|
| Product Name
|
|
IL12, Monoclonal Antibody
|
| Also Known As
|
|
IL12 antibody
|
| Product Synonym Names
|
|
Monoclonal IL12; Anti-IL12; CTL maturation factor; Cytotoxic lymphocyte maturation factor; Interleukin 12; TSF; IL-12; CLMF; TCMF; NKSF; IL 12; IL12
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
|
| OMIM
|
|
601604
|
| 3D Structure
|
|
ModBase 3D Structure for P42701
|
|
| Clonality
|
|
Monoclonal
|
| Isotype
|
|
IgG1 kappa
|
| Clone Number
|
|
M12
|
| Host
|
|
Mouse
|
| Species Reactivity
|
|
Human IL-12
|
|
|
| Form/Format
|
|
Lyophilized from PBS.
|
|
|
| Biological Significance
|
|
IL12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells.
|
| Immunogen
|
|
IL12 antibody was raised in mouse using highly pure recombinant human IL-12 as the immunogen.
|
|
|
| Preparation and Storage
|
|
Store at -20 degree C until reconstitution. Following reconstitution product may be stored at 4 degree C in the short term. For long term storage aliquot and freeze at -20 degree C. Avoid repeated freeze/thaw cycles.
|
| Other Notes
|
|
Small volumes of anti-IL12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
|
TOP
|
|
|
|
|
Mouse monoclonal IL12 antibody
|
| Product Categories/Family for anti-IL12 antibody
|
|
Cytokines & Growth Factors
|
|
| Applications Tested/Suitable for anti-IL12 antibody
|
|
ELISA (EIA), Inhibition, Western Blot (WB)
|
| Application Notes for anti-IL12 antibody
|
|
ELISA: 2-4 ug/ml
WB: 0.5-1 ug/ml
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for IL12. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
5031785
|
| NCBI GeneID
|
|
3594
|
| NCBI Accession #
|
|
NP_005526.1
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_005535.1
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
P42701
|
[Other Products]
|
| UniProt Secondary Accession #
|
|
Q8N6Q7; A8K308; B2RPF1; B7ZKK3
|
[Other Products]
|
| UniProt Related Accession #
|
|
P42701
|
[Other Products]
|
| Molecular Weight
|
|
75 kDa (predicted detection band MW)
|
|
TOP
|
| NCBI Official Full Name
|
|
interleukin-12 receptor subunit beta-1 isoform 1
|
| NCBI Official Synonym Full Names
|
|
interleukin 12 receptor, beta 1
|
| NCBI Official Symbol
|
|
IL12RB1
|
[Similar Products]
|
| NCBI Official Synonym Symbols
|
|
CD212; IMD30; IL12RB; IL-12R-BETA1
|
[Similar Products]
|
| NCBI Protein Information
|
|
interleukin-12 receptor subunit beta-1
|
| UniProt Protein Name
|
|
Interleukin-12 receptor subunit beta-1
|
| UniProt Synonym Protein Names
|
|
IL-12 receptor beta component; CD_antigen: CD212
|
| UniProt Gene Name
|
|
IL12RB1
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
IL12R; IL12RB; IL-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12R-beta-1; IL-12RB1
|
[Similar Products]
|
| UniProt Entry Name
|
|
I12R1_HUMAN
|
|
|
| NCBI Summary for IL12
|
|
The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
|
|
|
| UniProt Comments for IL12
|
|
IL12RB1: Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade. Defects in IL12RB1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Belongs to the type I cytokine receptor family. Type 2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 19p13.1
Cellular Component: external side of plasma membrane
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-12 receptor activity; interleukin-23 binding; interleukin-23 receptor activity; interleukin-12 receptor binding
Biological Process: positive regulation of memory T cell differentiation; positive regulation of interferon-gamma production; peptidyl-tyrosine phosphorylation; positive regulation of T-helper 1 type immune response; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; signal transduction; positive regulation of defense response to virus by host; positive regulation of activated T cell proliferation
Disease: Immunodeficiency 30
|
|
TOP
|
|
| Research Articles on IL12
|
|
1. Genetic variations of IL-12B, IL-12Rbeta1, IL-12Rbeta2 in Behcet's disease and VKH syndrome.
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with anti-IL12 antibody | Pathways associated with anti-IL12 antibody |
|
|
|
| Diseases associated with anti-IL12 antibody | Organs/Tissues associated with anti-IL12 antibody |
|
|
|
|
|
|
TOP
|
Antibody 
