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anti-MET antibody :: Mouse anti-Human, mouse MET/HGFR Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9200046
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MET/HGFR, Monoclonal Antibody

★Popular Item★
 Also Known As   

MET/HGFR Antibody

 Product Synonym Names    Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET
 Product Gene Name   

anti-MET antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    114550
 3D Structure    ModBase 3D Structure for P08581
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 Clonality    Monoclonal
 Isotype    Mouse IgG1
 Clone Number    4AT259
 Host    Mouse
 Species Reactivity    Human, mouse
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 Specificity    This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met.
 Purity/Purification    Purified Mouse Monoclonal Antibody (Mab)
 Form/Format    fomat Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
 Concentration    Vial Concentration: 10 (lot specific)
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 Antigen Source    HUMAN
 Function    Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity)
 Cellular Location    Membrane; Single-pass type I membrane protein
  Tissue Location    Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. Expressed in metaphyseal bone (at protein level) (PubMed:26637977)
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-MET antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MET antibody

   The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
 Product Categories/Family for anti-MET antibody    Cancer; Signal Transduction
 Applications Tested/Suitable for anti-MET antibody   

Immunofluorescence (IF), ELISA (EIA), Western Blot (WB)

 Application Notes for anti-MET antibody    IF~~1:200
WB~~1:100~500
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 Immunofluorescence (IF) of anti-MET antibody    Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9200046 MET/HGFR primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).
anti-MET antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-MET antibody    Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent MyBioSource ’s different anti-Met monoclonal antibodies that are. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab.
anti-MET antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for MET. It may not necessarily be applicable to this product.
 NCBI GI #    42741655
 NCBI GeneID    4233
 NCBI Accession #    NP_000236.2 [Other Products]
 NCBI Related Accession #    NP_001120972.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000245.2 [Other Products]
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 UniProt Primary Accession #    P08581 [Other Products]
 UniProt Secondary Accession #    O60366; Q12875; Q9UDX7; Q9UPL8; A1L467; B5A932; E7EQ94 [Other Products]
 UniProt Related Accession #    P08581 [Other Products]
 Molecular Weight    155541
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 NCBI Official Full Name    hepatocyte growth factor receptor isoform b
 NCBI Official Synonym Full Names    MET proto-oncogene, receptor tyrosine kinase
 NCBI Official Symbol    MET [Similar Products]
 NCBI Official Synonym Symbols   
HGFR; AUTS9; RCCP2; c-Met; DFNB97
[Similar Products]
 NCBI Protein Information    hepatocyte growth factor receptor
 UniProt Protein Name    Hepatocyte growth factor receptor
 UniProt Synonym Protein Names   
HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
 Protein Family    Hepatocyte growth factor receptor
 UniProt Gene Name    MET [Similar Products]
 UniProt Synonym Gene Names    HGF receptor; SF receptor [Similar Products]
 UniProt Entry Name    MET_HUMAN
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 NCBI Summary for MET    The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MET    Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.

Protein type: Oncoprotein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; Met family

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: cell surface; integral to plasma membrane; extracellular region; basal plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; protein phosphatase binding; hepatocyte growth factor receptor activity; ATP binding

Biological Process: axon guidance; skeletal muscle development; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; myoblast proliferation; myotube differentiation; muscle cell migration; hepatocyte growth factor receptor signaling pathway; glucose homeostasis; liver development; signal transduction; cell proliferation; cell surface receptor linked signal transduction; positive chemotaxis; branching morphogenesis of a tube; adult behavior; positive regulation of transcription from RNA polymerase II promoter; endothelial cell morphogenesis; brain development; placenta development

Disease: Hepatocellular Carcinoma; Renal Cell Carcinoma, Papillary, 1
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Product References and Citations for anti-MET antibody

   References for protein:
1.MET receptor sequence variants R970C and T992I lack transforming capacity. Tyner JW, et al. Cancer Res, 2010 Aug 1. PMID 20670955.
2.Further evidence for the role of MET in autism susceptibility. Thanseem I, et al. Neurosci Res, 2010 Oct. PMID 20615438.
3.Increased HGF and c-Met in muscle tissues of polymyositis and dermatomyositis patients: beneficial roles of HGF in muscle regeneration. Sugiura T, et al. Clin Immunol, 2010 Sep. PMID 20580899.
4.Correlation between hepatocyte growth factor receptor and vascular endothelial growth factor-A in breast carcinoma. Gisterek I, et al. Folia Histochem Cytobiol, 2010 Jan 1. PMID 20529820.
5.MET overexpressing chordomas frequently exhibit polysomy of chromosome 7 but no MET activation through sarcoma-specific gene fusions. Grabellus F, et al. Tumour Biol, 2010 Jun. PMID 20512480.
References for HepG2 cell line:
1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960].
2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851].
3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761-1775. [PubMed:7506266].
4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1-3): 329-340. [PubMed:15138059].
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 Research Articles on MET    1. Report poor prognosis/survival in luminal breast cancer patients with circulating tumor cells co-expressing MET and CD47.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MET antibodyPathways associated with anti-MET antibody
 Reference Product  PubMed Publications
 HGF antibody  >2791 publications with MET and HGF
 CTNNB1 antibody  >230 publications with MET and CTNNB1
 UBC antibody  >166 publications with MET and UBC
 GRB2 antibody  >119 publications with MET and GRB2
 GAB1 antibody  >103 publications with MET and GAB1
 CBL antibody  >65 publications with MET and CBL
 CDH1 antibody  >38 publications with MET and CDH1
 PTPN11 antibody  >21 publications with MET and PTPN11
 PLXNB1 antibody  >20 publications with MET and PLXNB1
 PTPN1 antibody  >8 publications with MET and PTPN1
 Products by Pathway  Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
 Bisphenol Degradation Pathway antibodies  Bisphenol Degradation Pathway Diagram
Diseases associated with anti-MET antibodyOrgans/Tissues associated with anti-MET antibody
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >4969 publications with MET and Carcinoma
 Heart Diseases Antibodies  >4678 publications with MET and Heart Diseases
 Inflammation Antibodies  >2518 publications with MET and Inflammation
 Adenocarcinoma Antibodies  >2290 publications with MET and Adenocarcinoma
 Liver Diseases Antibodies  >2183 publications with MET and Liver Diseases
 Kidney Diseases Antibodies  >1904 publications with MET and Kidney Diseases
 Schizophrenia Antibodies  >1851 publications with MET and Schizophrenia
 Breast Neoplasms Antibodies  >1839 publications with MET and Breast Neoplasms
 Lung Neoplasms Antibodies  >1624 publications with MET and Lung Neoplasms
 Neoplasm Metastasis Antibodies  >1496 publications with MET and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9030 publications with MET and Brain
 Heart Antibodies  >6142 publications with MET and Heart
 Bone Antibodies  >4839 publications with MET and Bone
 Lung Antibodies  >4545 publications with MET and Lung
 Vascular Antibodies  >4329 publications with MET and Vascular
 Liver Antibodies  >4210 publications with MET and Liver
 Muscle Antibodies  >3952 publications with MET and Muscle
 Kidney Antibodies  >3016 publications with MET and Kidney
 Skin Antibodies  >2404 publications with MET and Skin
 Eye Antibodies  >1896 publications with MET and Eye
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