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anti-GAPDH antibody :: anti-Human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS311241 anti-GAPDH antibody
Unit / Price
1 mg  /  $565 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Monoclonal Antibody

 Also Known As   

MAb to Glyceraldehyde-3-PDH

 Product Synonym Names    Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
 Product Gene Name   

anti-GAPDH antibody

[Similar Products]
 Matching Pairs    Capture Antibody: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Clone #6F7 (MBS311080)
Detection Antibody: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Clone #B903M (MBS311241)
 Matching Pairs    Capture Antibody: Glyceraldehyde-3-Phosphate Dehydrogenase (multiple species) (GAPDH) Clone #6C5 (MBS311206)
Detection Antibody: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Clone #B903M (MBS311241)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 12; NC_000012.11 (6643657..6647536). Location: 12p13
 OMIM    138400
 3D Structure    ModBase 3D Structure for P04406
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    B903M
 Host    Host: Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/c mice.
Source: Ascites
 Species Reactivity    Human
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 Specificity    Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Cross-reacts with GAPDH from Human, bovine, porcine, goat, rabbit and rat.
 Purity/Purification    >90% pure (SDS-PAGE). Protein A chromatography
 Form/Format    Purified, Liquid
 Concentration    6.7mg/ml (Sigma protein assay kit) (lot specific)
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 Immunogen    Human or rabbit GAPDH
 Affinity Constant    Not determined
 Buffer    PBS, pH 7.4
 Preservative    0.1% Sodium azide
 Important Note    Centrifuge before opening to ensure complete recovery of vial contents.
 Warnings    This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
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 Preparation and Storage    Store at 2 to 8 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-GAPDH antibody    Monoclonal Antibodies to Enzymes
 Applications Tested/Suitable for anti-GAPDH antibody   

EIA/ELISA, Immunocytochemistry, Immunoassay Antibody Pairs, Western Blot

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NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
 NCBI GI #    7669492
 NCBI GeneID    2597
 NCBI Accession #    NP_002037.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002046.3 [Other Products]
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 UniProt Primary Accession #    P04406 [Other Products]
 UniProt Secondary Accession #    P00354; Q53X65 [Other Products]
 UniProt Related Accession #    P04406; Q0QET7; Q16768; Q2TSD0; Q5ZEY3 [Other Products]
 Molecular Weight    36,053 Da [Similar Products]
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 NCBI Official Full Name    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Synonym Full Names    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Symbol    GAPDH [Similar Products]
 NCBI Official Synonym Symbols   
G3PD; GAPD; MGC88685
[Similar Products]
 NCBI Protein Information    glyceraldehyde-3-phosphate dehydrogenase; OTTHUMP00000174430; OTTHUMP00000174431; OTTHUMP00000174432; OTTHUMP00000174434; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase
 UniProt Protein Name    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Synonym Protein Names   
Peptidyl-cysteine S-nitrosylase GAPDH
 Protein Family    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Gene Name    GAPDH [Similar Products]
 UniProt Synonym Gene Names    GAPD [Similar Products]
 UniProt Entry Name    G3P_HUMAN
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 NCBI Summary for GAPDH    The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
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 UniProt Comments for GAPDH    Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC

By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Ref.6 Ref.23

Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Ref.6

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation

By similarity. Interacts with EIF1AD, USP25, PRKCI and WARS. Ref.23 Ref.25 Ref.28 Ref.36 Ref.39 Ref.40

Subcellular location: Cytoplasm › cytosol. Nucleus

By similarity. Cytoplasm › perinuclear region. Membrane. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal

By similarity. Postnuclear and Perinuclear regions. Ref.24

Post-translational modification: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus

By similarity.ISGylated

Probable. Ref.29

Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
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 Research Articles on GAPDH    1. In MCF-7 cells, the activation of HIF1 on the novel hypoxia response element contributes to the breast cancer-specific hypoxic induction of GAPDH gene expression and absolutely depends on the presence of Sp1 on the GC-box.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GAPDH antibodyPathways associated with anti-GAPDH antibody
 Reference Product  PubMed Publications
 ACTB antibody  >128 publications with GAPDH and ACTB
 PGK1 antibody  >28 publications with GAPDH and PGK1
 SIAH1 antibody  >11 publications with GAPDH and SIAH1
 APP antibody  >9 publications with GAPDH and APP
 EGFR antibody  >8 publications with GAPDH and EGFR
 HTT antibody  >6 publications with GAPDH and HTT
 SLC2A1 antibody  >5 publications with GAPDH and SLC2A1
 SLC2A4 antibody  >3 publications with GAPDH and SLC2A4
 S100A6 antibody  >2 publications with GAPDH and S100A6
 PRDX1 antibody  >1 publications with GAPDH and PRDX1
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
Diseases associated with anti-GAPDH antibodyOrgans/Tissues associated with anti-GAPDH antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >508 publications with GAPDH and Neoplasms
 Cardiovascular Diseases Antibodies  >191 publications with GAPDH and Cardiovascular Diseases
 Necrosis Antibodies  >121 publications with GAPDH and Necrosis
 Inflammation Antibodies  >104 publications with GAPDH and Inflammation
 Adenocarcinoma Antibodies  >93 publications with GAPDH and Adenocarcinoma
 Leukemia Antibodies  >89 publications with GAPDH and Leukemia
 Neurodegenerative Diseases Antibodies  >89 publications with GAPDH and Neurodegenerative Diseases
 Liver Diseases Antibodies  >86 publications with GAPDH and Liver Diseases
 Kidney Diseases Antibodies  >75 publications with GAPDH and Kidney Diseases
 Lung Diseases Antibodies  >70 publications with GAPDH and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >586 publications with GAPDH and Blood
 Muscle Antibodies  >405 publications with GAPDH and Muscle
 Brain Antibodies  >343 publications with GAPDH and Brain
 Liver Antibodies  >273 publications with GAPDH and Liver
 Heart Antibodies  >211 publications with GAPDH and Heart
 Vascular Antibodies  >203 publications with GAPDH and Vascular
 Kidney Antibodies  >185 publications with GAPDH and Kidney
 Lung Antibodies  >156 publications with GAPDH and Lung
 Bone Antibodies  >129 publications with GAPDH and Bone
 Nerve Antibodies  >99 publications with GAPDH and Nerve
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