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anti-HPRG antibody :: Rat anti-Mouse HPRG Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS690930 anti-HPRG antibody
Unit / Price
0.1 mg  /  $505 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HPRG, Monoclonal Antibody

★Popular Item★
 Also Known As   

Rat Anti-Mouse HPRG

 Product Synonym Names    Anti-mouse Histidine and Proline Rich Glycoprotein [HPRG] (#9G42)
 Product Gene Name   

anti-HPRG antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 16; NC_000082.6 (22951072..22961659). Location: 16 B1; 16 13.79 cM
 3D Structure    ModBase 3D Structure for Q9ESB3
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    (9G42)
 Host    Rat
 Species Reactivity    Mouse
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 Specificity    This antibody detects mouse HPRG in Western blotting.
 Purity/Purification    Protein G/A affinity chromatography
 Form/Format    Lyophilized
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 Preparation    This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of HPRG. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
 Antigen    Recombinant mouse protein of HPRG
 Reconstitution    Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
 Reconstitution Buffer    PBS (sterile)
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 Preparation and Storage    Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
Ships at RT or 4 degree C
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-HPRG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-HPRG antibody

   Human histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. There are two N-terminal cystatin-like modules (aa 19-254) and one His-Pro-rich region (aa 350-497) that is flanked by two Pro-rich segments (aa 276-321 and 498-525). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N-and C-termini are linked by a disulfide bond. Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn ++dependent manner, it is converted to plasmin by tPA. HPRG also shows anti-angiogenic activity on vascular endothelial cells.
 Applications Tested/Suitable for anti-HPRG antibody   

Western Blot (WB)

 Application Notes for anti-HPRG antibody    Western blot: (1:500-1000)
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NCBI/Uniprot data below describe general gene information for HPRG. It may not necessarily be applicable to this product.
 NCBI GI #    226958456
 NCBI GeneID    94175
 NCBI Accession #    NP_444406.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_053176.2 [Other Products]
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 UniProt Primary Accession #    Q9ESB3 [Other Products]
 UniProt Secondary Accession #    Q6YK32; Q6YKA2; Q99PS5; Q99PS6 [Other Products]
 UniProt Related Accession #    Q9ESB3 [Other Products]
 Molecular Weight    59,163 Da
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 NCBI Official Full Name    histidine-rich glycoprotein
 NCBI Official Synonym Full Names    histidine-rich glycoprotein
 NCBI Official Symbol    Hrg [Similar Products]
 NCBI Official Synonym Symbols   
Hprg; Hrgp; D16jh2; D18020; AI265597; AW413091
[Similar Products]
 NCBI Protein Information    histidine-rich glycoprotein; histidine-proline-rich glycoprotein
 UniProt Protein Name    Histidine-rich glycoprotein
 UniProt Synonym Protein Names   
Histidine-proline-rich glycoprotein
 UniProt Gene Name    Hrg [Similar Products]
 UniProt Synonym Gene Names    HPRG [Similar Products]
 UniProt Entry Name    HRG_MOUSE
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 UniProt Comments for HPRG    Function: Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis

By similarity. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Ref.3 Ref.5 Ref.7 Ref.8 Ref.9

Cofactor: Zinc

By similarity.

Subunit structure: Interacts with THBS1 (via the TSP type I repeats); the interaction blocks the antiangiogenic effect of THBS1 with CD36. Interacts with HPSE; the interaction is enhanced at acidic pH, partially inhibits binding of HPSE to cell surface receptors and modulates its enzymatic activity. Interacts (via the HRR domain) with TMP1; the interaction partially mediates the antiangiogenic properties of HRG. Interacts with kappa and lambda light chains of IgG molecules. Interacts with ATP5A1; the interaction occurs on the surface of T-cells and alters their cell morphology in concert with CONA. Binds IgG molecules containing kappa and lambda light chains and inhibits the formation of insoluble immunoglobulin complexes. Interacts with F12; the interaction, which is enhanced in the presence of zinc ions and inhibited by heparin-binding to HRG, inhibits factor XII autoactivation and contact-initiated coagulation

By similarity. Interacts with PLG (via its Kringle domains); the interaction tethers PLG to the cell surface and enhances its activation. Interacts (via the HRR domain) with TPM1; the interaction appears to contribute to the antiangiogenic properties of the HRR domain

By similarity. Interacts with THBS2; the interaction blocks the antiangiogenic effect of THBS2 with CD36. Ref.5

Subcellular location: Secreted.

Tissue specificity: Expressed in liver, blood plasma, serum and in platelets. Also present in fibrin clots, wound fluid from acute wounds and chronic leg ulcers. Ref.1 Ref.7

Domain: The His-rich (HRR) region contains approximately 12 tandem internal repeats of the 5-residue G[H/P][H/P]PH consensus sequence. HRR binds heparan sulfate and possesses antiangiogenic, antibacterial and antifungal properties through binding Candida cells, and preferentially lysing the ergosterol-containing liposomes at low pH. The tandem repeats also bind divalent metal ions and heme.The cystatin domains can also bind heparan sulfate. Binding is enhanced in the presence of zinc ions

By similarity.

Post-translational modification: Proteolytic cleavage produces several HRG fragments which are mostly disulfide-linked and, therefore, not released. Cleavage by plasmin is inhibited in the presence of heparin, zinc ions or in an acidic environment. Cleavage reduces binding of HRG to heparan sulfate, but enhances the ability of HRG to bind and tether plasminogen to the cell surface. On platelet activation, releases a 33 kDa antiangiogenic peptide which encompasses the HRR. Also cleaved in the C-terminal by plasmin

By similarity.N-glycosylated.

Disruption phenotype: Null mice are viable and fertile, but have enhanced coagulation resulting in decreased bleeding times. The observed enhanced platelet activation mediates the accelerated angiogenic switch. Also enhanced fibrinolysis. Animals are unprotected against Candida fungal infection. Also show larger tumor volume in cancerous state, an excessive stimulation of tumor angiogenesis, a suppression of tumor immune respons and an increased tumor growth and metastatic spread. Ref.3 Ref.7 Ref.8 Ref.9 Ref.10

Sequence similarities: Contains 2 cystatin domains.

Sequence caution: The sequence AAN10183.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAN27996.1 differs from that shown. Reason: Erroneous gene model prediction.
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 Research Articles on HPRG    1. CD36-mediated anti-angiogenic activity has context-dependent role in the tumor microenvironment in the presence of TSP-1 while HRG modulates this activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-HPRG antibodyPathways associated with anti-HPRG antibody
 Reference Product  PubMed Publications
 Kng1 antibody  >5 publications with HPRG and Kng1
 Pf4 antibody  >2 publications with HPRG and Pf4
 Fn1 antibody  >2 publications with HPRG and Fn1
 Vwf antibody  >1 publications with HPRG and Vwf
 Serpinf2 antibody  >1 publications with HPRG and Serpinf2
 Products by Pathway  Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
Diseases associated with anti-HPRG antibodyOrgans/Tissues associated with anti-HPRG antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >197 publications with HPRG and Neoplasms
 Breast Neoplasms Antibodies  >129 publications with HPRG and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >36 publications with HPRG and Cardiovascular Diseases
 Immune System Diseases Antibodies  >33 publications with HPRG and Immune System Diseases
 Thrombosis Antibodies  >20 publications with HPRG and Thrombosis
 Nervous System Diseases Antibodies  >20 publications with HPRG and Nervous System Diseases
 Disease Models, Animal Antibodies  >18 publications with HPRG and Disease Models, Animal
 Adenocarcinoma Antibodies  >17 publications with HPRG and Adenocarcinoma
 Heart Diseases Antibodies  >15 publications with HPRG and Heart Diseases
 Necrosis Antibodies  >14 publications with HPRG and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >26 publications with HPRG and Muscle
 Liver Antibodies  >25 publications with HPRG and Liver
 Kidney Antibodies  >15 publications with HPRG and Kidney
 Lymph Node Antibodies  >3 publications with HPRG and Lymph Node
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