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anti-GAPDH antibody :: Mouse Glyceraldehyde-3-Phosphate Dehydrogenase Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS140362 anti-GAPDH antibody
Unit / Price
0.005 mg  /  $140 +1 FREE 8GB USB
0.02 mg  /  $205 +1 FREE 8GB USB
0.1 mg  /  $505 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Monoclonal Antibody

 Also Known As   

Mouse Anti Human Glyceraldehyde-3-Phosphate Dehydrogenase

 Product Synonym Names    GAPDH Antibody; Glyceraldehyde-3-Phosphate Dehydrogenase, Mouse Anti Human; G3PD; GAPD; MGC88685; GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase
 Product Gene Name   

anti-GAPDH antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG2b heavy chain and kappa light chain
 Clone Number    PAT8G4AT
 Host    Mouse
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 Purity/Purification    GAPDH antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
 Form/Format    1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sterile Filtered clear solution.
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 Immunogen    Anti-human GAPDH mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GAPDH amino acids 1-335 purified from E Coli.
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 Preparation and Storage    For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
 Other Notes    Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GAPDH antibody

   Introduction: GAPDH is a catalytic enzyme normally known to play a role in glycolysis. GAPDH exists as a tetramer composed of 36-kDa subunits and has a range of intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, GAPDH has function in intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. GAPDH catalyzes a vital energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
 Product Categories/Family for anti-GAPDH antibody    MONOCLONAL ANTIBODIES, ANTI-HUMAN ENZYMES; Monoclonal Antibodies; Anti Human Enzyme
 Applications Tested/Suitable for anti-GAPDH antibody   

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

 Application Notes for anti-GAPDH antibody    GAPDH antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 10,000. Recommended starting dilution is 1:1,000.
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NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
 NCBI GI #    8393418
 NCBI GeneID    24383
 NCBI Accession #    NP_058704.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_017008.4 [Other Products]
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 UniProt Secondary Accession #    P09328; Q5M916; Q9QWU4 [Other Products]
 UniProt Related Accession #    P04797 [Other Products]
 Molecular Weight    35,828 Da
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 NCBI Official Full Name    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Synonym Full Names    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Symbol    Gapdh [Similar Products]
 NCBI Official Synonym Symbols   
Gapd; BARS-38
[Similar Products]
 NCBI Protein Information    glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; peptidyl-cysteine S-nitrosylase GAPDH
 UniProt Protein Name    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Synonym Protein Names   
38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
 Protein Family    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Gene Name    Gapdh [Similar Products]
 UniProt Synonym Gene Names    Gapd; GAPDH [Similar Products]
 UniProt Entry Name    G3P_RAT
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 NCBI Summary for GAPDH    This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]
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 UniProt Comments for GAPDH    GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma

Protein type: EC 1.2.1.12; Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Cellular Component: microtubule cytoskeleton; nuclear membrane; membrane; mitochondrion; intracellular membrane-bound organelle; cytoplasm; plasma membrane; lipid particle; cytosol; nucleus; ribonucleoprotein complex; vesicle

Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding

Biological Process: regulation of translation; neuron apoptosis; glycolysis; protein stabilization; apoptosis; multicellular organismal development; negative regulation of translation; carbohydrate metabolic process; microtubule cytoskeleton organization and biogenesis; gluconeogenesis
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 Research Articles on GAPDH    1. The nuclear GAPDH forms a protein complex with p53 and enhances p53 expression and phosphorylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GAPDH antibodyPathways associated with anti-GAPDH antibody
 Reference Product  PubMed Publications
 Actb antibody  >243 publications with GAPDH and Actb
 Pgk1 antibody  >48 publications with GAPDH and Pgk1
 Egfr antibody  >16 publications with GAPDH and Egfr
 Tpi1 antibody  >7 publications with GAPDH and Tpi1
 Akt1 antibody  >3 publications with GAPDH and Akt1
 Il2 antibody  >2 publications with GAPDH and Il2
 Fn1 antibody  >1 publications with GAPDH and Fn1
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
Diseases associated with anti-GAPDH antibodyOrgans/Tissues associated with anti-GAPDH antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >276 publications with GAPDH and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >250 publications with GAPDH and Cardiovascular Diseases
 Necrosis Antibodies  >207 publications with GAPDH and Necrosis
 Brain Diseases Antibodies  >204 publications with GAPDH and Brain Diseases
 Disease Models, Animal Antibodies  >199 publications with GAPDH and Disease Models, Animal
 Inflammation Antibodies  >187 publications with GAPDH and Inflammation
 Heart Diseases Antibodies  >147 publications with GAPDH and Heart Diseases
 Liver Diseases Antibodies  >137 publications with GAPDH and Liver Diseases
 Kidney Diseases Antibodies  >92 publications with GAPDH and Kidney Diseases
 Liver Neoplasms Antibodies  >78 publications with GAPDH and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >527 publications with GAPDH and Muscle
 Brain Antibodies  >480 publications with GAPDH and Brain
 Liver Antibodies  >394 publications with GAPDH and Liver
 Heart Antibodies  >289 publications with GAPDH and Heart
 Kidney Antibodies  >250 publications with GAPDH and Kidney
 Artery Antibodies  >123 publications with GAPDH and Artery
 Embryonic Tissue Antibodies  >121 publications with GAPDH and Embryonic Tissue
 Eye Antibodies  >90 publications with GAPDH and Eye
 Connective Tissue Antibodies  >87 publications with GAPDH and Connective Tissue
 Colon Antibodies  >71 publications with GAPDH and Colon
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