Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow KRT5 rightarrow Monoclonal KRT5  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • GOPC Antibody
 • UBR4 ELISA Kit
 • ZMPSTE24 Antibody
 • Aldehyde Dehydrogenase 2 (ALDH2) ELISA Kit
 • Growth Hormone Receptor (GH) Antibody
 • apolipoprotein C-III (APOC3) ELISA Kit
 • Anti-Smooth Muscle Antibody (ASMA) ELISA Kit
 • Soluble Fms like Tyrosine 1 (sFLT1) ELISA Kit
 • Dihydrolipoyl Transacetylase (DLAT) ELISA Kit
 • cysteinyl aspartate specific proteinases 3 (Caspase-3-CPP32) ELISA Kit
 • Alpha-1-Antichymotrypsin (SERPINA3) Antibody
 • ATG5 Antibody
 • TIM3 Antibody
 • MAT2A Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-KRT5 antibody :: Rabbit Keratin 5 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS302526
Unit / Price
  DISCONTINUED
You will be redirected to our replacement product
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Keratin 5 (KRT5), Monoclonal Antibody

★Popular Item★
 Also Known As   

Rabbit Anti-Human Keratin 5 Monoclonal Antibody (Clone SP27)

 Product Gene Name   

anti-KRT5 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    131760
Section Bar
 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    SP27
 Host    Rabbit
 Species Reactivity    Human
Predicted Reactivity: Bovine, Dog, Mouse, Pig, Rat
Section Bar
 Form/Format    0.1 ml rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide.
Section Bar
 Immunogen    Synthetic peptide from C-terminus of human cytokeratin 5.
 Control Tissue    Breast, Mesothelioma, Prostate, Prostate Carcinoma, Skin
 Cellular Localization    Cytoplasm
 Entrez Gene Code    3852
 Pathway    Stem Cell
Section Bar
 Preparation and Storage    Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
 Other Notes    Small volumes of anti-KRT5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-KRT5 antibody

   Twenty human keratins are divided into acidic (pI <5.7) and basic (pI >6.0) subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor identification. Point mutations in keratin 5 gene can cause various types of epidermolysis bullosa simplex. Keratin 5 is expressed in most epithelial cells, prostate basal cells and epithelial and biphasic mesotheliomas.
 Applications Tested/Suitable for anti-KRT5 antibody   

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)

 Application Notes for anti-KRT5 antibody    Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:100
IHC Positive Control: Skin, Breast, Prostate, Prostate Carcinoma, Mesothelioma

Western Blot
Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user.
Western Blot Positive Control: A431 Cell Lysate

Flow Cytometry
Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user.
Flow Cytometry Positive Control: A431 Cell Line
Section Bar
 Western Blot (WB) of anti-KRT5 antibody    Western Blot analysis of A431 cell lysate with keratin 5 antibody
anti-KRT5 antibody Western Blot (WB) (WB) image
Section Bar
 Immunohistochemistry (IHC) of anti-KRT5 antibody    Human prostate adenocarcinoma stained with anti-keratin 5 antibody
anti-KRT5 antibody Immunohistochemistry (IHC) (IHC) image
Section Bar
 Flow Cytometry (FC/FACS)|Flow cytometric analysis of rabbit anti-Keratin 5 (SP27) rabbit anti-Keratin 5 (SP27) compare to negative control of rabbit IgG (blue) of anti-KRT5 antibody    anti-KRT5 antibody Flow Cytometry (FC/FACS)|Flow cytometric analysis of rabbit anti-Keratin 5 (SP27) rabbit anti-Keratin 5 (SP27) compare to negative control of rabbit IgG (blue) (FC/FACS) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for KRT5. It may not necessarily be applicable to this product.
 NCBI GI #    119395754
 NCBI GeneID    3852
 NCBI Accession #    NP_000415.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000424.3 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q6PI71; Q6UBJ0; Q8TA91 [Other Products]
 UniProt Related Accession #    P13647 [Other Products]
 Molecular Weight    58 kDa [Similar Products]
Table BarTOPTable Bar
 NCBI Official Full Name    keratin, type II cytoskeletal 5
 NCBI Official Synonym Full Names    keratin 5, type II
 NCBI Official Symbol    KRT5 [Similar Products]
 NCBI Official Synonym Symbols   
K5; CK5; DDD; DDD1; EBS2; KRT5A
[Similar Products]
 NCBI Protein Information    keratin, type II cytoskeletal 5; 58 kda cytokeratin; CK-5; cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); type-II keratin Kb5
 UniProt Protein Name    Keratin, type II cytoskeletal 5
 UniProt Synonym Protein Names   
58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
 Protein Family    Keratin
 UniProt Gene Name    KRT5 [Similar Products]
 UniProt Synonym Gene Names    CK-5; K5 [Similar Products]
 UniProt Entry Name    K2C5_HUMAN
Section Bar
 NCBI Summary for KRT5    The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for KRT5    K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: mitochondrion; membrane; cytoplasm; keratin filament; plasma membrane; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: hemidesmosome assembly; epidermis development

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dowling-degos Disease 1; Epidermolysis Bullosa Simplex With Migratory Circinate Erythema; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex With Mottled Pigmentation; Epidermolysis Bullosa Simplex, Dowling-meara Type
Table BarTOPTable Bar
 Research Articles on KRT5    1. plectin interacts with keratins 5 and 14 in a process associated with epidermolysis bullosa simplex
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-KRT5 antibodyPathways associated with anti-KRT5 antibody
 Reference Product  PubMed Publications
 KRT14 antibody  >93 publications with KRT5 and KRT14
 ERBB2 antibody  >86 publications with KRT5 and ERBB2
 JUN antibody  >78 publications with KRT5 and JUN
 EGFR antibody  >67 publications with KRT5 and EGFR
 CALB2 antibody  >20 publications with KRT5 and CALB2
 KRT18 antibody  >5 publications with KRT5 and KRT18
 FOS antibody  >3 publications with KRT5 and FOS
 AMELX antibody  >1 publications with KRT5 and AMELX
 Products by Pathway  Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
Diseases associated with anti-KRT5 antibodyOrgans/Tissues associated with anti-KRT5 antibody
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >289 publications with KRT5 and Carcinoma
 Breast Neoplasms Antibodies  >185 publications with KRT5 and Breast Neoplasms
 Adenocarcinoma Antibodies  >158 publications with KRT5 and Adenocarcinoma
 Epidermolysis Bullosa Simplex Antibodies  >135 publications with KRT5 and Epidermolysis Bullosa Simplex
 Congenital Abnormalities Antibodies  >133 publications with KRT5 and Congenital Abnormalities
 Hyperplasia Antibodies  >83 publications with KRT5 and Hyperplasia
 Carcinoma, Basal Cell Antibodies  >78 publications with KRT5 and Carcinoma, Basal Cell
 Lung Diseases Antibodies  >68 publications with KRT5 and Lung Diseases
 Lung Neoplasms Antibodies  >64 publications with KRT5 and Lung Neoplasms
 Inflammation Antibodies  >47 publications with KRT5 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >327 publications with KRT5 and Skin
 Lung Antibodies  >86 publications with KRT5 and Lung
 Blood Antibodies  >53 publications with KRT5 and Blood
 Embryonic Tissue Antibodies  >45 publications with KRT5 and Embryonic Tissue
 Prostate Antibodies  >40 publications with KRT5 and Prostate
 Muscle Antibodies  >39 publications with KRT5 and Muscle
 Mouth Antibodies  >31 publications with KRT5 and Mouth
 Thymus Antibodies  >28 publications with KRT5 and Thymus
 Thyroid Antibodies  >27 publications with KRT5 and Thyroid
 Bladder Antibodies  >25 publications with KRT5 and Bladder
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions