View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List anti-His antibody :: Mouse anti-other His Monoclonal Antibody Catalog # MBS415105 Unit / Price 0.1 mg / $290 +1 FREE 8GB USB 1 mg / $1,835 +2 FREE 8GB USB 5x1 mg / $7,985 +9 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name His, Monoclonal Antibody Popular Item Full Product Name Anti-His Antibody Product Gene Name anti-His antibody[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG2b Clone Number HIS.H8 Host Mouse Species Reactivity other Specificity His-tag fusion protein Purity/Purification IgG fraction Form/Format Liquid; PBS, pH 7.4 with 0.05% sodium azide. Concentration 100ug/100ul (lot specific) Cellular Localization Depends upon the localization of the parent protein tagged with hexahistidine. Tag Information Tag Immunogen 6x His synthetic peptide. Preparation and Storage This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Other Notes Small volumes of anti-His antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-His antibody The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Applications Tested/Suitable for anti-His antibody Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP) Application Notes for anti-His antibody WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. Other applications are optimized by end users. Western Blot of anti-His antibody Immunofluorescence of anti-His antibody TOP Protein Family Histone Product References and Citations for anti-His antibody Paluzzi, JP et al. "The heterodimeric glycoprotein hormone, GPA2, GPB5, regulates ion transport across the hindgut of the adult mosquito, Aedes aegypti." PLoS ONE 9:e86386 (2014). PubMed: 24466069. Application: Western Blot.Tsai, SL et al. "Functional display of complex cellulosomes on the yeast surface via adaptive assembly." ACS Synth Biol 2:14-21 (2013). PubMed: 23656322.Neilson, KM et al. "Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate." Dev Biol 365 (2):353-75 (2012). DOI: 10.1016/j.ydbio.2012.03.004. Application: Western Blot.Lim, KH et al. "Biotin-assisted folding of streptavidin on the yeast surface." Biotechnol prog 28 (1):276-283 (2012). DOI: 10.1002/btpr.721. PubMed: 21990063. Application: Immunoprecipitation.Bae, HJ et al. "DBC1 does not function as a negative regulator of SIRT1 in liver cancer." Oncol Lett 4 (5):873-877 (2012). PubMed: 23162614. Application: Western Blot.Liu, X et al. "Molecular and structural characterization of the SH3 domain of AHI1 in regulation of cellular resistance of BCRABL+ chronic myeloid leukemia cells to tyrosine." Proteomics 12 (13:2094-2106 (2012). DOI: 10.1002/pmic.201100553. Application: Western Blot.Park, UH et al. "Piperine, a Component of Black Pepper, Inhibits Adipogenesis by Antagonizing PPARgamma Activity in 3T3-L1 Cells." J Agric Food Chem 60 (15):3853-3860 (2012). DOI: 10.1021/jf204514a. PubMed: 22463744. Application: Western Blot.Olmstead, AD et al. "Human subtilase SKI-1, S1P is a master regulator of the HCV Lifecycle and a potential host cell target for developing indirect-acting antiviral agents." PLoS Pathog. 8:e1002468 (2012). PubMed: 22241994.Mani, T et al. "FERM domain phosphoinositide binding targets merlin to the membrane and is essential for its growth-suppressive function." Mol Cell Biol 31 (10):183-1996 (2011). DOI: 10.1128/MCB.00609-10. Application: Immunoblot.Xue, Y et al. "FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair." Hum. Mol. Genet. 17:1641-52 (2008). PubMed: 18285517.Metzler, M et al. "NMDA receptor function and NMDA receptor-dependent phosphorylation of huntingtin is altered by the endocytic protein HIP1." J Neurosci 27:2298-308 (2007). PubMed: 17329427. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP