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anti-H3 antibody :: Rabbit Histone H3, methyl, pan Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS604457 anti-H3 antibody
Unit / Price
0.04 mL  /  $370 +1 FREE 8GB USB
0.1 mL  /  $550 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Histone H3, methyl, pan, Monoclonal Antibody

 Also Known As   

Histone H3, methyl, pan (Lys9)

 Product Synonym Names    Anti -Histone H3, methyl, pan (Lys9)
 Product Gene Name   

anti-H3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    10E31
 Host    Rabbit
 Species Reactivity    Human, Monkey, Mouse, Rat
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 Specificity    Recognizes human pan-methyl Histone H3 (Lys9) (D54). Species crossreactivity
 Form/Format    Supplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and 0.02% sodium azide.
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 Immunogen    Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is di-methylated (KLH-coupled).
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-H3 antibody

   The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1) and WD-40 domains (WDR5) (5-8). The recent discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic mark (9).
 Product Categories/Family for anti-H3 antibody    Antibodies; Abs to Histones
 Applications Tested/Suitable for anti-H3 antibody   

Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

 Application Notes for anti-H3 antibody    Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence (IF-IC) and Immunoprecipitation (Chromatin).
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Immunofluorescence (IF-IC) 1:50
Chromatin Immunoprecipitation (CHIP): 1:25
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NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.
 NCBI GI #    47551065
 NCBI GeneID    373335
 NCBI Accession #    NP_999709.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_214544.1 [Other Products]
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 NCBI Official Full Name    histone H3
 NCBI Official Symbol    H3 [Similar Products]
 NCBI Protein Information    histone H3
 Protein Family    Tyrosine phosphatase
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 Research Articles on H3    1. PHD domain of the only known invertebrate homolog of RAG2, the SpRAG2L protein of the purple sea urchin (Strongylocentrotus purpuratus) also binds to methylated histones, but with a unique preference for H3K4me2
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Organs/Tissues associated with anti-H3 antibody
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with H3 and Bone
 Connective Tissue Antibodies  >4 publications with H3 and Connective Tissue
 Heart Antibodies  >3 publications with H3 and Heart
 Muscle Antibodies  >3 publications with H3 and Muscle
 Brain Antibodies  >3 publications with H3 and Brain
 Vascular Antibodies  >2 publications with H3 and Vascular
 Embryonic Tissue Antibodies  >2 publications with H3 and Embryonic Tissue
 Ear Antibodies  >1 publications with H3 and Ear
 Kidney Antibodies  >1 publications with H3 and Kidney
 Umbilical Cord Antibodies  >1 publications with H3 and Umbilical Cord
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