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anti-KLF4 antibody :: Mouse anti-Human KLF4 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200128
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

KLF4, Monoclonal Antibody

 Also Known As   

KLF4 antibody (clone AT4E6)

 Product Synonym Names    krueppel-like factor 4; EZF; GKLF
 Product Gene Name   

anti-KLF4 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG1, k
 Clone Number    AT4E6
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Clone    Anti-human kLF4 mAb, clone AT4E6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human kLF4 protein.
 Immunogen    Recombinant human kLF4 (1-170aa) purified from E Coli
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 Preparation and Storage    Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-KLF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-KLF4 antibody

   kLF4, which is also known as the gut-enriched kruppel-like factor (GkLF), is a zinc finger-containing transcription factor that belongs to the kruppel-like family of transcription factors. kLF4 plays important roles during the proliferation and differentiation of epithelial cells. Especially, it is found predominantly in gut and has been shown to be expressed during growth arrest. kLF4 is also involved in cell cycle control since expression of kLF4 inhibits DNA synthesis by blocking the G1/S phase of the cell cycle.
 Product Categories/Family for anti-KLF4 antibody    Enzymes & Proteases
 Applications Tested/Suitable for anti-KLF4 antibody   

ELISA (EIA), Western Blot (WB) (Cell lysate), Immunohistochemistry (IHC)

 Application Notes for anti-KLF4 antibody    The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.
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 Immunohistochemistry (IHC) of anti-KLF4 antibody    Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human kLF4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
anti-KLF4 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-KLF4 antibody    Cell lysates of HeLa and A549 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human kLF4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-KLF4 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for KLF4. It may not necessarily be applicable to this product.
 NCBI GI #    194248077
 NCBI GeneID    9314
 NCBI Accession #    NP_004226.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_004235.4 [Other Products]
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 UniProt Secondary Accession #    P78338; Q5T3J8; Q5T3J9; Q8N717; Q9UNP3; B2R8S4; B3KT79; L0R3I6; L0R4N5 [Other Products]
 UniProt Related Accession #    O43474 [Other Products]
 Molecular Weight    6,489 Da
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 NCBI Official Full Name    Krueppel-like factor 4
 NCBI Official Synonym Full Names    Kruppel-like factor 4 (gut)
 NCBI Official Symbol    KLF4 [Similar Products]
 NCBI Official Synonym Symbols   
EZF; GKLF
[Similar Products]
 NCBI Protein Information    Krueppel-like factor 4; endothelial Kruppel-like zinc finger protein; epithelial zinc finger protein EZF; gut-enriched krueppel-like factor
 UniProt Protein Name    Krueppel-like factor 4
 UniProt Synonym Protein Names   
Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor
 Protein Family    Krueppel-like factor
 UniProt Gene Name    KLF4 [Similar Products]
 UniProt Synonym Gene Names    EZF; GKLF [Similar Products]
 UniProt Entry Name    KLF4_HUMAN
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 UniProt Comments for KLF4    KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin

Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; somatic stem cell maintenance; negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of hemoglobin biosynthetic process; regulation of phosphoinositide 3-kinase activity; positive regulation of protein metabolic process; negative regulation of cell proliferation; post-embryonic hemopoiesis; epidermis morphogenesis; regulation of cell differentiation; response to retinoic acid; negative regulation of protein kinase B signaling cascade; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade
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Product References and Citations for anti-KLF4 antibody

   Yan FF, et al., (2008) Med Hypotheses. 70(4):845-7 Cho YG, et al., (2007) APMIS. 115(7):802-8
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 Research Articles on KLF4    1. High cytoplasmic expression of KLF4 was associated with better disease-specific survival and was an independently favorable prognostic factor in hepatocellular carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-KLF4 antibodyPathways associated with anti-KLF4 antibody
 Reference Product  PubMed Publications
 SP1 antibody  >43 publications with KLF4 and SP1
 CTNNB1 antibody  >33 publications with KLF4 and CTNNB1
 TGFB1 antibody  >15 publications with KLF4 and TGFB1
 TP53 antibody  >15 publications with KLF4 and TP53
 HDAC2 antibody  >11 publications with KLF4 and HDAC2
 RELA antibody  >4 publications with KLF4 and RELA
 KAT5 antibody  >2 publications with KLF4 and KAT5
 EP300 antibody  >2 publications with KLF4 and EP300
 CREBBP antibody  >1 publications with KLF4 and CREBBP
 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway antibodies  SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Synthesis, Secretion, And Deacylation Of Ghrelin Pathway antibodies  Synthesis, Secretion, And Deacylation Of Ghrelin Pathway Diagram
 Transcriptional Regulation Of Pluripotent Stem Cells Pathway antibodies  Transcriptional Regulation Of Pluripotent Stem Cells Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram
Diseases associated with anti-KLF4 antibodyOrgans/Tissues associated with anti-KLF4 antibody
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >82 publications with KLF4 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >76 publications with KLF4 and Nervous System Diseases
 Disease Models, Animal Antibodies  >57 publications with KLF4 and Disease Models, Animal
 Inflammation Antibodies  >53 publications with KLF4 and Inflammation
 Breast Neoplasms Antibodies  >39 publications with KLF4 and Breast Neoplasms
 Heart Diseases Antibodies  >34 publications with KLF4 and Heart Diseases
 Carcinoma, Squamous Cell Antibodies  >26 publications with KLF4 and Carcinoma, Squamous Cell
 Neoplasms, Experimental Antibodies  >21 publications with KLF4 and Neoplasms, Experimental
 Lung Diseases Antibodies  >20 publications with KLF4 and Lung Diseases
 Hyperplasia Antibodies  >18 publications with KLF4 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >204 publications with KLF4 and Embryonic Tissue
 Skin Antibodies  >127 publications with KLF4 and Skin
 Vascular Antibodies  >113 publications with KLF4 and Vascular
 Heart Antibodies  >81 publications with KLF4 and Heart
 Brain Antibodies  >72 publications with KLF4 and Brain
 Liver Antibodies  >55 publications with KLF4 and Liver
 Lung Antibodies  >49 publications with KLF4 and Lung
 Intestine Antibodies  >46 publications with KLF4 and Intestine
 Eye Antibodies  >44 publications with KLF4 and Eye
 Kidney Antibodies  >31 publications with KLF4 and Kidney
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