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anti-NEP antibody :: Mouse anti-Human NEP / DDR1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS246492
Unit / Price
0.05 mL  /  $495 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NEP / DDR1, Monoclonal Antibody

 Also Known As   

Mouse Monoclonal [clone 2G4E12] (IgG1) to Human NEP / DDR1

 Product Synonym Names    Anti-NEP / DDR1 Antibody (clone 2G4E12) IHC-plus; DDR1; Cell adhesion kinase; CD167; CD167a antigen; EDDR1; ENTRK4; Mammary carcinoma kinase 10; MCK10; NEP; PTK3; PTK3A; RTK6; Tyrosine-protein kinase CAK; CAK; DDR; Discoidin domain receptor 1; HGK2; MCK-10; NTRK4; Protein-tyrosine kinase 3A; Human NEP; DDR1
 Product Gene Name   

anti-NEP antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    600408
 3D Structure    ModBase 3D Structure for Q08345
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    2G4E12
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Human DDR1
 Purity/Purification    Ascites
 Form/Format    Ascites fluid, 0.03% sodium azide. Sourced in Ascites.
 Concentration    1 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Purified recombinant fragment of DDR1(aa602-681) expressed in E Coli.
 Immunogen    NEP / DDR1 antibody was raised against purified recombinant fragment of DDR1(aa602-681) expressed in E Coli.
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 Preparation and Storage    Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
 Other Notes    Small volumes of anti-NEP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-NEP antibody

   DDR1 is a DDR/TKT type protein kinase. DDR1 is activated by various types of collagen, including types I through IV. Binding of collagen to DDR1 protein results in autophosphorylation and a delayed but sustained tyrosine kinase activation. DDR1 may function in cell-to-cell interaction or recognition. At least three mRNA variants, resulting in different protein isoforms of 875, 913 and 919 amino acids, have been reported.
 Product Categories/Family for anti-NEP antibody    Family: Protein Kinase
Subfamily: DDR/TKT
 Applications Tested/Suitable for anti-NEP antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-NEP antibody    ELISA (1:10000), IHC-P (1:200 - 1:300), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
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 Immunohistochemistry (IHC) of anti-NEP antibody    Anti-DDR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
anti-NEP antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-NEP antibody    Anti-DDR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
anti-NEP antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-NEP antibody    Western blot of anti-DDR1 monoclonal antibody against truncated MBP-DDR1 recombinant protein(1).
anti-NEP antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for NEP. It may not necessarily be applicable to this product.
 NCBI GI #    83977452
 NCBI GeneID    780
 NCBI Accession #    NP_054700.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013994.2 [Other Products]
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 UniProt Primary Accession #    Q08345 [Other Products]
 UniProt Secondary Accession #    Q14196; Q16562; Q2L6H3; Q4LE50; Q5ST11; Q5ST12; Q6NSK4; Q9UD35; B5A975; B5A976; B7Z2K0 [Other Products]
 UniProt Related Accession #    Q08345 [Other Products]
 Molecular Weight    99,064 Da
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 NCBI Official Full Name    epithelial discoidin domain-containing receptor 1 isoform 3
 NCBI Official Synonym Full Names    discoidin domain receptor tyrosine kinase 1
 NCBI Official Symbol    DDR1 [Similar Products]
 NCBI Official Synonym Symbols   
CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
[Similar Products]
 NCBI Protein Information    epithelial discoidin domain-containing receptor 1; tyrosine kinase DDR; cell adhesion kinase; mammary carcinoma kinase 10; tyrosine-protein kinase CAK; protein-tyrosine kinase RTK-6; neuroepithelial tyrosine kinase; PTK3A protein tyrosine kinase 3A; CD167 antigen-like family member A; neurotrophic tyrosine kinase, receptor, type 4
 UniProt Protein Name    Epithelial discoidin domain-containing receptor 1
 UniProt Synonym Protein Names   
CD167 antigen-like family member A; Cell adhesion kinase; Discoidin receptor tyrosine kinase; HGK2; Mammary carcinoma kinase 10; MCK-10; Protein-tyrosine kinase 3A; Protein-tyrosine kinase RTK-6; TRK E; Tyrosine kinase DDR; Tyrosine-protein kinase CAK; CD_antigen: CD167a
 Protein Family    Halolysin-like extracellular serine protease
 UniProt Gene Name    DDR1 [Similar Products]
 UniProt Synonym Gene Names    CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; Epithelial discoidin domain receptor 1; MCK-10 [Similar Products]
 UniProt Entry Name    DDR1_HUMAN
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 NCBI Summary for NEP    Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
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 UniProt Comments for NEP    DDR1: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2. Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells. Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; DDR family

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: collagen binding; protein binding; metal ion binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: lactation; negative regulation of cell proliferation; extracellular matrix organization and biogenesis; regulation of cell-matrix adhesion; protein amino acid autophosphorylation; smooth muscle cell migration; ear development; regulation of cell growth; cell adhesion; embryo implantation
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 Research Articles on NEP    1. In this review we highlight the mechanisms whereby DDRs(DDR1 and DDR2) regulate two important processes, namely inflammation and tissue fibrosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-NEP antibodyDiseases associated with anti-NEP antibody
 Products by Pathway  Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Non-integrin Membrane-ECM Interactions Pathway antibodies  Non-integrin Membrane-ECM Interactions Pathway Diagram
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >27 publications with NEP and Fibrosis
 Inflammation Antibodies  >14 publications with NEP and Inflammation
 Lung Neoplasms Antibodies  >14 publications with NEP and Lung Neoplasms
 Vascular Diseases Antibodies  >13 publications with NEP and Vascular Diseases
 Kidney Diseases Antibodies  >12 publications with NEP and Kidney Diseases
 Necrosis Antibodies  >12 publications with NEP and Necrosis
 Adenocarcinoma Antibodies  >10 publications with NEP and Adenocarcinoma
 Brain Diseases Antibodies  >9 publications with NEP and Brain Diseases
 Disease Models, Animal Antibodies  >9 publications with NEP and Disease Models, Animal
 Liver Diseases Antibodies  >7 publications with NEP and Liver Diseases
Organs/Tissues associated with anti-NEP antibody
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >42 publications with NEP and Lung
 Kidney Antibodies  >24 publications with NEP and Kidney
 Muscle Antibodies  >19 publications with NEP and Muscle
 Heart Antibodies  >19 publications with NEP and Heart
 Brain Antibodies  >19 publications with NEP and Brain
 Connective Tissue Antibodies  >13 publications with NEP and Connective Tissue
 Liver Antibodies  >13 publications with NEP and Liver
 Embryonic Tissue Antibodies  >10 publications with NEP and Embryonic Tissue
 Nerve Antibodies  >10 publications with NEP and Nerve
 Skin Antibodies  >9 publications with NEP and Skin
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