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anti-Hsp27 antibody :: Mouse anti-Human Hsp27 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200046
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hsp27, Monoclonal Antibody

 Also Known As   

Hsp27 antibody (clone 2A5)

 Product Synonym Names    Hspb1; Hsp25; Hsp27; Hsp27; HspB1; Heat shock 27 kDa protein; HSP 27; Growth-related 25 kDa protein; P25; HSP25; Heat shock protein beta-1; Heat shock 27 kDa protein 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; at shock 25kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat shock protein beta 1; Heat Shock Protein27; HS.76067; Hsp 28; Hsp B1; Hsp28; HspB1; SRP27; Stress responsive protein 27.
 Product Gene Name   

anti-Hsp27 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG1, k
 Clone Number    2A5
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Clone    Anti-human Hsp27 mAb, clone 2A5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp27 protein.
 Immunogen    Recombinant human Hsp27 (1-205aa) purified from E Coli
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 Preparation and Storage    Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-Hsp27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-Hsp27 antibody

   Hsp27 (also known as the Estrogen-Regulated 24k protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPkAP kinase 2 and the stress-activated protein kinase SAPk2 (p38). Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state.
 Product Categories/Family for anti-Hsp27 antibody    Heat Shock Proteins
 Applications Tested/Suitable for anti-Hsp27 antibody   

ELISA (EIA), Western Blot (WB) (Cell lysate), Immunohistochemistry (IHC)

 Application Notes for anti-Hsp27 antibody    The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.
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 Immunohistochemistry (IHC) of anti-Hsp27 antibody    Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
anti-Hsp27 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-Hsp27 antibody    Cell lysates of HeLa(each 30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-Hsp27 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for Hsp27. It may not necessarily be applicable to this product.
 NCBI GI #    4504517
 NCBI GeneID    3315
 NCBI Accession #    NP_001531 [Other Products]
 NCBI GenBank Nucleotide #    NM_001540.3 [Other Products]
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 UniProt Secondary Accession #    Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8 [Other Products]
 UniProt Related Accession #    P04792 [Other Products]
 Molecular Weight    22,783 Da
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 NCBI Official Full Name    heat shock protein beta-1
 NCBI Official Synonym Full Names    heat shock 27kDa protein 1
 NCBI Official Symbol    HSPB1 [Similar Products]
 NCBI Official Synonym Symbols   
CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
[Similar Products]
 NCBI Protein Information    heat shock protein beta-1; 28 kDa heat shock protein; HSP 27; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27
 UniProt Protein Name    Heat shock protein beta-1
 UniProt Synonym Protein Names   
28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
 Protein Family    Heat shock protein
 UniProt Gene Name    HSPB1 [Similar Products]
 UniProt Synonym Gene Names    HSP27; HSP28; HspB1; HSP 27; SRP27 [Similar Products]
 UniProt Entry Name    HSPB1_HUMAN
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 NCBI Summary for Hsp27    The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
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 UniProt Comments for Hsp27    HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Protein type: Heat shock protein; Motility/polarity/chemotaxis; Chaperone

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; nucleus; cytosol; Z disc

Molecular Function: identical protein binding; protein kinase C inhibitor activity; ubiquitin binding; protein binding; protein kinase C binding; protein kinase binding

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; response to virus; positive regulation of blood vessel endothelial cell migration; response to unfolded protein; retinal homeostasis; positive regulation of interleukin-1 beta production; positive regulation of angiogenesis; negative regulation of protein kinase activity; gene expression; vascular endothelial growth factor receptor signaling pathway; cell motility; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of translational initiation; negative regulation of apoptosis

Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f
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Product References and Citations for anti-Hsp27 antibody

   Ehrnsperger M, et al., (1997) EMBO J 16:221-229 Steve J, et al., (2000) Mol Cell Biol 20(20):7602-7612 Butt E, et al., (2001) J Biol Chem 276:7108-7113 Michael, et al., (2002) Cell Stress Chsperones 7(3):281-296
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 Research Articles on Hsp27    1. This study demonstrates that HSP27 phosphorylation serves as a novel regulator in TNF-alpha-induced apoptosis, and provides a new insight into the cytoprotective role of HSP27 phosphorylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-Hsp27 antibodyDiseases associated with anti-Hsp27 antibody
 Products by Pathway  Pathway Diagram
 AUF1 (hnRNP D0) Destabilizes MRNA Pathway antibodies  AUF1 (hnRNP D0) Destabilizes MRNA Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >121 publications with Hsp27 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >96 publications with Hsp27 and Disease Models, Animal
 Necrosis Antibodies  >70 publications with Hsp27 and Necrosis
 Inflammation Antibodies  >67 publications with Hsp27 and Inflammation
 Breast Neoplasms Antibodies  >62 publications with Hsp27 and Breast Neoplasms
 Heart Diseases Antibodies  >60 publications with Hsp27 and Heart Diseases
 Liver Diseases Antibodies  >42 publications with Hsp27 and Liver Diseases
 Reperfusion Injury Antibodies  >35 publications with Hsp27 and Reperfusion Injury
 Kidney Diseases Antibodies  >32 publications with Hsp27 and Kidney Diseases
 Lung Neoplasms Antibodies  >31 publications with Hsp27 and Lung Neoplasms
Organs/Tissues associated with anti-Hsp27 antibody
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >229 publications with Hsp27 and Muscle
 Blood Antibodies  >156 publications with Hsp27 and Blood
 Brain Antibodies  >154 publications with Hsp27 and Brain
 Heart Antibodies  >115 publications with Hsp27 and Heart
 Nerve Antibodies  >109 publications with Hsp27 and Nerve
 Vascular Antibodies  >97 publications with Hsp27 and Vascular
 Liver Antibodies  >81 publications with Hsp27 and Liver
 Lung Antibodies  >73 publications with Hsp27 and Lung
 Kidney Antibodies  >72 publications with Hsp27 and Kidney
 Skin Antibodies  >44 publications with Hsp27 and Skin
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