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anti-ERBB2 antibody :: Mouse anti-Human, Rat. HER-2 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS438027 anti-ERBB2 antibody
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HER-2 (ERBB2), Monoclonal Antibody

★Popular Item★
 Also Known As   

HER-2, Mouse Monoclonal Antibody [clone HRB2/258]

 Product Synonym Names    p185; CD340; Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog
 Product Gene Name   

anti-ERBB2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    613659
 3D Structure    ModBase 3D Structure for P04626
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 Clonality    Monoclonal
 Isotype    IgG2a, kappa
 Clone Number    HRB2/258
 Host    Mouse
 Species Reactivity    Human, Rat.
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 Specificity    Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. C-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
 Form/Format    Supplied as 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
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 Localization    Cell surface
 Positive Control    SKBR-3 Cells or Breast Cancers.
 Human Chromosomal Location    17q12
 Immunogen    Recombinant extracellular domain of human HER-2 protein
 Quantity and Buffer    0.5 ml at 200ug/ml with BSA and azide
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 Preparation and Storage    Store at 2 to 8 degree C. Antibody is stable for 24 months.
 Other Notes    Small volumes of anti-ERBB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-ERBB2 antibody   

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)

 Application Notes for anti-ERBB2 antibody    ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Western Blotting (0.5-1ug/ml); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.
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NCBI/Uniprot data below describe general gene information for ERBB2. It may not necessarily be applicable to this product.
 NCBI GI #    54792098
 NCBI GeneID    2064
 NCBI Accession #    NP_001005862.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001005862.2 [Other Products]
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 UniProt Primary Accession #    P04626 [Other Products]
 UniProt Secondary Accession #    Q14256; Q6LDV1; Q9UMK4; B2RZG3; B4DHN3; X5D2V5 [Other Products]
 UniProt Related Accession #    P04626 [Other Products]
 Molecular Weight    185kDa
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 NCBI Official Full Name    receptor tyrosine-protein kinase erbB-2 isoform b
 NCBI Official Synonym Full Names    v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
 NCBI Official Symbol    ERBB2 [Similar Products]
 NCBI Official Synonym Symbols   
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
[Similar Products]
 NCBI Protein Information    receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
 UniProt Protein Name    Receptor tyrosine-protein kinase erbB-2
 UniProt Synonym Protein Names   
Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen: CD340
 Protein Family    Receptor tyrosine-protein kinase
 UniProt Gene Name    ERBB2 [Similar Products]
 UniProt Synonym Gene Names    HER2; MLN19; NEU; NGL; MLN 19 [Similar Products]
 UniProt Entry Name    ERBB2_HUMAN
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 NCBI Summary for ERBB2    This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ERBB2    HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: Membrane protein, integral; Oncoprotein; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; EGFR family

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: basolateral plasma membrane; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; cytoplasmic vesicle; nucleus; receptor complex

Molecular Function: protein C-terminus binding; protein dimerization activity; identical protein binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; protein binding; transmembrane receptor activity; ErbB-3 class receptor binding; protein heterodimerization activity; growth factor binding; protein-tyrosine kinase activity; ATP binding

Biological Process: myelination; axon guidance; phosphoinositide 3-kinase cascade; peptidyl-tyrosine phosphorylation; positive regulation of cell adhesion; wound healing; positive regulation of translation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; heart development; motor axon guidance; signal transduction; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; positive regulation of transcription from RNA polymerase I promoter; positive regulation of MAP kinase activity; cell surface receptor linked signal transduction; oligodendrocyte differentiation; neuromuscular junction development; epidermal growth factor receptor signaling pathway; negative regulation of immature T cell proliferation in the thymus; regulation of microtubule-based process; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; positive regulation of cell growth; regulation of angiogenesis; peripheral nervous system development; cell proliferation; innate immune response; positive regulation of transcription from RNA polymerase III promoter; positive regulation of protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of epithelial cell proliferation

Disease: Gastric Cancer; Lung Cancer; Glioma Susceptibility 1
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Product References and Citations for anti-ERBB2 antibody

   Utrilla JC, et al. Histopathology. 34: 60-65 (1999).
2. Wright C, et al. British Journal of Cancer. 65: 118-121 (1992).
3. Corbett IP, et al. Journal of Pathology. 161: 15-25 (1990).
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 Research Articles on ERBB2    1. HER2 was positive in nearly 52 % of the primary tumors, and these expressed HER2 in corresponding lymph node and liver metastases in 93 and 100 % of the cases, respectively
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ERBB2 antibodyPathways associated with anti-ERBB2 antibody
 Reference Product  PubMed Publications
 EGFR antibody  >2877 publications with ERBB2 and EGFR
 ERBB3 antibody  >796 publications with ERBB2 and ERBB3
 SRC antibody  >389 publications with ERBB2 and SRC
 GRB2 antibody  >109 publications with ERBB2 and GRB2
 GRB7 antibody  >81 publications with ERBB2 and GRB7
 ERBB2IP antibody  >43 publications with ERBB2 and ERBB2IP
 SHC1 antibody  >34 publications with ERBB2 and SHC1
 UBC antibody  >3 publications with ERBB2 and UBC
 HSP90AA1 antibody  >1 publications with ERBB2 and HSP90AA1
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 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
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 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
Diseases associated with anti-ERBB2 antibodyOrgans/Tissues associated with anti-ERBB2 antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >12145 publications with ERBB2 and Breast Neoplasms
 Skin Diseases Antibodies  >11293 publications with ERBB2 and Skin Diseases
 Carcinoma Antibodies  >6595 publications with ERBB2 and Carcinoma
 Adenocarcinoma Antibodies  >3464 publications with ERBB2 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >2855 publications with ERBB2 and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >1676 publications with ERBB2 and Neoplasms, Experimental
 Disease Progression Antibodies  >1593 publications with ERBB2 and Disease Progression
 Lymphatic Metastasis Antibodies  >1310 publications with ERBB2 and Lymphatic Metastasis
 Gastrointestinal Neoplasms Antibodies  >1272 publications with ERBB2 and Gastrointestinal Neoplasms
 Lung Neoplasms Antibodies  >1170 publications with ERBB2 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2157 publications with ERBB2 and Blood
 Lymph Node Antibodies  >1897 publications with ERBB2 and Lymph Node
 Lung Antibodies  >1379 publications with ERBB2 and Lung
 Stomach Antibodies  >750 publications with ERBB2 and Stomach
 Brain Antibodies  >646 publications with ERBB2 and Brain
 Liver Antibodies  >571 publications with ERBB2 and Liver
 Prostate Antibodies  >501 publications with ERBB2 and Prostate
 Bone Antibodies  >467 publications with ERBB2 and Bone
 Mammary Gland Antibodies  >448 publications with ERBB2 and Mammary Gland
 Heart Antibodies  >422 publications with ERBB2 and Heart
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