View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List NOS Clone 22E3-2F5 Monoclonal Antibody Catalog # MBS350039 Unit / Price 1 mL / $480 +1 FREE 8GB USB 5x1 mL / $2,045 +2 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name NOS Clone 22E3-2F5, Monoclonal Antibody Full Product Name Anti-human inducible Nitric Oxide Synthase Monoclonal Antibody 22E3-2F5 Product Synonym Names NOS Clone 22E3-2F5 Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype Mouse IgG1 kappa Clone Number 22E3-2F5 Species Reactivity HumanCrossreactivity Note: No cross reaction with nNOS or eNOS Maps to hiNOS (781-798) Specificity Polypeptide ::: % Cross ReactivityhiNOS (781-798) ::: 100rhiNOS (Type II) ::: ~85 Form/Format Supplied as Culture Supernatant Other Notes Small volumes of anti-NOS Clone 22E3-2F5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-NOS Clone 22E3-2F5 antibody This culture supernatant contains mouse monoclonal antibody clone 22E3-2F5 raised against recombinant hiNOS. It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153 amino acid length of hiNOS and was found to bind to a defined region of the hiNOS sequence. residues 781-798. This monoclonal antibody has been found to stain hiNOS in western immunoblots and by immunocytochemistry. This monoclonal antibody was tested for recognition of other NOS isoforms by ELISA. western immunoblotting. and immunocytochemical techniques. It has been found to be a mouse IgG1 kappa by isotyping Product Categories/Family for anti-NOS Clone 22E3-2F5 antibody Nitric Oxide Synthase Antibodies and Kits Applications Tested/Suitable for anti-NOS Clone 22E3-2F5 antibody Western Immunoblot, Western Blot, Immunofluorescent Staining of Induced Cells Application Notes for anti-NOS Clone 22E3-2F5 antibody Western Blot: Western immunoblots resulted in a single band being detected at ~ 130 kDa at a dilution of 1:40Immunofluorescent: This monoclonal antibody has been found to stain cells induced to produce iNOS at a 1:80 dilution. The ability of this monoclonal antibody to bind to iNOS in fixed cells was examined using three different cell lines. DLD-1 (a human colorectal epithelial cell line). A-172 (a human glioblastoma cell line). and RAW 264.7 (a mouse macrophage cell line). The cells were cultured. and then induced to produce iNOS. Following the induction. the cells were washed x 4 and fixed in either neutral buffered formalin or 3% freshly prepared formaldehyde. They were reacted for 60 minutes with the culture supernatant. and then with FITC-conjugated goat anti-mouse IgG. The staining pattern was observed using epifluorescent microscopyImmunofluorescent: This monoclonal antibody has been found to stain cells induced to produce iNOS at a 1:80 dilution. The ability of this monoclonal antibody to bind to iNOS in fixed cells was examined using three different cell lines. DLD-1 (a human colorectal epithelial cell line). A-172 (a human glioblastoma cell line). and RAW 264.7 (a mouse macrophage cell line). The cells were cultured. and then induced to produce iNOS. Following the induction. the cells were washed x 4 and fixed in either neutral buffered formalin or 3% freshly prepared formaldehyde. They were reacted for 60 minutes with the culture supernatant. and then with FITC-conjugated goat anti-mouse IgG. The staining pattern was observed using epifluorescent microscopyWestern Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 7.5% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.2. Wash x 2 with TBS/Tween-20.3. Apply the mouse monoclonal antibody after preparing a 1:40 dilution. Use 2% normal goat serum in TBS/Tween-20 as buffer. and let the primary antibody bind for 2-4 hours.4. Wash x 3 with TBS/Tween-20.5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.6. Wash x 4 for 5 min per wash in TBS/Tween-20 buffer.7. Develop color using the enhanced DAB reaction TOP NCBI/Uniprot data below describe general gene information for NOS Clone 22E3-2F5. It may not necessarily be applicable to this product. NCBI GI # 295410244 TOP NCBI Official Full Name NOS TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with anti-NOS Clone 22E3-2F5 antibody Reference Product PubMed Publications FLNA antibody >12 publications with NOS Clone 22E3-2F5 and FLNA Pathways associated with anti-NOS Clone 22E3-2F5 antibody Products by Pathway Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Organs/Tissues associated with anti-NOS Clone 22E3-2F5 antibody Organ/Tissue Name Pubmed Publications Bone Antibodies >13 publications with NOS Clone 22E3-2F5 and Bone Connective Tissue Antibodies >4 publications with NOS Clone 22E3-2F5 and Connective Tissue Heart Antibodies >3 publications with NOS Clone 22E3-2F5 and Heart Muscle Antibodies >3 publications with NOS Clone 22E3-2F5 and Muscle Brain Antibodies >3 publications with NOS Clone 22E3-2F5 and Brain Vascular Antibodies >2 publications with NOS Clone 22E3-2F5 and Vascular Embryonic Tissue Antibodies >2 publications with NOS Clone 22E3-2F5 and Embryonic Tissue Ear Antibodies >1 publications with NOS Clone 22E3-2F5 and Ear Kidney Antibodies >1 publications with NOS Clone 22E3-2F5 and Kidney Umbilical Cord Antibodies >1 publications with NOS Clone 22E3-2F5 and Umbilical Cord TOP