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anti-Ly6G antibody :: Rat Ly6G Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS832202
Flow Cytometry (FC/FACS)
Unit / Price
0.05 mg  /  $180 +1 FREE 8GB USB
0.1 mg  /  $210 +1 FREE 8GB USB
 
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 Product Name   

Ly6G, Monoclonal Antibody

 Also Known As   

Ly6G antibody (FITC)

 Product Synonym Names    Monoclonal Ly6G; Anti-Ly6G; Ly6G; LyG-6; LyG 6; Gr1
 Product Gene Name   

anti-Ly6G antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG2b kappa
 Clone Number    RB6-8C5
 Host    Rat
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 Specificity    Mouse
 Form/Format    Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
 Concentration    500 ug/ml (lot specific)
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 Biological Significance    The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations. Blocking studies with Ly-6C or RB6-8C5 show no effect against staining with the other clone thereby suggesting that RB6-8C5 does not recognize Ly-6C in resting bone marrow or splenocytes.
 Tag/Conjugate    FITC
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 Preparation and Storage    Store at 2-8 degree C. Do not freeze. Protect from light.
 Other Notes    Small volumes of anti-Ly6G antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-Ly6G antibody

   Rat monoclonal Ly6G antibody (FITC)
 Product Categories/Family for anti-Ly6G antibody    Differentiation & Development; Conjugated Monoclonal Antibodies
 Applications Tested/Suitable for anti-Ly6G antibody   

Flow Cytometry (FC/FACS)

 Application Notes for anti-Ly6G antibody    FC: <= 0.25 ug/test

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 ug of purified anti-mouse CD16/32 (cat. 14-0161). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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 Flow Cytometry (FC/FACS) of anti-Ly6G antibody    Staining of mouse bone marrow cells with CD45R antibody (PE) and 0.25 ug of Rat IgG2b K Isotype Control (FITC) (left) or 0.03 ug of Ly-6G antibody (FITC) (right). Total viable cells were used for analysis.
anti-Ly6G antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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NCBI/Uniprot data below describe general gene information for Ly6G. It may not necessarily be applicable to this product.
 NCBI GI #    547876
 NCBI Accession #    P35461.1 [Other Products]
 Molecular Weight    11,798 Da
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 NCBI Official Full Name    Lymphocyte antigen 6G
 UniProt Protein Name    Lymphocyte antigen 6G
 UniProt Synonym Protein Names   
Ly-6G.1
 Protein Family    Lymphocyte antigen
 UniProt Gene Name    Ly6g [Similar Products]
 UniProt Synonym Gene Names    Ly-6G [Similar Products]
 UniProt Entry Name    LY6G_MOUSE
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 UniProt Comments for Ly6G    Ly6g:

Cellular Component: membrane; plasma membrane; external side of plasma membrane
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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