Synonym Protein Names   
Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B
 Protein Family    Glutamate receptor ionotropic
 UniProt Gene Name    Grin2b [Similar Products]
 UniProt Synonym Gene Names    GluN2B; NMDAR2B; NR2B [Similar Products]
 UniProt Entry Name    NMDE2_RAT
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 NCBI Summary for Grin2b    subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]
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 UniProt Comments for Grin2b    NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

Protein type: Membrane protein, multi-pass; Channel, calcium; Channel, ligand-gated; Membrane protein, integral

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; postsynaptic density; dendritic spine; terminal button; synapse; cell junction; Z disc; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: neurotransmitter binding; interleukin-1 receptor binding; zinc ion binding; calcium channel activity; beta-catenin binding; drug binding; ionotropic glutamate receptor binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding; cell adhesion molecule binding; cation channel activity; receptor binding; D2 dopamine receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; behavioral fear response; startle response; response to toxin; rhythmic process; positive regulation of glutamate secretion; regulation of synaptic plasticity; response to other organism; receptor clustering; response to organic cyclic substance; positive regulation of synaptic transmission; response to carbohydrate stimulus; synaptic transmission; behavioral response to pain; response to magnesium ion; learning and/or memory; calcium ion transport; response to methylmercury; response to electrical stimulus; associative learning; cation transport; cellular response to magnesium starvation; synaptic transmission, glutamatergic; regulation of action potential; in utero embryonic development; hippocampus development; response to amphetamine; learning; response to cocaine; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; sensory organ development; regulation of postsynaptic membrane potential; response to ethanol; suckling behavior; long-term memory; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; response to cytokine stimulus; regulation of MAPKKK cascade; cerebral cortex development; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; response to amine stimulus
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Product References and Citations for anti-Grin2b antibody

   1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Zhuo M. (2009) Mol Brain. 2(1): 4 4. Gurwitz D, and Weizman A. (2002) Drug Discovery Today, 7(7): 403-406.
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 Research Articles on Grin2b    1. GluN2B receptor subtype decreases in the perirhinal cortex of methamphetamine induced-memory deficit animals.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Grin2b antibodyPathways associated with anti-Grin2b antibody
 Reference Product  PubMed Publications
 Grin2a antibody  >30 publications with Grin2b and Grin2a
 Grin1 antibody  >22 publications with Grin2b and Grin1
 Grik2 antibody  >5 publications with Grin2b and Grik2
 Grin3b antibody  >4 publications with Grin2b and Grin3b
 Grin3a antibody  >3 publications with Grin2b and Grin3a
 Grik1 antibody  >2 publications with Grin2b and Grik1
 Dlg4 antibody  >1 publications with Grin2b and Dlg4
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Diseases associated with anti-Grin2b antibody
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >83 publications with Grin2b and Mental Disorders
 Schizophrenia Antibodies  >40 publications with Grin2b and Schizophrenia
 Nervous System Diseases Antibodies  >33 publications with Grin2b and Nervous System Diseases
 Brain Diseases Antibodies  >20 publications with Grin2b and Brain Diseases
 Anxiety Disorders Antibodies  >11 publications with Grin2b and Anxiety Disorders
 Movement Disorders Antibodies  >11 publications with Grin2b and Movement Disorders
 Child Development Disorders, Pervasive Antibodies  >9 publications with Grin2b and Child Development Disorders, Pervasive
 Cognition Disorders Antibodies  >9 publications with Grin2b and Cognition Disorders
 Epilepsy Antibodies  >9 publications with Grin2b and Epilepsy
 Neurobehavioral Manifestations Antibodies  >8 publications with Grin2b and Neurobehavioral Manifestations
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