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anti-HK2 antibody :: Mouse HEXOKINASE 1 & 2 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS219117
Testing Data
Unit / Price
0.1 mg  /  $445 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HEXOKINASE 1 & 2 (HK2), Monoclonal Antibody

 Also Known As   

MOUSE ANTI HUMAN HEXOKINASE 1 & 2

 Product Gene Name   

anti-HK2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    601125
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    1A7
 Host    Mouse
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 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0 mg/ml (lot specific)
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 Perservative Stabilisers    0.1% Sodium Azide (NaN3)
Preparation
 Buffer Solution    Phosphate buffered saline
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-HK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-HK2 antibody

   Mouse anti Human Hexokinase 1&2, clone 1A7 recognizes human hexokinase 1 (HK1) and hexokinase 2 (HK2), 102kDa allosteric enzymes of the glycolytic pathway, which catalyse the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P). Hexokinase 1 is predominantly expressed in the brain while Hexokinase 2 is predominantly expressed in skeletal muscle. Both are members of four distinct mammalian hexokinase isozymes (hexokinase type I to IV), which localize to the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P (Wolf et al. 2011)
 Applications Tested/Suitable for anti-HK2 antibody   

ELISA (EIA), Western Blot (WB)

 Application Notes for anti-HK2 antibody    Western Blot: This item detects a band of approximately 102 kDa in Jurkat cell lysates.
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/3000
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 Testing Data of anti-HK2 antibody    Western blotting analysis of hexokinase specificity using Mouse anti Human hexokinase 1&2, clone 1A7 A: hexokinase specificity against recombinant hexokinase proteinsB: hexokinase expression in Jurkat cell lysate
anti-HK2 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for HK2. It may not necessarily be applicable to this product.
 NCBI GI #    15553127
 NCBI GeneID    3099
 NCBI Accession #    NP_000180.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000189.4 [Other Products]
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 UniProt Secondary Accession #    Q8WU87; Q9UN82; D6W5J2 [Other Products]
 UniProt Related Accession #    P52789 [Other Products]
 Molecular Weight    102,380 Da [Similar Products]
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 NCBI Official Full Name    hexokinase-2
 NCBI Official Synonym Full Names    hexokinase 2
 NCBI Official Symbol    HK2 [Similar Products]
 NCBI Official Synonym Symbols   
HKII; HXK2
[Similar Products]
 NCBI Protein Information    hexokinase-2; HK II; hexokinase type II; hexokinase-2, muscle; muscle form hexokinase
 UniProt Protein Name    Hexokinase-2
 UniProt Synonym Protein Names   
Hexokinase type II; HK II; Muscle form hexokinase
 Protein Family    Probable histidine kinase
 UniProt Gene Name    HK2 [Similar Products]
 UniProt Synonym Gene Names    HK II [Similar Products]
 UniProt Entry Name    HXK2_HUMAN
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 NCBI Summary for HK2    Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
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 UniProt Comments for HK2    HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.1; Kinase, other; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - fructose and mannose; Mitochondrial

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrial outer membrane; membrane; cytosol

Molecular Function: hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding; glucose binding

Biological Process: lactation; apoptotic mitochondrial changes; glycolysis; hexose transport; carbohydrate metabolic process; carbohydrate phosphorylation; glucose 6-phosphate metabolic process; cell glucose homeostasis; glucose metabolic process; pathogenesis; regulation of glucose import; glucose transport; transmembrane transport
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 Research Articles on HK2    1. High HK2 expression combined with low phosphorylated PDHA1 expression in the invasive front lesions of colorectal tumors is predictive of tumor aggressiveness and survival.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-HK2 antibodyPathways associated with anti-HK2 antibody
 Reference Product  PubMed Publications
 GPI antibody  >5 publications with HK2 and GPI
 PFKL antibody  >2 publications with HK2 and PFKL
 PFKP antibody  >2 publications with HK2 and PFKP
 PFKM antibody  >2 publications with HK2 and PFKM
 PGM1 antibody  >1 publications with HK2 and PGM1
 C12orf5 antibody  >1 publications with HK2 and C12orf5
 PFKFB3 antibody  >1 publications with HK2 and PFKFB3
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Butirosin And Neomycin Biosynthesis Pathway antibodies  Butirosin And Neomycin Biosynthesis Pathway Diagram
 Butirosin And Neomycin Biosynthesis Pathway antibodies  Butirosin And Neomycin Biosynthesis Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
Diseases associated with anti-HK2 antibodyOrgans/Tissues associated with anti-HK2 antibody
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >58 publications with HK2 and Kidney Diseases
 Hyperplasia Antibodies  >39 publications with HK2 and Hyperplasia
 Fibrosis Antibodies  >38 publications with HK2 and Fibrosis
 Death Antibodies  >29 publications with HK2 and Death
 Breast Neoplasms Antibodies  >25 publications with HK2 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >20 publications with HK2 and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >19 publications with HK2 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >18 publications with HK2 and Disease Models, Animal
 Inflammation Antibodies  >15 publications with HK2 and Inflammation
 Hypertrophy Antibodies  >11 publications with HK2 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >4 publications with HK2 and Ovary
 Placenta Antibodies  >4 publications with HK2 and Placenta
 Skin Antibodies  >3 publications with HK2 and Skin
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