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anti-IL33 antibody :: Mouse anti-Human IL33 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200087
Western Blot (WB)
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IL33, Monoclonal Antibody

 Also Known As   

IL33 antibody (clone 4E9)

 Product Synonym Names    Interleukin 33; DVS27; NF-HEV; NKHEV; C9orf26; IL-33; Interleukin 33 CHROMOSOME 9 OPEN READING FRAME 26; DKFZp586H0523; DVS27 related protein; IL 1F11; IL 33; IL1F11; Interleukin 1 family member 11; INTERLEuKIN 33 NFHEV; Interleukin 33 precursor; Interleukin33; NF HEV; NFHEV; Nuclear factor from high endothelial venules; RP11 575C20.2; il33
 Product Gene Name   

anti-IL33 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG2b, k
 Clone Number    4E9
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Clone    Anti-human IL-33 mAb, clone 4E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL-33 protein.
 Immunogen    Recombinant human IL-33 (112-270aa) purified from E Coli
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 Preparation and Storage    Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-IL33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-IL33 antibody

   Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
 Product Categories/Family for anti-IL33 antibody    Hormones & Cytokines
 Applications Tested/Suitable for anti-IL33 antibody   

ELISA (EIA), Western Blot (WB) (Cell lysate)

 Application Notes for anti-IL33 antibody    The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.
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 Western Blot (WB) of anti-IL33 antibody    The Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-IL33 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for IL33. It may not necessarily be applicable to this product.
 NCBI GI #    15559209
 NCBI GeneID    90865
 NCBI Accession #    NP_254274 [Other Products]
 NCBI GenBank Nucleotide #    NM_033439.3 [Other Products]
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 UniProt Secondary Accession #    Q2YEJ5; B2R8L1; B4DJ35; B4E1Q9; D3DRI5; E7EAX4 [Other Products]
 UniProt Related Accession #    O95760 [Other Products]
 Molecular Weight    16,250 Da
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 NCBI Official Full Name    interleukin-33 isoform 1
 NCBI Official Synonym Full Names    interleukin 33
 NCBI Official Symbol    IL33 [Similar Products]
 NCBI Official Synonym Symbols   
DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
[Similar Products]
 NCBI Protein Information    interleukin-33; DVS27-related protein; IL-1F11; IL-33; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules
 UniProt Protein Name    Interleukin-33
 UniProt Synonym Protein Names   
Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV
 Protein Family    Interleukin
 UniProt Gene Name    IL33 [Similar Products]
 UniProt Synonym Gene Names    C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV [Similar Products]
 UniProt Entry Name    IL33_HUMAN
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 NCBI Summary for IL33    IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]
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 UniProt Comments for IL33    IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular space; transport vesicle; chromosome; nucleus

Molecular Function: protein binding; cytokine activity

Biological Process: positive regulation of T-helper 2 type immune response; negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response
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Product References and Citations for anti-IL33 antibody

   Brint Ek, et al., (2005) J Biol Chem. 20:277(51) Schmitz, et al., (2005) immunity. 23:479 Baekkevold ES, et al., (2003) Am.J.Pathol. 163(1):69-90
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 Research Articles on IL33    1. IL-33 is involved in u-PA-driven angiogenesis, a process that has been shown before to be linked to inflammation in various pathologies.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-IL33 antibodyPathways associated with anti-IL33 antibody
 Reference Product  PubMed Publications
 IL1RL1 antibody  >158 publications with IL33 and IL1RL1
 TSLP antibody  >19 publications with IL33 and TSLP
 CASP1 antibody  >17 publications with IL33 and CASP1
 IL18 antibody  >16 publications with IL33 and IL18
 IL25 antibody  >14 publications with IL33 and IL25
 IL18R1 antibody  >7 publications with IL33 and IL18R1
 VEGFA antibody  >3 publications with IL33 and VEGFA
 IL1B antibody  >2 publications with IL33 and IL1B
 CSF2 antibody  >1 publications with IL33 and CSF2
 Products by Pathway  Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
Diseases associated with anti-IL33 antibodyOrgans/Tissues associated with anti-IL33 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >186 publications with IL33 and Inflammation
 Asthma Antibodies  >86 publications with IL33 and Asthma
 Hypersensitivity, Immediate Antibodies  >82 publications with IL33 and Hypersensitivity, Immediate
 Lung Diseases Antibodies  >82 publications with IL33 and Lung Diseases
 Necrosis Antibodies  >55 publications with IL33 and Necrosis
 Cardiovascular Diseases Antibodies  >50 publications with IL33 and Cardiovascular Diseases
 Heart Diseases Antibodies  >30 publications with IL33 and Heart Diseases
 Fibrosis Antibodies  >20 publications with IL33 and Fibrosis
 Dermatitis, Atopic Antibodies  >18 publications with IL33 and Dermatitis, Atopic
 Disease Models, Animal Antibodies  >18 publications with IL33 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >53 publications with IL33 and Lung
 Skin Antibodies  >42 publications with IL33 and Skin
 Heart Antibodies  >42 publications with IL33 and Heart
 Vascular Antibodies  >33 publications with IL33 and Vascular
 Muscle Antibodies  >19 publications with IL33 and Muscle
 Intestine Antibodies  >17 publications with IL33 and Intestine
 Liver Antibodies  >16 publications with IL33 and Liver
 Brain Antibodies  >12 publications with IL33 and Brain
 Eye Antibodies  >9 publications with IL33 and Eye
 Connective Tissue Antibodies  >7 publications with IL33 and Connective Tissue
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