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anti-HCK antibody :: Rat anti-Human, Mouse Hck Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS632623 anti-HCK antibody
Unit / Price
0.1 mg  /  $605 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hck, Monoclonal Antibody

 Also Known As   

Hck (Hematopoietic Cellular Kinase)

 Product Synonym Names    Anti -Hck (Hematopoietic Cellular Kinase)
 Product Gene Name   

anti-HCK antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q95M30
 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    7H200
 Host    Rat
 Species Reactivity    Human, Mouse
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 Specificity    Detects endogenous human and mouse Hck at 59kD using Western Blot.
 Purity/Purification    Affinity Purified
Purified by Protein G affinity chromatography.
 Form/Format    Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
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 Immunogen    E. coli-derived rhHck (aa22-80)
 Hybridoma    Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived recombinant human Hck (rhHck; aa 22-80; accession # P08631).
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 Preparation and Storage    Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-HCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-HCK antibody

   Hemopoietic cell kinase (Hck) is a member of the Src family of tyrosine kinases. Hck expression is prominent in cells of myeloid lineage, particularly in granulocytes and monocytes, with protein levels increasing during terminal differentiation and macrophage activation. Hck contains a unique amino-terminal domain that is subject to lipid modifications, targeting this kinase to the plasma membrane.
 Product Categories/Family for anti-HCK antibody    Antibodies; Abs to Protein Kinases
 Applications Tested/Suitable for anti-HCK antibody   

Western Blot (WB)

 Application Notes for anti-HCK antibody    Western Blot: An antibody concentration of 1.0ug/ml is recommended., Optimal dilutions should be determined by each laboratory for each application.
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NCBI/Uniprot data below describe general gene information for HCK. It may not necessarily be applicable to this product.
 NCBI GI #    14627116
 NCBI Accession #    CAC44031.1 [Other Products]
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 UniProt Primary Accession #    Q95M30 [Other Products]
 Molecular Weight    57,096 Da [Similar Products]
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 NCBI Official Full Name    hck protein
 UniProt Protein Name    Tyrosine-protein kinase HCK
 UniProt Synonym Protein Names   
Hematopoietic cell kinase; Hemopoietic cell kinase; p56-HCK
 Protein Family    Tyrosine-protein kinase
 UniProt Gene Name    HCK [Similar Products]
 UniProt Entry Name    HCK_MACFA
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 UniProt Comments for HCK    Function: Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS

By similarity.

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation: Subject to autoinhibition, mediated by intramolecular interactions involving the SH2 and SH3 domains. Kinase activity is also regulated by phosphorylation at regulatory tyrosine residues. Phosphorylation at Tyr-389 is required for optimal activity. Phosphorylation at Tyr-500 inhibits kinase activity

By similarity.

Subunit structure: Interacts with ADAM15. Interacts with FASLG. Interacts with ARRB1 and ARRB2. Interacts with FCGR1A; the interaction may be indirect. Interacts with IL6ST. Interacts (via SH3 domain) with ELMO1. Interacts (via SH3 domain) with TP73. Interacts with YAP1. Interacts with ABL1 and ITGB1, and thereby recruits ABL1 to activated ITGB1. Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with CBL. Interacts with VAV1, WAS and RAPGEF1

By similarity.

Subcellular location: Cytoplasmic vesicle › secretory vesicle

By similarity. Cytoplasm › cytosol

By similarity. Cell membrane; Lipid-anchor. Membrane › caveola; Lipid-anchor

By similarity. Cell junction › focal adhesion

By similarity. Cytoplasm › cytoskeleton

By similarity. Golgi apparatus

By similarity. Cytoplasmic vesicle

By similarity. Lysosome

By similarity. Nucleus

By similarity. Note: A small fraction is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions

By similarity.

Post-translational modification: Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation at Tyr-389 increases kinase activity. Phosphorylation at Tyr-500 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-500, suggesting that this site may be a target of other kinases

By similarity.Ubiquitinated by CBL, leading to its degradation via the proteasome

By similarity.Palmitoylation requires prior myristoylation. Palmitoylation is required for caveolar localization

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.Contains 1 protein kinase domain.Contains 1 SH2 domain.Contains 1 SH3 domain.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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