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anti-MIF antibody :: Mouse anti-Human MIF Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200026
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MIF, Monoclonal Antibody

★Popular Item★
 Also Known As   

MIF antibody

 Product Synonym Names    Macrophage migration inhibitory factor; GLIF; MMIF; MIF; EC 5.3.2.1; Phenylpyruvate tautomerase; Glycosylation-inhibiting factor; GIF; Macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor)
 Product Gene Name   

anti-MIF antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    M25639 mRNA
 3D Structure    ModBase 3D Structure for P14174
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 Clonality    Monoclonal
 Isotype    IgG1, k
 Clone Number    4E4
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Immunogen    Recombinant human MIF (1-114 aa) purified from E. coli
 Clone Note    Anti-human MIF mAb, clone 4E4, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MIF protein.
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 Preparation and Storage    Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-MIF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MIF antibody

   The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
 Product Categories/Family for anti-MIF antibody    Immunology
 Applications Tested/Suitable for anti-MIF antibody   

ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

 Application Notes for anti-MIF antibody    WB: 1:500 ~ 2,000.
Recommended starting dilution is 1:1,000.
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 Western Blot (WB) of anti-MIF antibody    The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: THP-1 cell lysate Lane 3.: HeLa cell lysate Lane 4.: U937 cell lysate The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-MIF antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-MIF antibody    ICC/IF analysis of MIF in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MIF antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of MIF in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MIF antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-MIF antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for MIF. It may not necessarily be applicable to this product.
 NCBI GI #    4505185
 NCBI GeneID    4282
 NCBI Accession #    NP_002406 [Other Products]
 NCBI GenBank Nucleotide #    NM_002415.1 [Other Products]
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 UniProt Primary Accession #    P14174 [Other Products]
 UniProt Secondary Accession #    Q2V4Y5; Q6FHV0; A5Z1R8; B2R4S3 [Other Products]
 UniProt Related Accession #    P14174 [Other Products]
 Molecular Weight    12,476 Da
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 NCBI Official Full Name    macrophage migration inhibitory factor
 NCBI Official Synonym Full Names    macrophage migration inhibitory factor
 NCBI Official Symbol    MIF [Similar Products]
 NCBI Official Synonym Symbols   
GIF; GLIF; MMIF
[Similar Products]
 NCBI Protein Information    macrophage migration inhibitory factor
 UniProt Protein Name    Macrophage migration inhibitory factor
 UniProt Synonym Protein Names   
Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase
 Protein Family    MIF-like protein
 UniProt Gene Name    MIF [Similar Products]
 UniProt Synonym Gene Names    GLIF; MMIF; MIF; GIF [Similar Products]
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 NCBI Summary for MIF    This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MIF    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
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Product References and Citations for anti-MIF antibody

   Weiser WY, et al., (1989) Proc Natl Acad Sci. 86:7522-7526.; Bernhagen J, et al., (1994) Biochemistry. 33:14144-14155; Richard B, et al., (1996) FASEB J. 10:1607-1613
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 Research Articles on MIF    1. The assay monitors the increase in absorbance at 320 nm resulting from keto-to-enol tautomerization of 4-hydroxyphenylpyruvate, a reaction catalyzed by MIF. We ran a full-diversity screen evaluating the inhibitory activity of 1.6 million compounds.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MIF antibodyPathways associated with anti-MIF antibody
 Reference Product  PubMed Publications
 CD74 antibody  >216 publications with MIF and CD74
 TAT antibody  >4 publications with MIF and TAT
 PARK7 antibody  >2 publications with MIF and PARK7
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Neutrophil Degranulation Pathway antibodies  Neutrophil Degranulation Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
Diseases associated with anti-MIF antibodyOrgans/Tissues associated with anti-MIF antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >909 publications with MIF and Inflammation
 Necrosis Antibodies  >384 publications with MIF and Necrosis
 Disease Models, Animal Antibodies  >303 publications with MIF and Disease Models, Animal
 Carcinoma Antibodies  >193 publications with MIF and Carcinoma
 Sepsis Antibodies  >162 publications with MIF and Sepsis
 Heart Diseases Antibodies  >144 publications with MIF and Heart Diseases
 Arthritis, Rheumatoid Antibodies  >131 publications with MIF and Arthritis, Rheumatoid
 Liver Diseases Antibodies  >109 publications with MIF and Liver Diseases
 Kidney Diseases Antibodies  >103 publications with MIF and Kidney Diseases
 Pneumonia Antibodies  >93 publications with MIF and Pneumonia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1750 publications with MIF and Blood
 Brain Antibodies  >468 publications with MIF and Brain
 Vascular Antibodies  >328 publications with MIF and Vascular
 Lung Antibodies  >263 publications with MIF and Lung
 Muscle Antibodies  >243 publications with MIF and Muscle
 Skin Antibodies  >234 publications with MIF and Skin
 Liver Antibodies  >217 publications with MIF and Liver
 Heart Antibodies  >205 publications with MIF and Heart
 Spleen Antibodies  >175 publications with MIF and Spleen
 Kidney Antibodies  >156 publications with MIF and Kidney
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