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anti-HIF1A antibody :: Mouse anti-Human Hypoxia Inducible Factor 1a Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS601822 anti-HIF1A antibody
Unit / Price
0.1 mg  /  $570 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hypoxia Inducible Factor 1a (HIF1A), Monoclonal Antibody

 Also Known As   

Hypoxia Inducible Factor 1a (HIF-1a) (BSA & Azide Free)

 Product Synonym Names    Anti -Hypoxia Inducible Factor 1a (HIF-1a) (BSA & Azide Free)
 Product Gene Name   

anti-HIF1A antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 14; NC_000014.8 (62162119..62214977). Location: 14q23.2
 OMIM    603348
 3D Structure    ModBase 3D Structure for Q16665
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 Clonality    Monoclonal
 Isotype    IgG1,k
 Clone Number    0.N.327
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Recognizes the alpha subunit of human hypoxia-inducible factor-1 (HIF-1) at 120kD.
 Purity/Purification    Affinity Purified
Purified by Protein G affinity chromatography.
 Form/Format    Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins and no preservatives added. Also available with BSA and azide. See H9805.
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 Immunogen    Recombinant protein corresponding to aa530-826 of human HIF-1a.
 Cellular Localization    Nuclear
 Positive Control    Mammalian cells cultured under reduced O2 tension.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-HIF1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-HIF1A antibody

   HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF-1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions, and iron chelation. HIF-1 activates transcription of hypoxia-inducible genes, including those encoding: erythropoietin, vascular endothelial growth (VEGF), heme oxygenase-1, inducible nitric oxide synthase, and the glycolytic enzymes aldolase A, enolase 1, lactate dehydrogenase A, phosphofructokinase I and phosphoglycerate kinase I. The c-terminal of HIR-1 alpha binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF.
 Product Categories/Family for anti-HIF1A antibody    Antibodies; Abs to Transcription Factors
 Applications Tested/Suitable for anti-HIF1A antibody   

Immunofluorescence (IF)

 Application Notes for anti-HIF1A antibody    Suitable for use in Immunofluorescence and Gel Supershift. Not suitable for use in Western Blot.
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NCBI/Uniprot data below describe general gene information for HIF1A. It may not necessarily be applicable to this product.
 NCBI GI #    15214788
 NCBI GeneID    3091
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 UniProt Primary Accession #    Q16665 [Other Products]
 UniProt Secondary Accession #    Q53XP6; Q96PT9; Q9UPB1 [Other Products]
 UniProt Related Accession #    Q16665 [Other Products]
 Molecular Weight    92,670 Da [Similar Products]
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 NCBI Official Full Name    Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 NCBI Official Synonym Full Names    hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 NCBI Official Symbol    HIF1A [Similar Products]
 NCBI Official Synonym Symbols   
HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 1-alpha; HIF-1-alpha; OTTHUMP00000179061; OTTHUMP00000179062; OTTHUMP00000179063; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 UniProt Protein Name    Hypoxia-inducible factor 1-alpha
 UniProt Synonym Protein Names   
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8
 Protein Family    Hypoxia-inducible factor
 UniProt Gene Name    HIF1A [Similar Products]
 UniProt Synonym Gene Names    BHLHE78; MOP1; PASD8 [Similar Products]
 UniProt Entry Name    HIF1A_HUMAN
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 NCBI Summary for HIF1A    This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]
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 UniProt Comments for HIF1A    HIF1A: a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia- mimicking conditions. Interacts with USP20. Interacts with RACK1; promotes HIF1A ubiquitination and proteasome- mediated degradation. Interacts (via N-terminus) with USP19. Under reduced oxygen tension. Induced also by various receptor-mediated factors such as growth factors, cytokines, and circulatory factors such as PDGF, EGF, FGF2, IGF2, TGFB1, HGF, TNF, IL1B, angiotensin-2 and thrombin. However, this induction is less intense than that stimulated by hypoxia. Repressed by HIPK2 and LIMD1. Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Autophagy; DNA-binding

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nuclear speck; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone deacetylase binding; Hsp90 protein binding; protein kinase binding; transcription factor binding; histone acetyltransferase binding; signal transducer activity; protein binding; enzyme binding; sequence-specific DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription factor activity; nuclear hormone receptor binding

Biological Process: lactation; oxygen homeostasis; embryonic placenta development; response to muscle activity; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; signal transduction; glucose homeostasis; positive regulation of vascular endothelial growth factor receptor signaling pathway; muscle maintenance; negative regulation of bone mineralization; elastin metabolic process; connective tissue replacement during inflammatory response; axon transport of mitochondrion; regulation of transcription, DNA-dependent; visual learning; angiogenesis; heart looping; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; neural crest cell migration; negative regulation of growth; hemoglobin biosynthetic process; positive regulation of neuroblast proliferation; negative regulation of TOR signaling pathway; regulation of transforming growth factor-beta2 production; Notch signaling pathway; collagen metabolic process; embryonic hemopoiesis; positive regulation of erythrocyte differentiation; positive regulation of nitric-oxide synthase activity; B-1 B cell homeostasis; digestive tract morphogenesis; mRNA transcription from RNA polymerase II promoter; positive regulation of chemokine production; positive regulation of angiogenesis; neural fold elevation formation; regulation of gene expression; positive regulation of hormone biosynthetic process; cartilage development; positive regulation of glycolysis; lactate metabolic process; response to hypoxia; epithelial to mesenchymal transition; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development
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 Research Articles on HIF1A    1. Hypoxia inducible factor-1alpha has a role in human emphysema lung tissue
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Hypoxia Inducible Factor 1a (HIF-1a) (BSA & Azide Free)
 Catalog #  Product Name  Catalog #  Product Name
 MBS601822  Hypoxia Inducible Factor 1a (HIF-1a) (BSA & Azide Free)  MBS602200  Anti - Hypoxia Inducible Factor 1a (HIF-1a)
 MBS603203  Hypoxia Inducible Factor 1a (HIF-1a) (BSA & Azide Free)  MBS602974  Hypoxia Inducible Factor 1a (HIF-1a)
 MBS395106  Hif-1a (hypoxia inducible factor 1a)  MBS602852  Hypoxia Inducible Factor 1a (HIF-1a)
 MBS600429  Hypoxia Inducible Factor 1a (HIF-1a)  MBS606669  Hypoxia Inducible Factor 1a (HIF-1a)
 MBS614010  Hypoxia Inducible Factor 1a (HIF-1a)  MBS602520  Hypoxia Inducible Factor 1a (HIF-1a)
Products associated with anti-HIF1A antibodyPathways associated with anti-HIF1A antibody
 Reference Product  PubMed Publications
 VHL antibody  >436 publications with HIF1A and VHL
 VEGFA antibody  >331 publications with HIF1A and VEGFA
 ARNT antibody  >250 publications with HIF1A and ARNT
 EPO antibody  >216 publications with HIF1A and EPO
 EGLN1 antibody  >97 publications with HIF1A and EGLN1
 JUN antibody  >83 publications with HIF1A and JUN
 MAPK3 antibody  >72 publications with HIF1A and MAPK3
 STAT3 antibody  >65 publications with HIF1A and STAT3
 EGLN3 antibody  >53 publications with HIF1A and EGLN3
 HIF1AN antibody  >50 publications with HIF1A and HIF1AN
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Hypoxic And Oxygen Homeostasis Regulation Of HIF-1-alpha Pathway antibodies  Hypoxic And Oxygen Homeostasis Regulation Of HIF-1-alpha Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
 PDGFR-beta Signaling Pathway antibodies  PDGFR-beta Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram
 Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway antibodies  Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway Diagram
Diseases associated with anti-HIF1A antibodyOrgans/Tissues associated with anti-HIF1A antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2151 publications with HIF1A and Neoplasms
 Neovascularization, Pathologic Antibodies  >632 publications with HIF1A and Neovascularization, Pathologic
 Cardiovascular Diseases Antibodies  >566 publications with HIF1A and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >467 publications with HIF1A and Adenocarcinoma
 Inflammation Antibodies  >278 publications with HIF1A and Inflammation
 Breast Neoplasms Antibodies  >269 publications with HIF1A and Breast Neoplasms
 Kidney Diseases Antibodies  >261 publications with HIF1A and Kidney Diseases
 Lung Diseases Antibodies  >233 publications with HIF1A and Lung Diseases
 Liver Diseases Antibodies  >231 publications with HIF1A and Liver Diseases
 Neoplasm Metastasis Antibodies  >205 publications with HIF1A and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >2234 publications with HIF1A and Vascular
 Blood Antibodies  >1242 publications with HIF1A and Blood
 Muscle Antibodies  >470 publications with HIF1A and Muscle
 Brain Antibodies  >459 publications with HIF1A and Brain
 Kidney Antibodies  >434 publications with HIF1A and Kidney
 Liver Antibodies  >372 publications with HIF1A and Liver
 Lung Antibodies  >363 publications with HIF1A and Lung
 Heart Antibodies  >347 publications with HIF1A and Heart
 Bone Antibodies  >233 publications with HIF1A and Bone
 Embryonic Tissue Antibodies  >203 publications with HIF1A and Embryonic Tissue
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