View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Mouse His Tag Monoclonal Antibody Catalog # MBS525001 Unit / Price 0.05 mg / $230 +1 FREE 8GB USB 0.1 mg / $270 +1 FREE 8GB USB 0.2 mg / $355 +1 FREE 8GB USB 1 mg / $835 +1 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name His Tag, Monoclonal Antibody Popular Item Full Product Name Anti-His Tag (HIS.H8) Monoclonal Antibody Product Synonym Names Anti-His Tag Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG2b Clone Number HIS.H8 Host Mouse Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags) Purity/Purification Protein A affinity chromatography from mouse ascites fluid Form/Format 1 mg/ml in 10mM PBS, pH 7.2, with 0.05% NaN3 Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH Sensitivity WB (with ECL): 1-2ng of His-tagged fusion proteins Preparation and Storage Store at -20 degree C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4 degree C for several days to weeks. Other Notes Small volumes of anti-His Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Product Categories/Family for anti-His Tag antibody Epitope Tag mAbs Applications Tested/Suitable for anti-His Tag antibody Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB) Application Notes for anti-His Tag antibody WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature).IS: 1:500-2000 dilutionIP: 1:500-200 dilutionFor best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilutions by titration test Testing Data of anti-His Tag antibody Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue) Testing Data #2 of anti-His Tag antibody Fig. 02 Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL) Testing Data #3 of anti-His Tag antibody Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2) TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP