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anti-NOTCH 1 antibody :: Mouse NOTCH 1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS212783
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NOTCH 1, Monoclonal Antibody

 Also Known As   

MOUSE ANTI MOUSE NOTCH 1

 Product Gene Name   

anti-NOTCH 1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q01705
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    mN1A
 Host    Mouse
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 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
Preparation
 Buffer Solution    Phosphate buffered saline
 Target Species    Mouse
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-NOTCH 1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-NOTCH 1 antibody

   Mouse anti Mouse Notch 1 antibody, clone mN1A specifically recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoeisis, myelopoiesis, and neurogenesis.
 Applications Tested/Suitable for anti-NOTCH 1 antibody   

Immunohistology Frozen, Flow cytometry (FC/FACS)*, Western Blot (WB)

 Application Notes for anti-NOTCH 1 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
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 Testing Data of anti-NOTCH 1 antibody    Staining of mouse thymocytes with Mouse anti Mouse NOTCH 1:Alexa Fluor 488 (MBS212782A488)
anti-NOTCH 1 antibody Testing Data image
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 Testing Data of anti-NOTCH 1 antibody    Staining of mouse thymocytes with Mouse anti Mouse NOTCH-1:Alexa Fluor 647 (MBS212782A647)
anti-NOTCH 1 antibody Testing Data image
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 Testing Data of anti-NOTCH 1 antibody    Staining of mouse thymocytes with Mouse anti Mouse NOTCH-1:FITC (MBS219495)
anti-NOTCH 1 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for NOTCH 1. It may not necessarily be applicable to this product.
 NCBI GI #    224967065
 NCBI GeneID    18128
 NCBI Accession #    NP_032740.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_008714.3 [Other Products]
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 UniProt Primary Accession #    Q01705 [Other Products]
 UniProt Secondary Accession #    Q06007; Q3TZW2; Q3U3Y2; Q61905; Q7TQ50; Q7TQ51; Q7TQ52; Q8K428; Q99JC2; Q9QW58; Q9R0X7 [Other Products]
 UniProt Related Accession #    Q01705 [Other Products]
 Molecular Weight    269,180 Da
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 NCBI Official Full Name    neurogenic locus notch homolog protein 1
 NCBI Official Synonym Full Names    notch 1
 NCBI Official Symbol    Notch1 [Similar Products]
 NCBI Official Synonym Symbols   
N1; Mis6; Tan1; lin-12; 9930111A19Rik
[Similar Products]
 NCBI Protein Information    neurogenic locus notch homolog protein 1; Motch A; Notch gene homolog 1; mT14; major type A protein; p300; transmembrane receptor Notch1
 UniProt Protein Name    Neurogenic locus notch homolog protein 1
 UniProt Synonym Protein Names   
Motch A; mT14; p300Cleaved into the following 2 chains:Notch 1 extracellular truncation; NEXT; Notch 1 intracellular domain; NICD
 UniProt Gene Name    Notch1 [Similar Products]
 UniProt Synonym Gene Names    Motch; Notch 1; NEXT; NICD [Similar Products]
 UniProt Entry Name    NOTC1_MOUSE
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 UniProt Comments for NOTCH 1    Notch 1: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Oncoprotein; Transcription factor; Membrane protein, integral

Cellular Component: Golgi apparatus; cell surface; membrane; cell; integral to plasma membrane; cytoplasm; plasma membrane; integral to membrane; acrosome; nucleus; receptor complex

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; sequence-specific DNA binding; chromatin DNA binding; metal ion binding; chromatin binding; calcium ion binding; receptor activity; transcription factor activity

Biological Process: neural tube development; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; heart development; positive regulation of JAK-STAT cascade; positive regulation of glial cell differentiation; regulation of cell migration; negative regulation of BMP signaling pathway; neuron differentiation; compartment specification; positive regulation of endothelial cell differentiation; negative regulation of ossification; somatic stem cell division; negative regulation of osteoblast differentiation; embryonic limb morphogenesis; positive regulation of astrocyte differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of keratinocyte differentiation; negative regulation of photoreceptor cell differentiation; positive regulation of neuroblast proliferation; cell fate commitment; transcription, DNA-dependent; neuron fate commitment; regulation of transcription from RNA polymerase II promoter; keratinocyte differentiation; negative regulation of neuron differentiation; branching morphogenesis of a tube; regulation of gene expression; response to muramyl dipeptide; positive regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; negative regulation of transcription, DNA-dependent; anagen; foregut morphogenesis; positive regulation of epithelial cell proliferation; osteoblast fate commitment; endoderm development; transcription from RNA polymerase II promoter; epidermis development; cell fate specification; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; regulation of epithelial cell proliferation; negative regulation of neurogenesis; negative regulation of cell proliferation; regulation of neurogenesis; glial cell differentiation; astrocyte differentiation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; forebrain development; cardiac muscle morphogensis; angiogenesis; heart looping; regulation of somitogenesis; cell differentiation; positive regulation of BMP signaling pathway; regulation of developmental process; mesenchymal cell development; Notch signaling pathway; hair follicle morphogenesis; in utero embryonic development; lumen formation; liver development; humoral immune response; activation of Notch receptor target transcription factor; regulation of cell proliferation; negative regulation of oligodendrocyte differentiation; negative regulation of cell differentiation; inflammatory response to antigenic stimulus; axonogenesis; negative regulation of catalytic activity; epithelial to mesenchymal transition; spermatogenesis; sprouting angiogenesis; negative regulation of myoblast differentiation; auditory receptor cell fate commitment; lung development; positive regulation of cell migration
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 Research Articles on NOTCH 1    1. ADAM10 plays an essential role in the activation of Notch-1 signaling and has a remarkable effect on neuronal maintenance in adult mouse brain
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-NOTCH 1 antibodyDiseases associated with anti-NOTCH 1 antibody
 Products by Pathway  Pathway Diagram
 Activated NOTCH1 Transmits Signal To The Nucleus Pathway antibodies  Activated NOTCH1 Transmits Signal To The Nucleus Pathway Diagram
 Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway antibodies  Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Disease Name  Pubmed Publications
 Lymphoma Antibodies  >430 publications with NOTCH 1 and Lymphoma
 Nervous System Diseases Antibodies  >221 publications with NOTCH 1 and Nervous System Diseases
 Precursor T-Cell Lymphoblastic Leukemia-Lymphoma Antibodies  >209 publications with NOTCH 1 and Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
 Adenocarcinoma Antibodies  >177 publications with NOTCH 1 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >158 publications with NOTCH 1 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >119 publications with NOTCH 1 and Breast Neoplasms
 Congenital Abnormalities Antibodies  >97 publications with NOTCH 1 and Congenital Abnormalities
 Inflammation Antibodies  >83 publications with NOTCH 1 and Inflammation
 Liver Diseases Antibodies  >82 publications with NOTCH 1 and Liver Diseases
 Lung Neoplasms Antibodies  >78 publications with NOTCH 1 and Lung Neoplasms
Organs/Tissues associated with anti-NOTCH 1 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >671 publications with NOTCH 1 and Blood
 Bone Antibodies  >365 publications with NOTCH 1 and Bone
 Brain Antibodies  >351 publications with NOTCH 1 and Brain
 Embryonic Tissue Antibodies  >303 publications with NOTCH 1 and Embryonic Tissue
 Bone Marrow Antibodies  >237 publications with NOTCH 1 and Bone Marrow
 Muscle Antibodies  >228 publications with NOTCH 1 and Muscle
 Heart Antibodies  >166 publications with NOTCH 1 and Heart
 Thymus Antibodies  >161 publications with NOTCH 1 and Thymus
 Liver Antibodies  >144 publications with NOTCH 1 and Liver
 Skin Antibodies  >138 publications with NOTCH 1 and Skin
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