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anti-h2ax antibody :: anti-Human, Mouse Histone H2A.X Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS475198
Unit / Price
0.1 mL  /  $335 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Histone H2A.X (h2ax), Monoclonal Antibody

 Also Known As   

Anti-Histone H2A.X (Phospho-Ser139) Mouse mAb

 Product Synonym Names    H2A histone family, member X; H2A.X; H2a/x; H2AFX; H2AX; H2AX histone; H2AX_HUMAN; Histone H2A.X; Histone H2AX
 Product Gene Name   

anti-h2ax antibody

[Similar Products]
 Product Synonym Gene Name    H2AX; H2A.X; H2A/X [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    X14850 mRNA
 3D Structure    ModBase 3D Structure for P16104
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 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    2A9-B5
 Host    Mouse
Source: Human
 Species Reactivity    Human, Mouse
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 Purity/Purification    Affinity Purified
 Form/Format    Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
 Concentration    4.2mg/ml (lot specific)
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 Immunogen    Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
 Sensitivity    This antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.
 Sequence Similarities    Belongs to the histone H2A family.
 Subunit Structure    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140 and MCPH1 when phosphorylated at Ser-140 or Tyr-143. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.
 Subcellular Location    Nucleus. Chromosome
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 Other Notes    Small volumes of anti-h2ax antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-h2ax antibody

   Entrez Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
 Product Categories/Family for anti-h2ax antibody    Epigenetics and Nuclear Signaling; Histones; H2A; Phosphorylated Epigenetics and Nuclear Signaling; Histones; H2A; Variants Kits/ Lysates/ Other; Kits; ELISA Kits; In Cell ELISA Kits; Phosphoprot
 Applications Tested/Suitable for anti-h2ax antibody   

Western Blot (WB), Immunofluorescence (IF)

 Application Notes for anti-h2ax antibody    WB: 1:2000
ICC: 1:400
Other: 1:400
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 Western Blot (WB) of anti-h2ax antibody    Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
anti-h2ax antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-h2ax antibody    Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X
anti-h2ax antibody Immunofluorescence (IF) image
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 Immunofluorescence (IF) of anti-h2ax antibody    Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549 (upper, untreated or Hydroxyurea-treated) and Hela (lower, untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).
anti-h2ax antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-h2ax antibody    Western blot analysis of extracts from untreated or Hydroxyurea-treated Hela and A549 cells, using Histone H2A.X (Phospho-Ser139) mouse mAb (1:1000 diluted) (upper) or beta-Actin Mouse mAb (200068-8F10) (lower).Predicted band size:15KDa.Observed band size:15KDa.
anti-h2ax antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for h2ax. It may not necessarily be applicable to this product.
 NCBI GI #    4504253
 NCBI GeneID    3014
 NCBI Accession #    NP_002096.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002105 [Other Products]
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 UniProt Primary Accession #    P16104 [Other Products]
 UniProt Secondary Accession #    Q4ZGJ7; Q6IAS5 [Other Products]
 UniProt Related Accession #    P16104 [Other Products]
 Molecular Weight    15
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 NCBI Official Full Name    histone H2AX
 NCBI Official Synonym Full Names    H2A histone family member X
 NCBI Official Symbol    H2AFX [Similar Products]
 NCBI Official Synonym Symbols   
H2AX; H2A.X; H2A/X
[Similar Products]
 NCBI Protein Information    histone H2AX
 UniProt Protein Name    Histone H2AX
 UniProt Synonym Protein Names   
Histone H2A.X
 UniProt Gene Name    H2AFX [Similar Products]
 UniProt Synonym Gene Names    H2AX; H2a/x [Similar Products]
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 NCBI Summary for h2ax    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
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 UniProt Comments for h2ax    H2AX: a histone that replaces conventional H2A in a subset of nucleosomes. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Phosphorylated on S139 by ATM and DNA-PK in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Dephosphorylation of S139 by PP2A is required for DNA DSB repair. Apparently phosphorylated on Y143 by WSTF, determining the relative recruitment of either DNA repair or pro-apoptotic factors. H2AXpY142 favors the recruitment of pro-apoptotic factors APBB1 and JNK1. In contrast, dephosphorylation of pY143 by EYA phosphatases favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated pS139. Monoubiquitination of K119 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'K63' linkages by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage.

Protein type: DNA repair, damage; DNA-binding; Helicase

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: chromosome, telomeric region; nuclear chromatin; nucleoplasm; nucleus

Molecular Function: DNA binding; enzyme binding; histone binding; protein binding

Biological Process: chromatin silencing; DNA damage checkpoint; double-strand break repair via nonhomologous end joining; response to DNA damage stimulus
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 Research Articles on h2ax    1. this study identified histone H2AX as an antigen of systemic lupus erythematosus by comparing highly ranked genes from all the built network-derived gene lists, which was confirmed the with real-world clinical samples
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-h2ax antibodyPathways associated with anti-h2ax antibody
 Reference Product  PubMed Publications
 ATM antibody  >283 publications with h2ax and ATM
 TP53BP1 antibody  >81 publications with h2ax and TP53BP1
 BRCA1 antibody  >73 publications with h2ax and BRCA1
 MDC1 antibody  >54 publications with h2ax and MDC1
 NBN antibody  >41 publications with h2ax and NBN
 MRE11A antibody  >41 publications with h2ax and MRE11A
 WRN antibody  >15 publications with h2ax and WRN
 Products by Pathway  Pathway Diagram
 ATM Mediated Phosphorylation Of Repair Proteins Pathway antibodies  ATM Mediated Phosphorylation Of Repair Proteins Pathway Diagram
 ATM Mediated Response To DNA Double-strand Break Pathway antibodies  ATM Mediated Response To DNA Double-strand Break Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Assembly Of The RAD50-MRE11-NBS1 Complex At DNA Double-strand Breaks Pathway antibodies  Assembly Of The RAD50-MRE11-NBS1 Complex At DNA Double-strand Breaks Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
Diseases associated with anti-h2ax antibodyOrgans/Tissues associated with anti-h2ax antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >446 publications with h2ax and Neoplasms
 Drug Toxicity Antibodies  >102 publications with h2ax and Drug Toxicity
 Breast Neoplasms Antibodies  >79 publications with h2ax and Breast Neoplasms
 Nervous System Diseases Antibodies  >54 publications with h2ax and Nervous System Diseases
 Gastrointestinal Diseases Antibodies  >53 publications with h2ax and Gastrointestinal Diseases
 Lung Neoplasms Antibodies  >49 publications with h2ax and Lung Neoplasms
 Adenocarcinoma Antibodies  >44 publications with h2ax and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >43 publications with h2ax and Neoplasms, Experimental
 Prostatic Neoplasms Antibodies  >25 publications with h2ax and Prostatic Neoplasms
 Cell Transformation, Neoplastic Antibodies  >24 publications with h2ax and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >135 publications with h2ax and Blood
 Lung Antibodies  >74 publications with h2ax and Lung
 Brain Antibodies  >37 publications with h2ax and Brain
 Skin Antibodies  >34 publications with h2ax and Skin
 Prostate Antibodies  >33 publications with h2ax and Prostate
 Bone Antibodies  >27 publications with h2ax and Bone
 Liver Antibodies  >21 publications with h2ax and Liver
 Vascular Antibodies  >17 publications with h2ax and Vascular
 Heart Antibodies  >14 publications with h2ax and Heart
 Mouth Antibodies  >14 publications with h2ax and Mouth
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