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anti-Hsp27 antibody :: Mouse anti-Human Hsp27 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200046
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hsp27, Monoclonal Antibody

 Also Known As   

Hsp27 antibody

 Product Synonym Names    Heat shock 27 kDa protein; Hspb1; Hsp25; Hsp27; Hsp27; HspB1; Heat shock 27 kDa protein; HSP 27; Growth-related 25 kDa protein; P25; HSP25; Heat shock protein beta-1; Heat shock 27 kDa protein 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; at shock 25kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat shock protein beta 1; Heat Shock Protein27; HS.76067; Hsp 28; Hsp B1; Hsp28; HspB1; SRP27; Stress responsive protein 27.
 Product Gene Name   

anti-Hsp27 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    L39370 Genomic DNA
 3D Structure    ModBase 3D Structure for P04792
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 Clonality    Monoclonal
 Isotype    IgG1, k
 Clone Number    2A5
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Immunogen    Recombinant human Hsp27 (1-205aa) purified from E. coli
 Clone Note    Anti-human Hsp27 mAb, clone 2A5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp27 protein.
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 Preparation and Storage    Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-Hsp27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Hsp27 antibody

   Hsp27 (also known as the Estrogen-Regulated 24k protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPkAP kinase 2 and the stress-activated protein kinase SAPk2 (p38). Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state.
 Product Categories/Family for anti-Hsp27 antibody    Heat Shock Proteins
 Applications Tested/Suitable for anti-Hsp27 antibody   

ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

 Application Notes for anti-Hsp27 antibody    Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.
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 Western Blot (WB) of anti-Hsp27 antibody    The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: K562 cell lysate Lane 3.: MCF7 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A431 cell lysate Lane 2.: HepG2 cell lysate
anti-Hsp27 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-Hsp27 antibody    Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
anti-Hsp27 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-Hsp27 antibody    ICC/IF analysis of Hsp27 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Hsp27 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-Hsp27 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for Hsp27. It may not necessarily be applicable to this product.
 NCBI GI #    4504517
 NCBI GeneID    3315
 NCBI Accession #    NP_001531 [Other Products]
 NCBI GenBank Nucleotide #    NM_001540.4 [Other Products]
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 UniProt Primary Accession #    P04792 [Other Products]
 UniProt Secondary Accession #    Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8 [Other Products]
 UniProt Related Accession #    P04792 [Other Products]
 Molecular Weight    22,783 Da
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 NCBI Official Full Name    heat shock protein beta-1
 NCBI Official Synonym Full Names    heat shock protein family B (small) member 1
 NCBI Official Symbol    HSPB1 [Similar Products]
 NCBI Official Synonym Symbols   
CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
[Similar Products]
 NCBI Protein Information    heat shock protein beta-1
 UniProt Protein Name    Heat shock protein beta-1
 UniProt Synonym Protein Names   
28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
 Protein Family    Heat shock protein
 UniProt Gene Name    HSPB1 [Similar Products]
 UniProt Synonym Gene Names    HSP27; HSP28; HspB1; HSP 27; SRP27 [Similar Products]
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 NCBI Summary for Hsp27    This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
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 UniProt Comments for Hsp27    Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
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Product References and Citations for anti-Hsp27 antibody

   Ehrnsperger M, et al., (1997) EMBO J 16:221-229; Steve J, et al., (2000) Mol Cell Biol 20 (20):7602-7612; Butt E, et al., (2001) J Biol Chem 276:7108-7113; Michael, et al., (2002) Cell Stress Chsperones 7 (3):281-296
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 Research Articles on Hsp27    1. found knock down of HSPB1 further increased the proportion of apoptotic cells in hyperthermic treated melanoma cells when compared with either single agent alone, and both agents leaded to cell cycle arrest at G0/G1 or G2/M phases
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Hsp27 antibodyPathways associated with anti-Hsp27 antibody
 Reference Product  PubMed Publications
 MAPKAPK2 antibody  >59 publications with Hsp27 and MAPKAPK2
 HSPA1A antibody  >29 publications with Hsp27 and HSPA1A
 HSPA8 antibody  >22 publications with Hsp27 and HSPA8
 MAPK14 antibody  >7 publications with Hsp27 and MAPK14
 MAPKAPK5 antibody  >5 publications with Hsp27 and MAPKAPK5
 MAPKAPK3 antibody  >4 publications with Hsp27 and MAPKAPK3
 ESR1 antibody  >2 publications with Hsp27 and ESR1
 EIF4G1 antibody  >1 publications with Hsp27 and EIF4G1
 Products by Pathway  Pathway Diagram
 ATM Signaling Network In Development And Disease Pathway antibodies  ATM Signaling Network In Development And Disease Pathway Diagram
 AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway antibodies  AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway antibodies  Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Fas Ligand (FasL) Pathway And Stress Induction Of Heat Shock Proteins (HSP) Regulation antibodies  Fas Ligand (FasL) Pathway And Stress Induction Of Heat Shock Proteins (HSP) Regulation Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
Diseases associated with anti-Hsp27 antibodyOrgans/Tissues associated with anti-Hsp27 antibody
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >114 publications with Hsp27 and Disease Models, Animal
 Inflammation Antibodies  >85 publications with Hsp27 and Inflammation
 Necrosis Antibodies  >83 publications with Hsp27 and Necrosis
 Heart Diseases Antibodies  >66 publications with Hsp27 and Heart Diseases
 Liver Diseases Antibodies  >51 publications with Hsp27 and Liver Diseases
 Lung Neoplasms Antibodies  >43 publications with Hsp27 and Lung Neoplasms
 Reperfusion Injury Antibodies  >42 publications with Hsp27 and Reperfusion Injury
 Kidney Diseases Antibodies  >38 publications with Hsp27 and Kidney Diseases
 Liver Neoplasms Antibodies  >33 publications with Hsp27 and Liver Neoplasms
 Carcinoma, Squamous Cell Antibodies  >32 publications with Hsp27 and Carcinoma, Squamous Cell
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >263 publications with Hsp27 and Muscle
 Blood Antibodies  >207 publications with Hsp27 and Blood
 Brain Antibodies  >199 publications with Hsp27 and Brain
 Heart Antibodies  >134 publications with Hsp27 and Heart
 Nerve Antibodies  >124 publications with Hsp27 and Nerve
 Vascular Antibodies  >115 publications with Hsp27 and Vascular
 Lung Antibodies  >95 publications with Hsp27 and Lung
 Liver Antibodies  >94 publications with Hsp27 and Liver
 Kidney Antibodies  >81 publications with Hsp27 and Kidney
 Eye Antibodies  >56 publications with Hsp27 and Eye
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