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anti-LGR5 antibody :: Mouse LGR5/GPR49 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9200598
Western Blot (WB)
Unit / Price
0.1 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

LGR5/GPR49, Monoclonal Antibody

★Popular Item★
 Also Known As   

LGR5/GPR49 Antibody (Ascites)

 Product Synonym Names    Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; LGR5; GPR49; GPR67
 Product Gene Name   

anti-LGR5 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    443-473
 OMIM    606667
 3D Structure    ModBase 3D Structure for O75473
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    349CT16.1.3.1
 Host    Mouse
 Species Reactivity    Human (Predicted Reactivity: Bovine)
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 Specificity    This LGR5/GPR49 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from human LGR5/GPR49.
 Form/Format    Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
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 Function    Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
 Cellular Location    Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).
 Tissue Location    Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base.Overexpressed in cancers of the ovary, colon and liver.
    
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-LGR5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-LGR5 antibody

   Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
 Product Categories/Family for anti-LGR5 antibody    Cancer; Signal Transduction; GPCR Antibodies
 Applications Tested/Suitable for anti-LGR5 antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-LGR5 antibody    WB~~1:1000~8000
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 Western Blot (WB) of anti-LGR5 antibody    LGR5/GPR49 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).
anti-LGR5 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for LGR5. It may not necessarily be applicable to this product.
 NCBI GI #    469608414
 NCBI GeneID    8549
 NCBI Accession #    NP_001264155.1 [Other Products]
 NCBI Related Accession #    NP_003658.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001277226.1 [Other Products]
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 UniProt Primary Accession #    O75473 [Other Products]
 UniProt Secondary Accession #    Q4VAM0; Q4VAM2; Q9UP75; D8MCT0 [Other Products]
 UniProt Related Accession #    O75473 [Other Products]
 Molecular Weight    99998
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 NCBI Official Full Name    leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
 NCBI Official Synonym Full Names    leucine-rich repeat containing G protein-coupled receptor 5
 NCBI Official Symbol    LGR5 [Similar Products]
 NCBI Official Synonym Symbols   
FEX; HG38; GPR49; GPR67; GRP49
[Similar Products]
 NCBI Protein Information    leucine-rich repeat-containing G-protein coupled receptor 5
 UniProt Protein Name    Leucine-rich repeat-containing G-protein coupled receptor 5
 UniProt Synonym Protein Names   
G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
 Protein Family    Leucine-rich repeat-containing G-protein coupled receptor
 UniProt Gene Name    LGR5 [Similar Products]
 UniProt Synonym Gene Names    GPR49; GPR67 [Similar Products]
 UniProt Entry Name    LGR5_HUMAN
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 NCBI Summary for LGR5    The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
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 UniProt Comments for LGR5    LGR5: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A marker of cycling, long-lived and multipotent adult stem cell populations. Target gene of Wnt signaling. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Intestinal Lgr5+ stem cells give rise throughout life to proliferation of cells in crypts, which migrate along the crypt-villus axis, differentiating into five main cell types. Wnt signaling plays a crucial role in the proliferation and differentiation of these crypt epithelial cells. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: integral to plasma membrane; plasma membrane; trans-Golgi network membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; inner ear development
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Product References and Citations for anti-LGR5 antibody

   Fontaine-Bisson, B., et al. Diabetologia 53(10):2155-2162(2010)
Voight, B.F., et al. Nat. Genet. 42(7):579-589(2010)
Uchida, H., et al. Cancer Sci. 101(7):1731-1737(2010)
Fan, X.S., et al. Int J Colorectal Dis 25(5):583-590(2010)
Acevedo, A.C., et al. J. Dent. Res. 89(2):128-132(2010)
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 Research Articles on LGR5    1. the findings of this study suggest that LGR5 plays a vital role in CRC pathogenesis and has the potential to serve as a diagnostic marker and a therapeutic target for CRC patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-LGR5 antibodyDiseases associated with anti-LGR5 antibody
 Reference Product  PubMed Publications
 RSPO1 antibody  >24 publications with LGR5 and RSPO1
 RNF43 antibody  >9 publications with LGR5 and RNF43
 ZNRF3 antibody  >9 publications with LGR5 and ZNRF3
 LRP6 antibody  >7 publications with LGR5 and LRP6
 RSPO3 antibody  >6 publications with LGR5 and RSPO3
 RSPO2 antibody  >4 publications with LGR5 and RSPO2
 RSPO4 antibody  >3 publications with LGR5 and RSPO4
 Disease Name  Pubmed Publications
 Colonic Neoplasms Antibodies  >58 publications with LGR5 and Colonic Neoplasms
 Inflammation Antibodies  >30 publications with LGR5 and Inflammation
 Neoplasm Metastasis Antibodies  >28 publications with LGR5 and Neoplasm Metastasis
 Liver Diseases Antibodies  >22 publications with LGR5 and Liver Diseases
 Disease Models, Animal Antibodies  >20 publications with LGR5 and Disease Models, Animal
 Liver Neoplasms Antibodies  >18 publications with LGR5 and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >15 publications with LGR5 and Neoplasms, Experimental
 Precancerous Conditions Antibodies  >11 publications with LGR5 and Precancerous Conditions
 Necrosis Antibodies  >10 publications with LGR5 and Necrosis
 Hyperplasia Antibodies  >9 publications with LGR5 and Hyperplasia
Organs/Tissues associated with anti-LGR5 antibody
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >239 publications with LGR5 and Intestine
 Liver Antibodies  >55 publications with LGR5 and Liver
 Stomach Antibodies  >53 publications with LGR5 and Stomach
 Skin Antibodies  >32 publications with LGR5 and Skin
 Embryonic Tissue Antibodies  >28 publications with LGR5 and Embryonic Tissue
 Bone Antibodies  >24 publications with LGR5 and Bone
 Brain Antibodies  >15 publications with LGR5 and Brain
 Lymph Node Antibodies  >13 publications with LGR5 and Lymph Node
 Ear Antibodies  >12 publications with LGR5 and Ear
 Muscle Antibodies  >11 publications with LGR5 and Muscle
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