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anti-Ki-67 antibody :: Rabbit Ki-67 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS302551
Immunohistochemistry (IHC)
Unit / Price
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 Product Name   

Ki-67, Monoclonal Antibody

★Popular Item★
 Also Known As   

Rabbit Anti-Human Ki-67 Monoclonal Antibody (Clone SP6)

 Product Gene Name   

anti-Ki-67 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    176741
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    SP6
 Host    Rabbit
 Species Reactivity    Human, Mouse
Predicted Reactivity: Bovine
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 Form/Format    0.1 ml rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide.
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 Immunogen    Synthetic peptide from C-terminus of human Ki-67 protein.
 Control Tissue    Tonsil
 Cellular Localization    Nucleus
 Entrez Gene Code    4288
 Pathway    Cancer Markers
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 Preparation and Storage    Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
 Other Notes    Small volumes of anti-Ki-67 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Ki-67 antibody

   Ki-67 is a nuclear protein which is expressed in the proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein.
 Applications Tested/Suitable for anti-Ki-67 antibody   

Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-Ki-67 antibody    Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:200
IHC Positive Control: Tonsil
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 Immunohistochemistry (IHC) of anti-Ki-67 antibody    Human tonsil stained with anti-Ki-67 antibody
anti-Ki-67 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for Ki-67. It may not necessarily be applicable to this product.
 NCBI GI #    225543215
 NCBI GeneID    4288
 NCBI Accession #    NP_001139438.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145966.1 [Other Products]
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 UniProt Secondary Accession #    Q5VWH2 [Other Products]
 UniProt Related Accession #    P46013 [Other Products]
 Molecular Weight    345 kDa and 395 kDa
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 NCBI Official Full Name    antigen KI-67 isoform 2
 NCBI Official Synonym Full Names    marker of proliferation Ki-67
 NCBI Official Symbol    MKI67 [Similar Products]
 NCBI Official Synonym Symbols   
KIA; MIB-; MIB-1; PPP1R105
[Similar Products]
 NCBI Protein Information    antigen KI-67; antigen identified by monoclonal antibody Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105
 UniProt Protein Name    Antigen KI-67
 UniProt Gene Name    MKI67 [Similar Products]
 UniProt Entry Name    KI67_HUMAN
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 NCBI Summary for Ki-67    This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
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 UniProt Comments for Ki-67    KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein binding; ATP binding

Biological Process: meiosis; cell proliferation
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 Research Articles on Ki-67    1. The significant positive correlations were observed between Bcl-2 protein and Ki-67 antigen in sinonasal inverted papilloma and sinonasal carcinoma groups and between Bcl-2 protein and CD34 antigen in the chronic rhinosinusitis group.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Ki-67 antibodyDiseases associated with anti-Ki-67 antibody
 Reference Product  PubMed Publications
 BIRC5 antibody  >113 publications with Ki-67 and BIRC5
 TOP2A antibody  >27 publications with Ki-67 and TOP2A
 CDK1 antibody  >17 publications with Ki-67 and CDK1
 MKI67IP antibody  >10 publications with Ki-67 and MKI67IP
 CCNA2 antibody  >9 publications with Ki-67 and CCNA2
 PRC1 antibody  >2 publications with Ki-67 and PRC1
 KIF11 antibody  >2 publications with Ki-67 and KIF11
 KIF15 antibody  >1 publications with Ki-67 and KIF15
 NCAPG antibody  >1 publications with Ki-67 and NCAPG
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >2363 publications with Ki-67 and Adenocarcinoma
 Breast Neoplasms Antibodies  >1838 publications with Ki-67 and Breast Neoplasms
 Adenoma Antibodies  >740 publications with Ki-67 and Adenoma
 Hyperplasia Antibodies  >632 publications with Ki-67 and Hyperplasia
 Colorectal Neoplasms Antibodies  >630 publications with Ki-67 and Colorectal Neoplasms
 Necrosis Antibodies  >594 publications with Ki-67 and Necrosis
 Neoplasms, Experimental Antibodies  >583 publications with Ki-67 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >581 publications with Ki-67 and Lung Neoplasms
 Skin Neoplasms Antibodies  >562 publications with Ki-67 and Skin Neoplasms
 Disease Models, Animal Antibodies  >481 publications with Ki-67 and Disease Models, Animal
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