View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Mouse Histones, pan Monoclonal Antibody Catalog # MBS600046 Unit / Price 0.1 mg / $1,015 +1 FREE 8GB USB 5x0.1 mg / $4,400 +5 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Histones, pan, Monoclonal Antibody Popular Item Full Product Name Histones, pan Product Synonym Names Anti -Histones, pan Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG1 Clone Number 2Q2283 Host Mouse Species Reactivity bovine, rat, Xenopusm opossum, hamster. Specificity Recognizes human histones HI1, H2A, h2B, H3 and H4 with comparable sensitivity. Binds specifically to an antigen determinant, which is present on all five histone proteins. Purity/Purification Affinity PurifiedPurified by Protein A affinity chromatography. Form/Format Supplied as a liquid in 0.02M PBS, pH 7.6, 0.09% sodium azide. Concentration 1 mg/mL (lot specific) Immunogen Purified nuclear fractions of HeLa cells Preparation and Storage May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Other Notes Small volumes of anti-Histones, pan antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-Histones, pan antibody There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail histones are involved with the structure of the nucleosomes of the 'beads on a string' structure. Product Categories/Family for anti-Histones, pan antibody Antibodies; Abs to Histones Applications Tested/Suitable for anti-Histones, pan antibody Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested. Application Notes for anti-Histones, pan antibody Immunohistochemistry: 1-5 ug/mLWestern Blot: Detects a band ~20kDOptimal dilutions to be determined by the researcher. Western Blot (WB) of anti-Histones, pan antibody Western Blot analysis in Jurkat lysate (Lane 1) & mouse brain lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with H3505 (1:500).|Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.|Arrow indicates protein Histone (Pan) (~20kD). Western Blot (WB) of anti-Histones, pan antibody Western Blot analysis in Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS600046 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.|Arrow indicates protein Histone (Pan) (~20kD). Immunofluorescence (IF) of anti-Histones, pan antibody Confocal fluorescent analysis of NIH/3T3 using MBS600046 mouse mAB(blue). Actin filaments have been labeled with Rhodamine Phalloidin(red). TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP