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anti-ISG15 antibody :: Mouse anti-Human ISG15 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200045
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ISG15, Monoclonal Antibody

★Popular Item★
 Also Known As   

ISG15 antibody

 Product Synonym Names    Interferon-stimulated gene 15 kDa ubiquitin-like modifier; GIP2; uCRP; IFI15,ISG15,Interferon-stimulated gene; 15 kDa ubiquitin-like modifier; Interferon-stimulated gene; 15 kDa ubiquitin-like modifier IFI 15; Interferon alpha inducible protein; Interferon induced 15 kDa protein; Interferon induced 17 kDa protein; Interferon induced 17 kDa/15 kDa protein; Interferon Stimulated Gene 15; Interferon stimulated protein 15 kDa; ISG 15; ISG15 ubiquitin like modifier; ubiquitin cross reactive protein precursor.
 Product Gene Name   

anti-ISG15 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    M13755 mRNA
 3D Structure    ModBase 3D Structure for P05161
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 Clonality    Monoclonal
 Isotype    IgG1, k
 Clone Number    3E5
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Immunogen    Recombinant human ISG15 (1-157aa) purified from E. coli
 Clone Note    Anti-human ISG15 mAb, clone 3E5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ISG15 protein.
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 Preparation and Storage    Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-ISG15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ISG15 antibody

   ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide.
 Product Categories/Family for anti-ISG15 antibody    Ubiquitination
 Applications Tested/Suitable for anti-ISG15 antibody   

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

 Application Notes for anti-ISG15 antibody    WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.
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 Western Blot (WB) of anti-ISG15 antibody    The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate
anti-ISG15 antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-ISG15 antibody    ICC/IF analysis of ISG15 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ISG15 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-ISG15 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for ISG15. It may not necessarily be applicable to this product.
 NCBI GI #    4826774
 NCBI GeneID    9636
 NCBI Accession #    NP_005092 [Other Products]
 NCBI GenBank Nucleotide #    NM_005101.3 [Other Products]
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 UniProt Primary Accession #    P05161 [Other Products]
 UniProt Secondary Accession #    Q5SVA4; Q7Z2G2; Q96GF0 [Other Products]
 UniProt Related Accession #    P05161 [Other Products]
 Molecular Weight    17,888 Da
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 NCBI Official Full Name    ubiquitin-like protein ISG15
 NCBI Official Synonym Full Names    ISG15 ubiquitin-like modifier
 NCBI Official Symbol    ISG15 [Similar Products]
 NCBI Official Synonym Symbols   
G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
[Similar Products]
 NCBI Protein Information    ubiquitin-like protein ISG15
 UniProt Protein Name    Ubiquitin-like protein ISG15
 UniProt Synonym Protein Names   
Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP
 Protein Family    Ubiquitin-like protein
 UniProt Gene Name    ISG15 [Similar Products]
 UniProt Synonym Gene Names    G1P2; UCRP; IP17; hUCRP [Similar Products]
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 NCBI Summary for ISG15    The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]
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 UniProt Comments for ISG15    Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Can also isgylate: EIF2AK2/PKR which results in its activation, DDX58/RIG-I which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade therby inhibiting IFNA-induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity.
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Product References and Citations for anti-ISG15 antibody

   Jonathan D et al., (1996) Proc Natl Acad Sci USA 93:211-215; Weiming Y et al., (2001) EMBO J 20 (3):362-371; Chen Z et al., (2005) Proc Natl Acad Sci USA 102 (29):10200-10205
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 Research Articles on ISG15    1. findings revealed that MERS-CoV PLpro chiefly engages human ISG15 through its C-terminal domain; structure of MERS-CoV PLpro was solved to 2.4 A in complex with the C-terminal domain of hISG15; structure of MERS-CoV PLpro in complex with this domain exposed the interface between these two entities
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ISG15 antibodyPathways associated with anti-ISG15 antibody
 Reference Product  PubMed Publications
 MX1 antibody  >97 publications with ISG15 and MX1
 USP18 antibody  >87 publications with ISG15 and USP18
 OAS1 antibody  >44 publications with ISG15 and OAS1
 HERC5 antibody  >40 publications with ISG15 and HERC5
 IFIT1 antibody  >39 publications with ISG15 and IFIT1
 DDX58 antibody  >22 publications with ISG15 and DDX58
 IFIT3 antibody  >15 publications with ISG15 and IFIT3
 IFI6 antibody  >14 publications with ISG15 and IFI6
 IFIT2 antibody  >14 publications with ISG15 and IFIT2
 UBA7 antibody  >6 publications with ISG15 and UBA7
 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DNA Damage Bypass Pathway antibodies  DNA Damage Bypass Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Host Interactions With Influenza Factors Pathway antibodies  Host Interactions With Influenza Factors Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Infectious Disease Pathway antibodies  Infectious Disease Pathway Diagram
 Influenza Infection Pathway antibodies  Influenza Infection Pathway Diagram
Diseases associated with anti-ISG15 antibodyOrgans/Tissues associated with anti-ISG15 antibody
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >76 publications with ISG15 and Liver Diseases
 Inflammation Antibodies  >58 publications with ISG15 and Inflammation
 Influenza, Human Antibodies  >38 publications with ISG15 and Influenza, Human
 Skin Diseases Antibodies  >36 publications with ISG15 and Skin Diseases
 Necrosis Antibodies  >35 publications with ISG15 and Necrosis
 Kidney Diseases Antibodies  >28 publications with ISG15 and Kidney Diseases
 Disease Models, Animal Antibodies  >28 publications with ISG15 and Disease Models, Animal
 Fever Antibodies  >22 publications with ISG15 and Fever
 Lung Diseases Antibodies  >19 publications with ISG15 and Lung Diseases
 Breast Neoplasms Antibodies  >17 publications with ISG15 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >184 publications with ISG15 and Blood
 Liver Antibodies  >82 publications with ISG15 and Liver
 Kidney Antibodies  >51 publications with ISG15 and Kidney
 Lung Antibodies  >46 publications with ISG15 and Lung
 Brain Antibodies  >45 publications with ISG15 and Brain
 Uterus Antibodies  >41 publications with ISG15 and Uterus
 Bone Antibodies  >21 publications with ISG15 and Bone
 Skin Antibodies  >17 publications with ISG15 and Skin
 Placenta Antibodies  >15 publications with ISG15 and Placenta
 Intestine Antibodies  >13 publications with ISG15 and Intestine
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