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anti-S100A8 antibody :: Mouse Macrophage L1 Protein Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS439288
Immunohistochemistry (IHC)
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Macrophage L1 Protein (S100A8), Monoclonal Antibody

★Popular Item★
 Also Known As   

Macrophage L1 Protein Mouse Monoclonal Antibody

 Product Synonym Names    60B8AG; Calgranulin A (CAGA); Calgranulin B (CAGB); Calprotectin L1L subunit; Chemotactic cytokine CP-10; Cystic fibrosis antigen (CFAG); Leukocyte L1 complex light chain; L1Ag; Macrophage L1 protein Calprotectin; Migration inhibitory factor related protein 8; Myeloid-related protein 8 (MRP-8 or P8); Myeloid-related protein 14 (MRP-14 or P14); Neutrophil cytosolic 7kDa protein; NIF; p8; Migration inhibitory factor related protein 14; S100 calcium binding protein A8 (S100A8); S100 calcium binding protein A9 (S100A9); S100A8/S100A9 complex; MA387; Pro-inflammatory S100 cytokine; Urinary stone protein band A
 Product Gene Name   

anti-S100A8 antibody

[Similar Products]
 Product Synonym Gene Name    S100A9 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    123885
 3D Structure    ModBase 3D Structure for P05109
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 Clonality    Monoclonal
 Isotype    IgG1, kappa
 Clone Number    SPM281
 Host    Mouse
 Species Reactivity    Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Dog, Rabbit, Guinea Pig, Rat, Mouse
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 Specificity    Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Cellular Localization    Cytoplasmic
 Immunogen    Affinity purified monocyte membrane preparation
 Hu-Chromosome Location    1q21
 Positive Control    Tonsil, lymph node, or spleen
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 Preparation and Storage    Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of anti-S100A8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-S100A8 antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

 Application Notes for anti-S100A8 antibody    Known Applications & Suggested Dilutions:
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
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 Immunohistochemistry (IHC) of anti-S100A8 antibody    Formalin-fixed, paraffin-embedded human Tonsil stained with Macrophage L1 Protein Monoclonal Antibody (SPM281)
anti-S100A8 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for S100A8. It may not necessarily be applicable to this product.
 NCBI GI #    984290540
 NCBI GeneID    6279
 NCBI Accession #    NP_001306126.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001319197.1 [Other Products]
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 UniProt Primary Accession #    P05109 [Other Products]
 UniProt Secondary Accession #    Q5SY70; Q9UC84; Q9UC92; Q9UCJ0; Q9UCM6; A8K5L3; D3DV37 [Other Products]
 UniProt Related Accession #    P05109 [Other Products]
 Molecular Weight    12-14kDa (doublet)
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 NCBI Official Full Name    protein S100-A8 isoform b
 NCBI Official Synonym Full Names    S100 calcium binding protein A8
 NCBI Official Symbol    S100A8 [Similar Products]
 NCBI Official Synonym Symbols   
P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
[Similar Products]
 NCBI Protein Information    protein S100-A8
 UniProt Protein Name    Protein S100-A8
 UniProt Synonym Protein Names   
Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG
 Protein Family    Protein
 UniProt Gene Name    S100A8 [Similar Products]
 UniProt Synonym Gene Names    CAGA; CFAG; MRP8; CFAG; MRP-8; p8 [Similar Products]
 UniProt Entry Name    S10A8_HUMAN
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 NCBI Summary for S100A8    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
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 UniProt Comments for S100A8    S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoskeleton; cytosol; extracellular region; extracellular space; nucleus; plasma membrane

Molecular Function: arachidonic acid binding; calcium ion binding; microtubule binding; protein binding; RAGE receptor binding; zinc ion binding

Biological Process: activation of NF-kappaB transcription factor; autophagy; caspase activation; chemokine production; cytokine production; defense response to bacterium; defense response to fungus; inflammatory response; leukocyte migration during inflammatory response; neutrophil chemotaxis; peptidyl-cysteine S-nitrosylation; positive regulation of cell growth; positive regulation of inflammatory response; regulation of cytoskeleton organization and biogenesis; sequestering of zinc ion
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Product References and Citations for anti-S100A8 antibody

   Flavell DJ; Jones DB; Wright DH. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. Journal of Histochemistry and Cytochemistry, 1987, 35(11):1217-26.
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 Research Articles on S100A8    1. Treg cells suppress the activity of cytotoxic T cells, which kill malaria parasites; therefore, the up-regulation of S100A8 in malaria patients may contribute to pathogen immune escape or tolerance.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-S100A8 antibodyDiseases associated with anti-S100A8 antibody
 Reference Product  PubMed Publications
 S100A9 antibody  >894 publications with S100A8 and S100A9
 TLR4 antibody  >83 publications with S100A8 and TLR4
 MYD88 antibody  >13 publications with S100A8 and MYD88
 SRC antibody  >7 publications with S100A8 and SRC
 MIF antibody  >6 publications with S100A8 and MIF
 BAX antibody  >5 publications with S100A8 and BAX
 TIRAP antibody  >2 publications with S100A8 and TIRAP
 LY96 antibody  >2 publications with S100A8 and LY96
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >505 publications with S100A8 and Inflammation
 Disease Models, Animal Antibodies  >133 publications with S100A8 and Disease Models, Animal
 Fibrosis Antibodies  >112 publications with S100A8 and Fibrosis
 Necrosis Antibodies  >101 publications with S100A8 and Necrosis
 Cardiovascular Diseases Antibodies  >90 publications with S100A8 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >72 publications with S100A8 and Nervous System Diseases
 Atherosclerosis Antibodies  >51 publications with S100A8 and Atherosclerosis
 Brain Diseases Antibodies  >44 publications with S100A8 and Brain Diseases
 Heart Diseases Antibodies  >37 publications with S100A8 and Heart Diseases
 Kidney Diseases Antibodies  >35 publications with S100A8 and Kidney Diseases
Organs/Tissues associated with anti-S100A8 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >577 publications with S100A8 and Blood
 Skin Antibodies  >154 publications with S100A8 and Skin
 Lung Antibodies  >128 publications with S100A8 and Lung
 Vascular Antibodies  >103 publications with S100A8 and Vascular
 Bone Antibodies  >72 publications with S100A8 and Bone
 Brain Antibodies  >61 publications with S100A8 and Brain
 Bone Marrow Antibodies  >58 publications with S100A8 and Bone Marrow
 Kidney Antibodies  >56 publications with S100A8 and Kidney
 Liver Antibodies  >54 publications with S100A8 and Liver
 Heart Antibodies  >51 publications with S100A8 and Heart
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