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anti-MMP9 antibody :: Mouse anti-Human MMP-9 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS670154
Testing Data
Unit / Price
0.1 mg  /  $265 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MMP-9 (MMP9), Monoclonal Antibody

 Also Known As   

Mouse Anti-Human MMP-9-UNLB

 Product Synonym Names    Mouse Anti-Human MMP-9 - Purified, Unlabeled Antibody; Mouse Anti-Human MMP-9
 Product Gene Name   

anti-MMP9 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 20; NC_000020.10 (44637547..44645200). Location: 20q11.2-q13.1
 OMIM    120361
 3D Structure    ModBase 3D Structure for P14780
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 Clonality    Monoclonal
 Isotype    Mouse IgG2a
 Clone Number    SB15c
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Human MMP-9; Does not cross react to human MMP-1, MMP-2 or MMP-3
Matrix metalloproteinases (MMPs) are a family of at least 20 structurally related, zinc-containing enzymes that have the ability to breakdown connective tissue. MMP-9 is also known as gelatinase B and 92kDa gelatinase. MMP-9, along with MMP-2, belongs to the gelatinase group of MMP's which have three repeats of a type II fibronectin domain inserted in the catalytic domain. MMP-9 has shown direct and indirect substrate specificity to collagens IV, V, X and XIV, gelatin, elastin, proteoglycans, plasminogen, aggrecan, galectin-3, entactin, fibrillin, fibrin, IL-1 beta, amyloid beta peptide and myelin basic protein. 1-15
 Form/Format    Purified (UNLB) Antibody
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 Immunogen    Recombinant MMP-9
 Characterization    To insure lot-to-lot consistency, each batch of product is tested by ELISA to conform with the characteristics of a standard reference reagent.
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 Preparation and Storage    The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
 Other Notes    Small volumes of anti-MMP9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-MMP9 antibody   

ELISA; Immunohistochemistry_PES

 Application Notes for anti-MMP9 antibody    Immunohistochemistry Suggested Dilution: <= 2 ug/mL
ELISA Suggested Dilution: 1:3,000-1:8,000
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 Testing Data of anti-MMP9 antibody    anti-MMP9 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for MMP9. It may not necessarily be applicable to this product.
 NCBI GI #    74272287
 NCBI GeneID    4318
 NCBI Accession #    NP_004985.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004994.2 [Other Products]
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 UniProt Primary Accession #    P14780 [Other Products]
 UniProt Secondary Accession #    Q3LR70; Q8N725; Q9H4Z1; Q9UCJ9; Q9UCL1; Q9UDK2; B2R7V9 [Other Products]
 UniProt Related Accession #    P14780; Q9Y354 [Other Products]
 Molecular Weight    78,458 Da [Similar Products]
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 NCBI Official Full Name    matrix metalloproteinase-9 preproprotein
 NCBI Official Synonym Full Names    matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
 NCBI Official Symbol    MMP9 [Similar Products]
 NCBI Official Synonym Symbols   
GELB; CLG4B; MMP-9; MANDP2
[Similar Products]
 NCBI Protein Information    matrix metalloproteinase-9; 92 kDa gelatinase; OTTHUMP00000031674; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
 UniProt Protein Name    Matrix metalloproteinase-9
 UniProt Synonym Protein Names   
92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B
 Protein Family    Matrix metalloproteinase
 UniProt Gene Name    MMP9 [Similar Products]
 UniProt Synonym Gene Names    CLG4B [Similar Products]
 UniProt Entry Name    MMP9_HUMAN
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 NCBI Summary for MMP9    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq]
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 UniProt Comments for MMP9    Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Ref.16

Catalytic activity: Cleavage of gelatin types I and V and collagen types IV and V. Ref.16

Cofactor: Binds 2 zinc ions per subunit.Binds 3 calcium ions per subunit.

Enzyme regulation: Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Ref.19 Ref.21

Subunit structure: Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Ref.18 Ref.21 Ref.27

Subcellular location: Secreted › extracellular space › extracellular matrix

Probable.

Tissue specificity: Produced by normal alveolar macrophages and granulocytes.

Induction: Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Ref.11 Ref.12 Ref.19 Ref.21 Ref.24

Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational modification: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.N- and O-glycosylated. Ref.9

Involvement in disease: Defects in MMP9 may be a cause of susceptibility to intervertebral disc disease (IDD) [

MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. Ref.22Defects in MMP9 are the cause of metaphyseal anadysplasia type 2 (MANDP2) [

MIM:613073]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.

Miscellaneous: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.

Sequence similarities: Belongs to the peptidase M10A family.Contains 3 fibronectin type-II domains.Contains 4 hemopexin-like domains.
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Product References and Citations for anti-MMP9 antibody

   1. Steffensen, B., U.M. Wallon, and C.M. Overall. 1995. J. Biol. Chem. 270:11555.
2. Allan, J.A., A.J. Docherty, P.J. Barker, N.S. Huskisson, J.J. Reynolds, and G. Murphy. 1995. Biochem. J. 309:229.
3. Murphy, G., M.I. Cockett, R.V. Ward, and A.J. Docherty. 1991. J. Biol. Chem. 277:277.
4. Murphy, G., J.J. Reynolds, U. Bretz, and M. Baggiolini. 1982. Biochem. J. 203:209.
5. Welgus, H.G., C.J. Fliszar, J.L. Seltzer, T.M. Schmid, and J.J. Jeffery. 1990. J. Biol. Chem. 265:13521.
6. Sires, U.I., B. Dublet, E. Aubert-Foucher, M. van der Rest, and H.G. Welgus. 1995. J. Bio. Chem. 270:1062.
7. Patterson, B.C., and Q.A. Sang. 1997. J. Biol. Chem. 272:28823.
8. Fosang, A.J., P.J. Neame, K. Last, T.E. Hardingham, G. Murphy, and J.A. Hamilton. 1992. J. Biol. Chem. 267:19470.
9. Ochieng, J., R. Fridman, P. Nangia-Makker, D.E. Kleiner, L.A. Liotta, W.G. Stetler-Stevenson, and A. Raz. 1994. Biochemistry 33:14109.
10. Sires, U.I., G.L. Griffin, T.J. Brekelmann, R.P. Mecham, G. Murphy, A.E. Chung, H.G. Welgus, and R.M. Senior. 1993. J. Biol. Chem. 268:2069.
11. Ashworth, J.L., G. Murphy, M.J. Rock, M.J. Sherratt, S.D. Shapiro, C.A. Shuttleworth, and C.M. Kielty. 1999. Biochem. J. 340:171.
12. Lelongt, B., S. Bengatta, M. Delauche, L.R. Lund, Z. Werb, and P.M. Ronco. 2001. J. Exp Med. 193:793.
13. Ito, A., A. Mukaiyama, Y. Itoh, H. Nagase, I.B. Thogersen, J.J. Enghild, Y. Sasaguri, and Y. Mori. 1996. J. Biol. Chem. 271:14657.
14. Chandler, S., R. Coates, A. Gearing, J. Lury, G. Wells, and E. Bone. 1995. Neurosci. Lett. 201:223.
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 Research Articles on MMP9    1. MMP-9 mRNA expression in peripheral blood mononuclear cells from NPC patients is increased and associated with the clinical characteristics and overall survival.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MMP9 antibodyPathways associated with anti-MMP9 antibody
 Reference Product  PubMed Publications
 MMP7 antibody  >49 publications with MMP9 and MMP7
 TIMP3 antibody  >38 publications with MMP9 and TIMP3
 MMP10 antibody  >23 publications with MMP9 and MMP10
 IL8 antibody  >19 publications with MMP9 and IL8
 CD44 antibody  >15 publications with MMP9 and CD44
 COL1A1 antibody  >12 publications with MMP9 and COL1A1
 CXCL1 antibody  >11 publications with MMP9 and CXCL1
 THBS1 antibody  >11 publications with MMP9 and THBS1
 RECK antibody  >11 publications with MMP9 and RECK
 LCN2 antibody  >8 publications with MMP9 and LCN2
 Products by Pathway  Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 LPA Receptor Mediated Events Pathway antibodies  LPA Receptor Mediated Events Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram
Diseases associated with anti-MMP9 antibodyOrgans/Tissues associated with anti-MMP9 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >381 publications with MMP9 and Neoplasms
 Carcinoma Antibodies  >189 publications with MMP9 and Carcinoma
 Cardiovascular Diseases Antibodies  >185 publications with MMP9 and Cardiovascular Diseases
 Inflammation Antibodies  >155 publications with MMP9 and Inflammation
 Neoplasm Invasiveness Antibodies  >127 publications with MMP9 and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >115 publications with MMP9 and Neoplasm Metastasis
 Lung Diseases Antibodies  >93 publications with MMP9 and Lung Diseases
 Fibrosis Antibodies  >75 publications with MMP9 and Fibrosis
 Adenocarcinoma Antibodies  >63 publications with MMP9 and Adenocarcinoma
 Breast Neoplasms Antibodies  >60 publications with MMP9 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >319 publications with MMP9 and Blood
 Lung Antibodies  >135 publications with MMP9 and Lung
 Bone Antibodies  >133 publications with MMP9 and Bone
 Brain Antibodies  >100 publications with MMP9 and Brain
 Liver Antibodies  >76 publications with MMP9 and Liver
 Bone Marrow Antibodies  >58 publications with MMP9 and Bone Marrow
 Kidney Antibodies  >54 publications with MMP9 and Kidney
 Connective Tissue Antibodies  >51 publications with MMP9 and Connective Tissue
 Embryonic Tissue Antibodies  >48 publications with MMP9 and Embryonic Tissue
 Lymph Node Antibodies  >43 publications with MMP9 and Lymph Node
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