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anti-IRF3 antibody :: Mouse anti-Human Interferon regulatory factor-3 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200059
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interferon regulatory factor-3 (IRF3), Monoclonal Antibody

 Also Known As   

Interferon regulatory factor-3 antibody

 Product Synonym Names    IRF3; Interferon regulatory factor-3; IRF-3; Interferon regulatory factor 3; IRF 3.
 Product Gene Name   

anti-IRF3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    Z56281 mRNA
 3D Structure    ModBase 3D Structure for Q14653
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 Clonality    Monoclonal
 Isotype    IgG1, k
 Clone Number    3F10
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Immunogen    Recombinant human IRF-3 (108-166aa) purified from E. coli
 Clone Note    Anti-human IRF-3 mAb, clone 3F10, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IRF-3 protein.
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 Preparation and Storage    Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-IRF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-IRF3 antibody

   Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection.
 Product Categories/Family for anti-IRF3 antibody    Hormones & Cytokines
 Applications Tested/Suitable for anti-IRF3 antibody   

ELISA (EIA), WB (Cell lysate), Immunoprecipitation (IP), Immunofluorescence (IF)

 Application Notes for anti-IRF3 antibody    WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.
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 Western Blot (WB) of anti-IRF3 antibody    Cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-IRF3 antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-IRF3 antibody    Immunofluorescence of human HeLa cells stained with IRF-3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.
anti-IRF3 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for IRF3. It may not necessarily be applicable to this product.
 NCBI GI #    4504725
 NCBI GeneID    3661
 NCBI Accession #    NP_001562 [Other Products]
 NCBI GenBank Nucleotide #    NM_001571.5 [Other Products]
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 UniProt Primary Accession #    Q14653 [Other Products]
 UniProt Secondary Accession #    Q5FBY1; Q5FBY2; Q5FBY4; Q7Z5G6; A8K7L2; B2RAZ3 [Other Products]
 UniProt Related Accession #    Q14653 [Other Products]
 Molecular Weight    12,330 Da [Similar Products]
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 NCBI Official Full Name    interferon regulatory factor 3 isoform 1
 NCBI Official Synonym Full Names    interferon regulatory factor 3
 NCBI Official Symbol    IRF3 [Similar Products]
 NCBI Official Synonym Symbols   
IIAE7
[Similar Products]
 NCBI Protein Information    interferon regulatory factor 3
 UniProt Protein Name    Interferon regulatory factor 3
 Protein Family    Interferon regulatory factor
 UniProt Gene Name    IRF3 [Similar Products]
 UniProt Synonym Gene Names    IRF-3 [Similar Products]
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 NCBI Summary for IRF3    This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
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 UniProt Comments for IRF3    Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
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Product References and Citations for anti-IRF3 antibody

   DeFilippis VR et al., (2006) J Virol 80 (2):1032-1037; Pietila TE et al., (2007) J Immunol 178 (1):253-261
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 Research Articles on IRF3    1. New research suggests that altering a subset of extracellular matrix factors, including interferon regulatory factor (IRF)3 and casein kinase (CK)2, may decrease the migratory potential of these aggressive tumors.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-IRF3 antibodyPathways associated with anti-IRF3 antibody
 Reference Product  PubMed Publications
 TBK1 antibody  >377 publications with IRF3 and TBK1
 TLR4 antibody  >295 publications with IRF3 and TLR4
 MAVS antibody  >164 publications with IRF3 and MAVS
 DDX58 antibody  >148 publications with IRF3 and DDX58
 TICAM1 antibody  >71 publications with IRF3 and TICAM1
 TRAF3 antibody  >61 publications with IRF3 and TRAF3
 IKBKE antibody  >29 publications with IRF3 and IKBKE
 IFNB1 antibody  >26 publications with IRF3 and IFNB1
 CREBBP antibody  >17 publications with IRF3 and CREBBP
 TMEM173 antibody  >8 publications with IRF3 and TMEM173
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies  Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
Diseases associated with anti-IRF3 antibodyOrgans/Tissues associated with anti-IRF3 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >321 publications with IRF3 and Inflammation
 Necrosis Antibodies  >216 publications with IRF3 and Necrosis
 Liver Diseases Antibodies  >101 publications with IRF3 and Liver Diseases
 Kidney Diseases Antibodies  >38 publications with IRF3 and Kidney Diseases
 Atherosclerosis Antibodies  >19 publications with IRF3 and Atherosclerosis
 Neoplasms, Experimental Antibodies  >13 publications with IRF3 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >12 publications with IRF3 and Carcinoma, Hepatocellular
 Cell Transformation, Neoplastic Antibodies  >12 publications with IRF3 and Cell Transformation, Neoplastic
 Fibrosis Antibodies  >12 publications with IRF3 and Fibrosis
 Heart Diseases Antibodies  >11 publications with IRF3 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >277 publications with IRF3 and Blood
 Liver Antibodies  >113 publications with IRF3 and Liver
 Kidney Antibodies  >89 publications with IRF3 and Kidney
 Bone Antibodies  >88 publications with IRF3 and Bone
 Lung Antibodies  >81 publications with IRF3 and Lung
 Brain Antibodies  >70 publications with IRF3 and Brain
 Spleen Antibodies  >52 publications with IRF3 and Spleen
 Skin Antibodies  >44 publications with IRF3 and Skin
 Muscle Antibodies  >31 publications with IRF3 and Muscle
 Intestine Antibodies  >27 publications with IRF3 and Intestine
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