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anti-GreB antibody :: Mouse anti-E Coli GreB Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS680010 anti-GreB antibody
Unit / Price
0.1 mg  /  $380 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GreB, Monoclonal Antibody

 Also Known As   

mAb to GreB

 Product Synonym Names    E. coli Gre B Monoclonal Antibody; Transcription elongation factor greB; Transcript cleavage factor greB
 Product Gene Name   

anti-GreB antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    NC_000913.2 (3534834..3535310)
 3D Structure    ModBase 3D Structure for P30128
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    1C11H9
 Host    Mouse
 Species Reactivity    E Coli
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 Specificity    GreB (E. coli GreB)
 Form/Format    Affinity Purified
 Concentration    1 mg/ml in PBS pH 7.2 (lot specific)
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 Immunogen    Recombinant Protein
 Reconstitution    Add 100ul of ddH2O to the tube containing antibody and mix to dissolve
 Product Note    *Cross reactivity and Applications data is per published literature and/or unpublished data.
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 Preparation and Storage    Store at -20 degree C lyophilized, 4 degree C Reconstituted or sub-aliquot and -20 degree C.
 Other Notes    Small volumes of anti-GreB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-GreB antibody

   mAb to Gre B. Gre B plays a role in E. coli Transcription
Gre B is a 18.5 kDa E. coli transcription factor
18.5 kDa E. coli transcription factor

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as greA or greB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length.
 Applications Tested/Suitable for anti-GreB antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-GreB antibody    ELISA (EIA): Positive
Western Blot: Yes - dilute at 1:1000
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NCBI/Uniprot data below describe general gene information for GreB. It may not necessarily be applicable to this product.
 NCBI GI #    90111587
 NCBI GeneID    2847706
 NCBI Accession #    YP_026216 [Other Products]
 NCBI GenBank Nucleotide #    NC_000913.2 [Other Products]
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 UniProt Primary Accession #    P30128 [Other Products]
 UniProt Secondary Accession #    P78114; Q2M771 [Other Products]
 Molecular Weight    18,527 Da
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 NCBI Official Full Name    transcript cleavage factor
 NCBI Official Symbol    greB [Similar Products]
 NCBI Official Synonym Symbols   
ECK3393; JW3369
[Similar Products]
 NCBI Protein Information    transcript cleavage factor
 UniProt Protein Name    Transcription elongation factor GreB
 UniProt Synonym Protein Names   
Transcript cleavage factor GreB
 Protein Family    GREB1-like protein
 UniProt Gene Name    greB [Similar Products]
 UniProt Entry Name    GREB_ECOLI
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 NCBI Summary for GreB    GreB stimulates the mRNA cleavage activity of RNA polymerase , which acts to release a polymerase complex that has stalled . [More information is available at EcoCyc: EG11578].
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 UniProt Comments for GreB    Function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length. Ref.1

Sequence similarities: Belongs to the GreA/GreB family. GreB subfamily.

Sequence caution: The sequence AAA58203.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
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 Research Articles on GreB    1. Results show that although DksA and GreB compete for binding to free RNA polymerase, they do not interfere with each other's activities during elongation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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