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anti-Hsf1 antibody :: Rat HSF1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS803679
Western Blot (WB)
Unit / Price
0.1 mg  /  $440 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HSF1, Monoclonal Antibody

 Also Known As   

HSF1 Antibody: ATTO 680

 Product Synonym Names    HSTF1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1
 Product Gene Name   

anti-Hsf1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P38532
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    10H4
 Host    Rat
 Species Reactivity    Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit
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 Form/Format    Protein G Purified
 Concentration    1mg/mL (lot specific)
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 Samples    Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit
 Storage Buffer    PBS pH 7.4, 50% glycerol, 0.1% sodium azide
 Conjugate    ATTO 680
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 Preparation and Storage    -20 degree C
 Other Notes    Small volumes of anti-Hsf1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Hsf1 antibody

   Background Info: Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.

Scientific Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
 Product Categories/Family for anti-Hsf1 antibody    Heat Shock
 Applications Tested/Suitable for anti-Hsf1 antibody   

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA (EIA), Gel Mobility Shift Assay

 Application Notes for anti-Hsf1 antibody    1:1000 WB
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 Western Blot (WB) of anti-Hsf1 antibody    Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:1000.
anti-Hsf1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for Hsf1. It may not necessarily be applicable to this product.
 NCBI GI #    33859480
 NCBI GeneID    15499
 NCBI Accession #    NP_032322.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008296.2 [Other Products]
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 UniProt Primary Accession #    P38532 [Other Products]
 UniProt Secondary Accession #    O70462 [Other Products]
 UniProt Related Accession #    P38532 [Other Products]
 Molecular Weight    54,931 Da [Similar Products]
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 NCBI Official Full Name    heat shock factor protein 1
 NCBI Official Synonym Full Names    heat shock factor 1
 NCBI Official Symbol    Hsf1 [Similar Products]
 NCBI Official Synonym Symbols   
AA960185; Hsf1beta; Hsf1alpha
[Similar Products]
 NCBI Protein Information    heat shock factor protein 1; HSF 1; HSTF 1; Hsf1gammabeta; Hsf1gammaalpha; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 gammabeta isoform; heat shock transcription factor 1 gammaalpha isoform
 UniProt Protein Name    Heat shock factor protein 1
 UniProt Synonym Protein Names   
Heat shock transcription factor 1; HSTF 1
 Protein Family    Heat shock factor protein
 UniProt Gene Name    Hsf1 [Similar Products]
 UniProt Synonym Gene Names    HSF 1; HSTF 1 [Similar Products]
 UniProt Entry Name    HSF1_MOUSE
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 UniProt Comments for Hsf1    HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; transcription, DNA-dependent; in utero embryonic development; embryonic process involved in female pregnancy; female meiosis; response to lipopolysaccharide; defense response; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; protein amino acid phosphorylation; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; response to heat; mRNA transcription; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis
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Product References and Citations for anti-Hsf1 antibody

   1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934. 2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82. 3. Tanaka K.I., et al. (2007). JBC Papers Online Manuscript M704081200. 4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796. 5. McMillan D. R., et al. (1998) J Bio Chem 273: 7523-7528. 6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774. 7. Fiorenza M.T., et al. (1995) Nucleic Acids Res. 23 (3):467-474. 8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293. 9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397. 10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334-1343. 11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.
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 Research Articles on Hsf1    1. These results, therefore, indicate that cellular stress can enhance osteoclast differentiation via Hsf1-dependent mechanisms and may significantly contribute to pathological and therapeutic related bone loss.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Hsf1 antibodyPathways associated with anti-Hsf1 antibody
 Reference Product  PubMed Publications
 Hsp72 antibody  >58 publications with Hsf1 and Hsp72
 Hspa1a antibody  >14 publications with Hsf1 and Hspa1a
 Mapk3 antibody  >10 publications with Hsf1 and Mapk3
 Dnajb1 antibody  >10 publications with Hsf1 and Dnajb1
 Il6 antibody  >7 publications with Hsf1 and Il6
 Rela antibody  >6 publications with Hsf1 and Rela
 Plk1 antibody  >5 publications with Hsf1 and Plk1
 Prkdc antibody  >4 publications with Hsf1 and Prkdc
 Smarca4 antibody  >3 publications with Hsf1 and Smarca4
 Hspa4 antibody  >2 publications with Hsf1 and Hspa4
 Products by Pathway  Pathway Diagram
 Attenuation Phase Pathway antibodies  Attenuation Phase Pathway Diagram
 Cellular Response To Heat Stress Pathway antibodies  Cellular Response To Heat Stress Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 HSF1 Activation Pathway antibodies  HSF1 Activation Pathway Diagram
 HSF1-dependent Transactivation Pathway antibodies  HSF1-dependent Transactivation Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Regulation Of HSF1-mediated Heat Shock Response Pathway antibodies  Regulation Of HSF1-mediated Heat Shock Response Pathway Diagram
Diseases associated with anti-Hsf1 antibodyOrgans/Tissues associated with anti-Hsf1 antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >65 publications with Hsf1 and Nervous System Diseases
 Inflammation Antibodies  >65 publications with Hsf1 and Inflammation
 Cardiovascular Diseases Antibodies  >46 publications with Hsf1 and Cardiovascular Diseases
 Brain Diseases Antibodies  >38 publications with Hsf1 and Brain Diseases
 Necrosis Antibodies  >38 publications with Hsf1 and Necrosis
 Breast Neoplasms Antibodies  >33 publications with Hsf1 and Breast Neoplasms
 Heart Diseases Antibodies  >26 publications with Hsf1 and Heart Diseases
 Cell Transformation, Neoplastic Antibodies  >22 publications with Hsf1 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >22 publications with Hsf1 and Liver Diseases
 Immune System Diseases Antibodies  >17 publications with Hsf1 and Immune System Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >101 publications with Hsf1 and Brain
 Muscle Antibodies  >76 publications with Hsf1 and Muscle
 Heart Antibodies  >69 publications with Hsf1 and Heart
 Liver Antibodies  >69 publications with Hsf1 and Liver
 Lung Antibodies  >34 publications with Hsf1 and Lung
 Testis Antibodies  >25 publications with Hsf1 and Testis
 Eye Antibodies  >23 publications with Hsf1 and Eye
 Embryonic Tissue Antibodies  >21 publications with Hsf1 and Embryonic Tissue
 Skin Antibodies  >18 publications with Hsf1 and Skin
 Stomach Antibodies  >12 publications with Hsf1 and Stomach
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