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anti-Hsp60 antibody :: Rabbit Hsp60 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS179619
Western Blot (WB)
Unit / Price
0.1 mg  /  $280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hsp60, Monoclonal Antibody

 Also Known As   

Anti-Hsp60 Rabbit Monoclonal Antibody

 Product Synonym Names    60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; HSPD1; HSP60
 Product Gene Name   

anti-Hsp60 antibody

[Similar Products]
 Product Synonym Gene Name    HSPD1 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    M22382 mRNA
 3D Structure    ModBase 3D Structure for P10809
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 Clonality    Monoclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Affinity-chromatography
 Form/Format    Liquid
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 Immunogen    A synthesized peptide derived from human Hsp60
 Subcellular Localization    Mitochondrion matrix.
 Contents    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Preparation and Storage    Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-Hsp60 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Hsp60 antibody

   Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
 Product Categories/Family for anti-Hsp60 antibody    Tags & Cell Markers; Subcellular Markers; organelles; mitochondria; Signal Transduction; Protein Trafficking; chaperones; heat shock proteins; Kits/Lysates/Other; ELISA Kits; signal transduction proteins ELISA Kits; isotype/loading controls; hsp60
 Applications Tested/Suitable for anti-Hsp60 antibody   

Immunohistochemistry (IHC), Western Blot (WB)

 Application Notes for anti-Hsp60 antibody    WB: 1:500-1:2000
IHC: 1:50-1:200
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 Western Blot (WB) of anti-Hsp60 antibody    Western blot analysis of Hsp60 expression in HeLa cell lysate (MBS179619).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPD1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPD1
anti-Hsp60 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for Hsp60. It may not necessarily be applicable to this product.
 NCBI GI #    31542947
 NCBI GeneID    3329
 NCBI Accession #    NP_002147.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002156.4 [Other Products]
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 UniProt Primary Accession #    P10809 [Other Products]
 UniProt Secondary Accession #    Q38L19; Q9UCR6; B2R5M6; B7Z712 [Other Products]
 UniProt Related Accession #    P10809 [Other Products]
 Molecular Weight    61055 MW
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 NCBI Official Full Name    60 kDa heat shock protein, mitochondrial
 NCBI Official Synonym Full Names    heat shock protein family D (Hsp60) member 1
 NCBI Official Symbol    HSPD1 [Similar Products]
 NCBI Official Synonym Symbols   
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
[Similar Products]
 NCBI Protein Information    60 kDa heat shock protein, mitochondrial
 UniProt Protein Name    60 kDa heat shock protein, mitochondrial
 UniProt Synonym Protein Names   
60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
 Protein Family    Hydrogenosomal chaperonin
 UniProt Gene Name    HSPD1 [Similar Products]
 UniProt Synonym Gene Names    HSP60; CPN60; HSP-60; Hsp60 [Similar Products]
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 NCBI Summary for Hsp60    This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
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 UniProt Comments for Hsp60    Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
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 Research Articles on Hsp60    1. high level of ROS is needed for tumorigenesis and progression in tumors with low HSP60 expression
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Hsp60 antibodyPathways associated with anti-Hsp60 antibody
 Reference Product  PubMed Publications
 HSPE1 antibody  >8 publications with Hsp60 and HSPE1
 CASP3 antibody  >3 publications with Hsp60 and CASP3
 PPP2R1B antibody  >1 publications with Hsp60 and PPP2R1B
 PPP2R1A antibody  >1 publications with Hsp60 and PPP2R1A
 BAK1 antibody  >1 publications with Hsp60 and BAK1
 ALDH2 antibody  >1 publications with Hsp60 and ALDH2
 PRNP antibody  >1 publications with Hsp60 and PRNP
 Products by Pathway  Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Mitochondrial Protein Import Pathway antibodies  Mitochondrial Protein Import Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Type I Diabetes Mellitus Pathway antibodies  Type I Diabetes Mellitus Pathway Diagram
Diseases associated with anti-Hsp60 antibodyOrgans/Tissues associated with anti-Hsp60 antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >17 publications with Hsp60 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >10 publications with Hsp60 and Cardiovascular Diseases
 Inflammation Antibodies  >9 publications with Hsp60 and Inflammation
 Carcinoma Antibodies  >8 publications with Hsp60 and Carcinoma
 Brain Diseases Antibodies  >8 publications with Hsp60 and Brain Diseases
 Hyperplasia Antibodies  >4 publications with Hsp60 and Hyperplasia
 Atherosclerosis Antibodies  >4 publications with Hsp60 and Atherosclerosis
 Lung Diseases Antibodies  >4 publications with Hsp60 and Lung Diseases
 Necrosis Antibodies  >3 publications with Hsp60 and Necrosis
 Lung Neoplasms Antibodies  >3 publications with Hsp60 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11 publications with Hsp60 and Blood
 Brain Antibodies  >7 publications with Hsp60 and Brain
 Liver Antibodies  >5 publications with Hsp60 and Liver
 Vascular Antibodies  >5 publications with Hsp60 and Vascular
 Testis Antibodies  >4 publications with Hsp60 and Testis
 Heart Antibodies  >4 publications with Hsp60 and Heart
 Muscle Antibodies  >4 publications with Hsp60 and Muscle
 Lung Antibodies  >3 publications with Hsp60 and Lung
 Embryonic Tissue Antibodies  >3 publications with Hsp60 and Embryonic Tissue
 Bone Antibodies  >3 publications with Hsp60 and Bone
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