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anti-IDH1 R132H antibody :: Mouse anti-Human IDH1 R132H Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS346014
Immunohistochemistry (IHC)
Unit / Price
0.01 mL  /  $170 +1 FREE 8GB USB
0.1 mL  /  $410 +1 FREE 8GB USB
0.2 mL  /  $580 +1 FREE 8GB USB
0.5 mL  /  $920 +1 FREE 8GB USB
1 L  /  $1,355 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IDH1 R132H, Monoclonal Antibody

 Also Known As   

IDH1 R132H Antibody

 Product Gene Name   

anti-IDH1 R132H antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    IHC132
 Host    Mouse
 Species Reactivity    Human
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 Form/Format    Liquid
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 Buffer    Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
 IHC Positive Control    Astrocytoma
 IHC Suggested Protocol    1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab Dilute antibodies 1:100 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)
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 Preparation and Storage    Store at 2-8 degree C. Do not freeze.
 Other Notes    Small volumes of anti-IDH1 R132H antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-IDH1 R132H antibody

   IDH1 (Isocitrate Dehydrogenase 1) is a cytosolic enzyme that catalyzes the decarboxylation of isocitrate in the TCA metabolic cycle. The mutation R132H is clinically indicated in the majority of astrocytomas and oligodendroglial tumors, favoring positive prognosis and increased survival in patients.
 Applications Tested/Suitable for anti-IDH1 R132H antibody   

Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-IDH1 R132H antibody    Dilution Range: 1:100 - 1:200
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 Immunohistochemistry (IHC) of anti-IDH1 R132H antibody    IHC (paraffin) of human astrocytoma using IDH1 R132H Antibody
anti-IDH1 R132H antibody Immunohistochemistry (IHC) (IHC) image
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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