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anti-HMOX1 antibody :: Mouse HO-1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS801987
Unit / Price
0.1 mg  /  $440 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HO-1 (HMOX1), Monoclonal Antibody

 Also Known As   

HO-1 Antibody: PerCP

 Product Synonym Names    Hsp32; HMOX1; Heme oxygenase 1; HO; HO1
 Product Gene Name   

anti-HMOX1 antibody

[Similar Products]
 Matching Pairs    Unconjugated Antibody: HO-1 Clone #1F12-A6 (MBS800054)
PerCP Conjugated Antibody: HO-1 Clone #1F12-A6 (MBS801987)
 Matching Pairs    Unconjugated Antibody: HO-1 Clone #1F12-A6 (MBS800757)
PerCP Conjugated Antibody: HO-1 Clone #1F12-A6 (MBS801987)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    gene 614034
 3D Structure    ModBase 3D Structure for P09601
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 Clonality    Monoclonal
 Isotype    IgG1 Kappa
 Clone Number    1F12-A6
 Host    Mouse
 Species Reactivity    Human, Mouse, Bovine
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 Form/Format    Protein G Purified
 Concentration    1mg/mL (lot specific)
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 Conjugate    PerCP
 Storage Buffer    PBS pH7.4, 50% glycerol, 0.09% sodium azide
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 Preparation and Storage    -20 degree C
 Other Notes    Small volumes of anti-HMOX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-HMOX1 antibody

   Background Info: Detects a 32kDa protein, corresponding to the molecular mass of HO-1 on SDS Page immunoblots. Does not cross-react with HO-2

Scientific Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
 Product Categories/Family for anti-HMOX1 antibody    Chaperones, Heat Shock, Organelle Markers, Oxidative Stress
 Applications Tested/Suitable for anti-HMOX1 antibody   

Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA (EIA)

 Application Notes for anti-HMOX1 antibody    1:500-1000 (WB)
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 Immunohistochemistry (IHC) of anti-HMOX1 antibody    Immunohistochemistry analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: muscle, dermis, hair follicles, epidermis: nuclear everywhere and some cytoplasmic staining.
anti-HMOX1 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-HMOX1 antibody    Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
anti-HMOX1 antibody Western Blot (WB) (WB) image
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 Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-HMOX1 antibody    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cell-cell border staining in epidermis, punctuate nuclear staining.
anti-HMOX1 antibody Immunocytochemistry/Immunofluorescence (ICC/IF) (ICC) image
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NCBI/Uniprot data below describe general gene information for HMOX1. It may not necessarily be applicable to this product.
 NCBI GI #    4504437
 NCBI GeneID    3162
 NCBI Accession #    NP_002124.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002133.2 [Other Products]
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 UniProt Primary Accession #    P09601 [Other Products]
 UniProt Related Accession #    P09601 [Other Products]
 Molecular Weight    32,819 Da [Similar Products]
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 NCBI Official Full Name    heme oxygenase 1
 NCBI Official Synonym Full Names    heme oxygenase (decycling) 1
 NCBI Official Symbol    HMOX1 [Similar Products]
 NCBI Official Synonym Symbols   
HO-1; HSP32; HMOX1D; bK286B10
[Similar Products]
 NCBI Protein Information    heme oxygenase 1; heat shock protein, 32-kD
 UniProt Protein Name    Heme oxygenase 1
 UniProt Gene Name    HMOX1 [Similar Products]
 UniProt Synonym Gene Names    HO; HO1; HO-1 [Similar Products]
 UniProt Entry Name    HMOX1_HUMAN
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 NCBI Summary for HMOX1    Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HMOX1    HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family.

Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; nucleolus; caveola; cytosol; nucleus

Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; metal ion binding; phospholipase D activity; heme binding; heme oxygenase (decyclizing) activity

Biological Process: response to nicotine; cell death; cellular iron ion homeostasis; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; negative regulation of mast cell degranulation; positive regulation of vasodilation; excretion; erythrocyte homeostasis; regulation of transcription factor activity; heme catabolic process; regulation of blood pressure; small GTPase mediated signal transduction; porphyrin metabolic process; negative regulation of mast cell cytokine production; angiogenesis; negative regulation of neuron apoptosis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; transmembrane transport; healing during inflammatory response; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; regulation of angiogenesis; negative regulation of leukocyte migration; negative regulation of DNA binding; positive regulation of angiogenesis; iron ion homeostasis; positive regulation of chemokine biosynthetic process; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to estrogen stimulus; endothelial cell proliferation; response to oxidative stress; smooth muscle hyperplasia

Disease: Heme Oxygenase 1 Deficiency; Pulmonary Disease, Chronic Obstructive
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Product References and Citations for anti-HMOX1 antibody

   1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411-419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925-10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759-1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129-135.
1. Al-Rifai, N., Rcker, H., Amslinger, S. (2013). Opening or Closing the Lock? When Reactivity Is the Key to Biological Activity. Chemistry - A Euro J. Article first published online: Sept 17, 2013. DOI: 10.1002/chem.201302117
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 Research Articles on HMOX1    1. Study shows that HO-1 drives insulin resistance in mouse and man; in matched biopsies from "healthy" versus insulin-resistant obese subjects we find HO-1 to be among the strongest positive predictors of metabolic disease in humans.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-HMOX1 antibodyPathways associated with anti-HMOX1 antibody
 Reference Product  PubMed Publications
 NFE2L2 antibody  >954 publications with HMOX1 and NFE2L2
 HIF1A antibody  >150 publications with HMOX1 and HIF1A
 NOS3 antibody  >66 publications with HMOX1 and NOS3
 VEGFA antibody  >37 publications with HMOX1 and VEGFA
 AKT1 antibody  >14 publications with HMOX1 and AKT1
 USF2 antibody  >5 publications with HMOX1 and USF2
 BLVRA antibody  >5 publications with HMOX1 and BLVRA
 FOSL1 antibody  >4 publications with HMOX1 and FOSL1
 SLC46A1 antibody  >2 publications with HMOX1 and SLC46A1
 Products by Pathway  Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Heme Degradation Pathway antibodies  Heme Degradation Pathway Diagram
 Iron Uptake And Transport Pathway antibodies  Iron Uptake And Transport Pathway Diagram
 Keap1-Nrf2 Pathway antibodies  Keap1-Nrf2 Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Porphyrins Pathway antibodies  Metabolism Of Porphyrins Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
Diseases associated with anti-HMOX1 antibodyOrgans/Tissues associated with anti-HMOX1 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1412 publications with HMOX1 and Inflammation
 Ischemia Antibodies  >819 publications with HMOX1 and Ischemia
 Reperfusion Injury Antibodies  >625 publications with HMOX1 and Reperfusion Injury
 Liver Diseases Antibodies  >439 publications with HMOX1 and Liver Diseases
 Hypertension Antibodies  >347 publications with HMOX1 and Hypertension
 Lung Injury Antibodies  >324 publications with HMOX1 and Lung Injury
 Myocardial Ischemia Antibodies  >264 publications with HMOX1 and Myocardial Ischemia
 Neurodegenerative Diseases Antibodies  >256 publications with HMOX1 and Neurodegenerative Diseases
 Fibrosis Antibodies  >216 publications with HMOX1 and Fibrosis
 Drug-Induced Liver Injury Antibodies  >201 publications with HMOX1 and Drug-Induced Liver Injury
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1900 publications with HMOX1 and Blood
 Vascular Antibodies  >1381 publications with HMOX1 and Vascular
 Liver Antibodies  >1127 publications with HMOX1 and Liver
 Brain Antibodies  >844 publications with HMOX1 and Brain
 Lung Antibodies  >761 publications with HMOX1 and Lung
 Kidney Antibodies  >748 publications with HMOX1 and Kidney
 Muscle Antibodies  >722 publications with HMOX1 and Muscle
 Heart Antibodies  >676 publications with HMOX1 and Heart
 Bone Antibodies  >210 publications with HMOX1 and Bone
 Skin Antibodies  >207 publications with HMOX1 and Skin
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