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NOS Clone 21C10-1D10 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS350037 anti-NOS Clone 21C10-1D10 antibody
Unit / Price
0.1 mL  /  $450 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NOS Clone 21C10-1D10, Monoclonal Antibody

★Popular Item★
 Also Known As   

Anti-human inducible Nitric Oxide Synthase Monoclonal Antibody 21C10-1D10

 Product Synonym Names    NOS Clone 21C10-1D10
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    Mouse IgG2b kappa
 Clone Number    21C10-1D10
 Species Reactivity    Human
Crossreactivity Note: No cross reaction with nNOS or eNOS Maps to hiNOS (25-54)
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 Specificity    Polypeptide ::: % Cross Reactivity
hiNOS (25-54) ::: 100
rhiNOS (Type II) ::: 100
hnNOS (201-231):::0
rhnNOS (Type I):::0
heNOS (25-54):::0
rheNOS (Type III):::0
 Form/Format    Supplied as Culture Supernatant
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 Preparation and Storage    Short term: Store at 2-8 degrees C.
Long term: Dilute the antibody with PBS that contains either 1.0% BSA or 1% normal goat serum, aliquoted into single use portions, and store frozen at -20 C: do not repeatedly freeze and thaw. The addition of 0.01% sodium azide or 0.01% thimerosal to inhibit microbial growth can be used to prolong the length of time the antibody the antibody can be stored in the refrigerator.
Note: 0.01% sodium azide or 0.01% thimerosal should only be used if they will not affect the utilization of the antibodies in your application. User are responsible for determining if these preservative agents can be used with their application.
 Other Notes    Small volumes of anti-NOS Clone 21C10-1D10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-NOS Clone 21C10-1D10 antibody

   This sterile filtered ascites fluid contains mouse monoclonal antibody clone 21C101D10 raised against recombinant hiNOS. It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153 amino acid length of hiNOS and was found to bind to a defined region of the hiNOS sequence, residues 25-54. This monoclonal antibody has been found to stain hiNOS in western immunoblots and by immunocytochemistry. This monoclonal antibody was tested for recognition of other NOS isoforms by ELISA, western immunoblotting, and immunocytochemical techniques. It has been found to be a mouse IgG2b kappa by isotyping.
 Product Categories/Family for anti-NOS Clone 21C10-1D10 antibody    Nitric Oxide Synthase Antibodies and Kits
 Applications Tested/Suitable for anti-NOS Clone 21C10-1D10 antibody   

Western Immunoblot, Western Blot, Immunofluorescent Staining of Induced Cells

 Application Notes for anti-NOS Clone 21C10-1D10 antibody    Western Blot: Western immunoblots resulted in a single band being detected at ~ 130 kDa at a dilution of 1:40

Immunofluorescent: This monoclonal antibody has been found to stain cells induced to produce iNOS at a 1:10 dilution. The ability of this monoclonal antibody to bind to iNOS in fixed cells was examined using three different cell lines. DLD-1 (a human colorectal epithelial cell line). A-172 (a human glioblastoma cell line). and RAW 264.7 (a mouse macrophage cell line). The cells were cultured. and then induced to produce iNOS. Following the induction. the cells were washed x 4 and fixed in either neutral buffered formalin or 100% ice cold. They were reacted for 60 minutes with the culture supernatant. and then with FITC-conjugated goat anti-mouse IgG. The staining pattern was observed using epifluorescent microscopy

Immunofluorescent: This monoclonal antibody has been found to stain cells induced to produce iNOS at a 1:10 dilution. The ability of this monoclonal antibody to bind to iNOS in fixed cells was examined using three different cell lines. DLD-1 (a human colorectal epithelial cell line). A-172 (a human glioblastoma cell line). and RAW 264.7 (a mouse macrophage cell line). The cells were cultured. and then induced to produce iNOS. Following the induction. the cells were washed x 4 and fixed in either neutral buffered formalin or 100% ice cold. They were reacted for 60 minutes with the culture supernatant. and then with FITC-conjugated goat anti-mouse IgG. The staining pattern was observed using epifluorescent microscopy

Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 7.5% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the mouse monoclonal antibody after preparing a 1:50 dilution. Use 2% normal goat serum in TBS/Tween-20 as buffer. and let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 min per wash in TBS/Tween-20 buffer.
7. Develop color using the enhanced DAB reaction.
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NCBI/Uniprot data below describe general gene information for NOS Clone 21C10-1D10. It may not necessarily be applicable to this product.
 NCBI GI #    295410244
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 NCBI Official Full Name    NOS
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-NOS Clone 21C10-1D10 antibodyPathways associated with anti-NOS Clone 21C10-1D10 antibody
 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with NOS Clone 21C10-1D10 and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with anti-NOS Clone 21C10-1D10 antibody
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with NOS Clone 21C10-1D10 and Bone
 Connective Tissue Antibodies  >4 publications with NOS Clone 21C10-1D10 and Connective Tissue
 Heart Antibodies  >3 publications with NOS Clone 21C10-1D10 and Heart
 Muscle Antibodies  >3 publications with NOS Clone 21C10-1D10 and Muscle
 Brain Antibodies  >3 publications with NOS Clone 21C10-1D10 and Brain
 Vascular Antibodies  >2 publications with NOS Clone 21C10-1D10 and Vascular
 Embryonic Tissue Antibodies  >2 publications with NOS Clone 21C10-1D10 and Embryonic Tissue
 Ear Antibodies  >1 publications with NOS Clone 21C10-1D10 and Ear
 Kidney Antibodies  >1 publications with NOS Clone 21C10-1D10 and Kidney
 Umbilical Cord Antibodies  >1 publications with NOS Clone 21C10-1D10 and Umbilical Cord
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