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anti-Kl antibody :: Rat anti-Mouse Klotho Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS690455 anti-Kl antibody
Unit / Price
0.1 mg  /  $505 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Klotho, Monoclonal Antibody

★Popular Item★
 Also Known As   

Rat Anti-Mouse Klotho

 Product Synonym Names    Anti-mouse Klotho (#4F54)
 Product Gene Name   

anti-Kl antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 5; NC_000071.6 (150952607..150993817). Location: 5; 5 G3
 3D Structure    ModBase 3D Structure for O35082
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 Clonality    Monoclonal
 Isotype    IgG2
 Clone Number    (4F54)
 Host    Rat
 Species Reactivity    Mouse
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 Specificity    This antibody detects mouse Klotho in Western blotting.
 Purity/Purification    Protein A/G chromatography
 Form/Format    The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at
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 Antibody Generation    This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of Klotho. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
 Antigen    Recombinant mouse Klotho
 Reconstitution buffer    PBS (sterile)
 Reconstitution    Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
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 Preparation and Storage    Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-Kl antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Kl antibody

   The Klotho proteins, Klotho and Klotho beta (beta KL), are type I transmembrane glycoproteins with extracellular regions that contain two beta-glycosidase-like repeats. Klotho has both membrane-bound and soluble forms; the soluble form predominates and consists of only the first beta-glycosidase-like repeat. Klotho has beta-glucuronidase activity against select steroid beta-glucuronides, appears to regulate calcium and vitamin D levels in serum, and is associated with premature aging. Klotho beta, a divergent structural member of the glycosidase I superfamily, is primarily expressed in the liver and pancreas, with lower expression in adipose tissue. Klotho beta mediates the interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver, which negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes. Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes. The expression pattern of Klotho beta suggests it may play a role in lipid metabolism.
 Applications Tested/Suitable for anti-Kl antibody   

Immunohistochemistry (IHC) - Formalin, Western Blot (WB)

 Application Notes for anti-Kl antibody    Western blot: 1:500-1000
IHC (Frozen): 1:50-200
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NCBI/Uniprot data below describe general gene information for Kl. It may not necessarily be applicable to this product.
 NCBI GI #    227330584
 NCBI GeneID    16591
 NCBI Accession #    NP_038851.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013823.2 [Other Products]
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 UniProt Primary Accession #    O35082 [Other Products]
 UniProt Secondary Accession #    O70175; O70621; B2RR78 [Other Products]
 UniProt Related Accession #    O35082 [Other Products]
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 NCBI Official Full Name    klotho
 NCBI Official Synonym Full Names    klotho
 NCBI Official Symbol    Kl [Similar Products]
 NCBI Official Synonym Symbols   
alpha-kl
[Similar Products]
 NCBI Protein Information    klotho; secreted form of Klotho protein
 UniProt Protein Name    Klotho
 Protein Family    Klotho
 UniProt Gene Name    Kl [Similar Products]
 UniProt Entry Name    KLOT_MOUSE
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 UniProt Comments for Kl    Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Ref.1 Ref.5 Ref.6 Ref.9 Ref.14 Ref.15The Klotho peptide generated by cleavage of the membrane-bound isoform maybe an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. Ref.1 Ref.5 Ref.6 Ref.9 Ref.14 Ref.15

Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol. Ref.13 Ref.14

Enzyme regulation: Inhibited by D-saccharic acid 1,4-lactone and taurocholic acid. Ref.13

Subunit structure: Homodimer. Interacts with FGF23 and FGFR1. Ref.12 Ref.15

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note: Isoform 1 shedding leads to a soluble peptide. Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14Isoform 2: Secreted Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14. Klotho peptide: Secreted Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14.

Tissue specificity: Membrane-bound protein is present in distal renal tubules, inner ear, ependymal cells of brain choroid plexus, elongating spermatids and mature oocytes (at protein level). Soluble peptide is present in serum (100 pM) and cerebrospinal fluid. Expressed strongly in kidney, moderately in brain choroid plexus, and at low levels in pituitary, placenta, skeletal muscle, urinary bladder, aorta, pancreas, testis, ovary, colon, thyroid gland and adipocytes. Ref.1 Ref.2 Ref.4 Ref.7 Ref.8 Ref.11 Ref.12 Ref.14

Developmental stage: Not expressed in the embryo. Expressed in the kidney of newborns. Ref.1

Induction: Induced by 1,25-dihydroxyvitamin D3 in kidney. Down-regulated by angiotensin II and up-regulated by statins through modulation of the RhoA pathway in epithelial cells (in vitro). Isoform 1 (but not isoform 2)is up-regulated by thyroid hormone in adipocytes. Ref.7 Ref.9 Ref.10 Ref.13

Domain: Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 874.

Post-translational modification: N-glycosylated. Ref.12

Disruption phenotype: Mice display a syndrome resembling to human aging, with short lifespan, infertility, atherosclerosis, skin atrophy, osteoporosis and emphysema. They have various metabolic abnormalities, including increased insulin sensitivity and decreased insulin production. Mice overexpressing Kl have increased resistance to insulin and IGF1, a lifespan extended of more than 20%, and generate fewer offspring. Ref.5

Sequence similarities: Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Biophysicochemical propertiesKinetic parameters:KM=0.249 mM for 4-methylumbelliferylglucuronide Ref.13KM=0.251 mM for estrone 3-beta-D-glucuronideKM=0.174 mM for beta-estradiol 3-beta-D-glucuronideKM=0.251 mM for estriol 3-beta-D-glucuronideVmax=0.62 µM/h/µg enzymepH dependence:Optimum pH is 5.5.
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 Research Articles on Kl    1. In klotho mutant mice, inactivation of the JAK2/STAT3 signaling axis and M1 mAChR downregulation plays a critical role in cognitive impairment.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Kl antibodyPathways associated with anti-Kl antibody
 Reference Product  PubMed Publications
 Fgf23 antibody  >30 publications with Kl and Fgf23
 Fgf15 antibody  >1 publications with Kl and Fgf15
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
Diseases associated with anti-Kl antibodyOrgans/Tissues associated with anti-Kl antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >295 publications with Kl and Neoplasms
 Pneumonia Antibodies  >197 publications with Kl and Pneumonia
 Cardiovascular Diseases Antibodies  >121 publications with Kl and Cardiovascular Diseases
 Skin Diseases Antibodies  >114 publications with Kl and Skin Diseases
 Pulmonary Fibrosis Antibodies  >113 publications with Kl and Pulmonary Fibrosis
 Inflammation Antibodies  >79 publications with Kl and Inflammation
 Heart Diseases Antibodies  >71 publications with Kl and Heart Diseases
 Kidney Diseases Antibodies  >53 publications with Kl and Kidney Diseases
 Breast Neoplasms Antibodies  >45 publications with Kl and Breast Neoplasms
 Necrosis Antibodies  >41 publications with Kl and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >222 publications with Kl and Brain
 Liver Antibodies  >121 publications with Kl and Liver
 Kidney Antibodies  >119 publications with Kl and Kidney
 Testis Antibodies  >30 publications with Kl and Testis
 Embryonic Tissue Antibodies  >24 publications with Kl and Embryonic Tissue
 Thymus Antibodies  >18 publications with Kl and Thymus
 Pancreas Antibodies  >12 publications with Kl and Pancreas
 Inner Ear Antibodies  >4 publications with Kl and Inner Ear
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