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anti-S100A8 antibody :: Mouse MACROPHAGES/MONOCYTES Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS211113
Unit / Price
0.1 mg  /  $390 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MACROPHAGES/MONOCYTES (S100A8), Monoclonal Antibody

 Also Known As   

MOUSE ANTI HUMAN MACROPHAGES:FITC

 Product Gene Name   

anti-S100A8 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    123885
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    MAC387
 Host    Mouse
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 Form/Format    FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
 Concentration    IgG concentration 0.1 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
 Buffer Solution    Phosphate buffered saline
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-S100A8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-S100A8 antibody

   Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen recognized by clone MCA387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.
 Applications Tested/Suitable for anti-S100A8 antibody   

Flow cytometry (FC/FACS)*

 Application Notes for anti-S100A8 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
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 Testing Data of anti-S100A8 antibody    Staining of human peripheral blood granulocytes with Mouse anti Human macrophages:FITC. Permeabilised with Leucoperm
anti-S100A8 antibody Testing Data image
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 Testing Data of anti-S100A8 antibody    Staining of human peripheral blood granulocytes with Mouse anti Human macrophages (MBS219286). Permeabilised with Leucoperm
anti-S100A8 antibody Testing Data image
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 Testing Data of anti-S100A8 antibody    Macrophages stained with Mouse anti Human Macrophages/ Monocytes (MBS216638) using enhanced DAB in experimental allergic marmoset brain. Mag. X 400
anti-S100A8 antibody Testing Data image
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 Testing Data of anti-S100A8 antibody    Staining of human peripheral blood granulocytes with Mouse anti Human Macrophages:RPE (MBS224589). Permeabilised with Leucoperm
anti-S100A8 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for S100A8. It may not necessarily be applicable to this product.
 NCBI GI #    21614544
 NCBI GeneID    6279
 NCBI Accession #    NP_002955.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002964.4 [Other Products]
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 UniProt Secondary Accession #    Q5SY70; Q9UC84; Q9UC92; Q9UCJ0; Q9UCM6; A8K5L3; D3DV37 [Other Products]
 UniProt Related Accession #    P05109 [Other Products]
 Molecular Weight    10,835 Da [Similar Products]
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 NCBI Official Full Name    protein S100-A8
 NCBI Official Synonym Full Names    S100 calcium binding protein A8
 NCBI Official Symbol    S100A8 [Similar Products]
 NCBI Official Synonym Symbols   
P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
[Similar Products]
 NCBI Protein Information    protein S100-A8; MRP-8; S100 calcium-binding protein A8 (calgranulin A); calgranulin-A; calprotectin L1L subunit; cystic fibrosis antigen; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; urinary stone protein band A
 UniProt Protein Name    Protein S100-A8
 UniProt Synonym Protein Names   
Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; S100 calcium-binding protein A8; Urinary stone protein band AProtein S100-A8, N-terminally processed
 Protein Family    Protein
 UniProt Gene Name    S100A8 [Similar Products]
 UniProt Synonym Gene Names    CAGA; CFAG; MRP8; CFAG; MRP-8; p8 [Similar Products]
 UniProt Entry Name    S10A8_HUMAN
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 NCBI Summary for S100A8    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for S100A8    S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cytoskeleton; plasma membrane; extracellular region; nucleus; cytosol

Molecular Function: arachidonic acid binding; RAGE receptor binding; protein binding; zinc ion binding; microtubule binding; calcium ion binding

Biological Process: caspase activation; neutrophil chemotaxis; chemokine production; chronic inflammatory response; wound healing; cytokine production; positive regulation of peptide secretion; response to lipopolysaccharide; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; response to zinc ion; defense response to bacterium; autophagy; innate immune response; inflammatory response; defense response to fungus; acute inflammatory response; positive regulation of inflammatory response; regulation of cytoskeleton organization and biogenesis
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 Research Articles on S100A8    1. these data reveal the capacity of these three S100 proteins to induce MUC5AC production in airway epithelial cells, suggesting that they all serve as key mediators linking neutrophil-dominant airway inflammation to mucin hyperproduction.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-S100A8 antibodyDiseases associated with anti-S100A8 antibody
 Reference Product  PubMed Publications
 S100A9 antibody  >741 publications with S100A8 and S100A9
 S100A12 antibody  >101 publications with S100A8 and S100A12
 TLR4 antibody  >56 publications with S100A8 and TLR4
 MYD88 antibody  >11 publications with S100A8 and MYD88
 CSF3 antibody  >2 publications with S100A8 and CSF3
 MNDA antibody  >2 publications with S100A8 and MNDA
 TIRAP antibody  >2 publications with S100A8 and TIRAP
 LY96 antibody  >1 publications with S100A8 and LY96
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >410 publications with S100A8 and Inflammation
 Fibrosis Antibodies  >103 publications with S100A8 and Fibrosis
 Disease Models, Animal Antibodies  >95 publications with S100A8 and Disease Models, Animal
 Necrosis Antibodies  >80 publications with S100A8 and Necrosis
 Cardiovascular Diseases Antibodies  >75 publications with S100A8 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >60 publications with S100A8 and Nervous System Diseases
 Heart Diseases Antibodies  >30 publications with S100A8 and Heart Diseases
 Kidney Diseases Antibodies  >25 publications with S100A8 and Kidney Diseases
 Lung Neoplasms Antibodies  >25 publications with S100A8 and Lung Neoplasms
 Breast Neoplasms Antibodies  >24 publications with S100A8 and Breast Neoplasms
Organs/Tissues associated with anti-S100A8 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >492 publications with S100A8 and Blood
 Skin Antibodies  >131 publications with S100A8 and Skin
 Lung Antibodies  >112 publications with S100A8 and Lung
 Vascular Antibodies  >88 publications with S100A8 and Vascular
 Bone Antibodies  >64 publications with S100A8 and Bone
 Brain Antibodies  >55 publications with S100A8 and Brain
 Bone Marrow Antibodies  >52 publications with S100A8 and Bone Marrow
 Kidney Antibodies  >43 publications with S100A8 and Kidney
 Liver Antibodies  >42 publications with S100A8 and Liver
 Muscle Antibodies  >39 publications with S100A8 and Muscle
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