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anti-IL3 antibody :: Rat anti-Human IL-3 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS670035 anti-IL3 antibody
Unit / Price
0.5 mg  /  $295 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IL-3 (IL3), Monoclonal Antibody

 Also Known As   

Rat Anti-Human IL-3-UNLB

 Product Synonym Names    Rat Anti-Human IL-3 - Purified, Unlabeled Antibody; Rat Anti-Human IL-3
 Product Gene Name   

anti-IL3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 5; NC_000005.9 (131396347..131398896). Location: 5q31.1
 OMIM    147740
 3D Structure    ModBase 3D Structure for P08700
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 Clonality    Monoclonal
 Isotype    Rat IgG1, kappa
 Clone Number    BVD3-1F9
 Host    Rat
 Species Reactivity    Human
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 Specificity    Human Interleukin-3 (IL-3)1-4
 Form/Format    Purified (UNLB) Antibody
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 Immunogen    Yeast-expressed recombinant human IL-3
 Characterization    To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
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 Preparation and Storage    The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
 Other Notes    Small volumes of anti-IL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-IL3 antibody   

ELISA; Flow Cytometry; Immunofluorescence

 Application Notes for anti-IL3 antibody    Research Application(s): ELISA Detection: BVD3-1F9 monoclonal antibody is the preferred detection antibody in a sandwich ELISA for quantifying human IL-3 protein levels.1-3 Biotinylated BVD3-1F9 antibody should be paired with purified BVD8-3G11 antibody as the capture antibody, with purified recombinant human IL-3 as the standard.

Immunofluorescence/Flow Cytometry: BVD3-1F9 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-3-positive cells within mixed cell populations. Recombinant human IL-3, unlabeled BVD3-1F9, or Biotinylated Rat IgG1 should be used as a specificity control.

Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
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NCBI/Uniprot data below describe general gene information for IL3. It may not necessarily be applicable to this product.
 NCBI GI #    3002475
 NCBI GeneID    3562
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 UniProt Primary Accession #    P08700 [Other Products]
 UniProt Secondary Accession #    Q6GS87 [Other Products]
 UniProt Related Accession #    P08700; Q6NZ78; Q6NZ79 [Other Products]
 Molecular Weight    17,233 Da [Similar Products]
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 NCBI Official Full Name    IL-3
 NCBI Official Synonym Full Names    interleukin 3 (colony-stimulating factor, multiple)
 NCBI Official Symbol    IL3 [Similar Products]
 NCBI Official Synonym Symbols   
IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF
[Similar Products]
 NCBI Protein Information    interleukin-3; OTTHUMP00000065963; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor
 UniProt Protein Name    Interleukin-3
 UniProt Synonym Protein Names   
Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
 Protein Family    Interleukin
 UniProt Gene Name    IL3 [Similar Products]
 UniProt Entry Name    IL3_HUMAN
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 NCBI Summary for IL3    The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq]
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 UniProt Comments for IL3    Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Tissue specificity: Activated T-cells, mast cells, natural killer cells.

Sequence similarities: Belongs to the IL-3 family.
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Product References and Citations for anti-IL3 antibody

   1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Abrams, J., J. Silver, R. van Dyke, and G. Gleich. 1994. Eosinophil-active cytokines in human disease: Development and use of monoclonal antibodies to IL-3-, IL-5, GM-CSF. In Eosinophils in Allergy and Inflammation (A. Kay and G. Gleich, eds). Marcel Dekker, New York, pp. 133-157.
3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
4. Kaushansky, K. 1992. The structure-function relationships of interleukin-3: An analysis based on the function and binding characteristics of a series of interspecies chimera of gibbon and murine IL-3. J. Clin. Invest. 90:1879-1888.
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 Research Articles on IL3    1. The IL3 genotypes rs40401 and rs40401 were found to exert a protective effect against malaria attacks.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-IL3 antibodyPathways associated with anti-IL3 antibody
 Reference Product  PubMed Publications
 CSF2RB antibody  >10 publications with IL3 and CSF2RB
 IL3RA antibody  >6 publications with IL3 and IL3RA
 PIK3CD antibody  >1 publications with IL3 and PIK3CD
 PIK3CA antibody  >1 publications with IL3 and PIK3CA
 HRAS antibody  >1 publications with IL3 and HRAS
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
Diseases associated with anti-IL3 antibodyOrgans/Tissues associated with anti-IL3 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >115 publications with IL3 and Neoplasms
 Leukemia Antibodies  >99 publications with IL3 and Leukemia
 Leukemia, Myeloid Antibodies  >67 publications with IL3 and Leukemia, Myeloid
 Hematologic Diseases Antibodies  >47 publications with IL3 and Hematologic Diseases
 Leukemia, Myeloid, Acute Antibodies  >39 publications with IL3 and Leukemia, Myeloid, Acute
 Bone Marrow Diseases Antibodies  >35 publications with IL3 and Bone Marrow Diseases
 Hypersensitivity Antibodies  >21 publications with IL3 and Hypersensitivity
 Lung Diseases Antibodies  >18 publications with IL3 and Lung Diseases
 Neoplasms, Experimental Antibodies  >18 publications with IL3 and Neoplasms, Experimental
 Drug Toxicity Antibodies  >17 publications with IL3 and Drug Toxicity
 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >11 publications with IL3 and Mixed
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