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anti-NPM1 antibody :: Mouse anti-Human NPM1 / NPM / Nucleophosmin Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS245427
Unit / Price
0.05 mg  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NPM1 / NPM / Nucleophosmin, Monoclonal Antibody

 Also Known As   

Mouse Monoclonal [clone 5E3] (IgG2b,k) to Human NPM1 / NPM / Nucleophosmin

 Product Synonym Names    Anti-NPM1 / NPM / Nucleophosmin Antibody (aa81-294, clone 5E3) IHC-plus; NPM1; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; B23; Nucleophosmin; Human NPM1; NPM; Nucleophosmin
 Product Gene Name   

anti-NPM1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    164040
 3D Structure    ModBase 3D Structure for P06748
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 Clonality    Monoclonal
 Isotype    IgG2b,k
 Clone Number    5E3
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    Protein G Purified
 Form/Format    PBS, pH 7.4, 0.1% sodium azide
 Concentration    1 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Recombinant human NPM (81-294aa) purified from E Coli.
 Immunogen Type    Recombinant protein
 Immunogen    NPM1 / NPM / Nucleophosmin antibody was raised against recombinant human NPM (81-294aa) purified from E Coli.
 Antigen Modification    aa81-294
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 Preparation and Storage    Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
 Other Notes    Small volumes of anti-NPM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-NPM1 antibody

   NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.
 Applications Tested/Suitable for anti-NPM1 antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-NPM1 antibody    ELISA, IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
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 Immunohistochemistry (IHC) of anti-NPM1 antibody    Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-NPM1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-NPM1 antibody    Anti-NPM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-NPM1 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-NPM1 antibody    The HeLa and HepG2 cell lysates (5 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-NPM1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for NPM1. It may not necessarily be applicable to this product.
 NCBI GI #    10835063
 NCBI GeneID    4869
 NCBI Accession #    NP_002511.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002520.6 [Other Products]
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 UniProt Primary Accession #    P06748 [Other Products]
 UniProt Secondary Accession #    P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6 [Other Products]
 UniProt Related Accession #    P06748 [Other Products]
 Molecular Weight    28,400 Da
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 NCBI Official Full Name    nucleophosmin isoform 1
 NCBI Official Synonym Full Names    nucleophosmin (nucleolar phosphoprotein B23, numatrin)
 NCBI Official Symbol    NPM1 [Similar Products]
 NCBI Official Synonym Symbols   
B23; NPM
[Similar Products]
 NCBI Protein Information    nucleophosmin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1
 UniProt Protein Name    Nucleophosmin
 UniProt Synonym Protein Names   
Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin
 Protein Family    Nucleophosmin
 UniProt Gene Name    NPM1 [Similar Products]
 UniProt Synonym Gene Names    NPM; NPM [Similar Products]
 UniProt Entry Name    NPM_HUMAN
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 NCBI Summary for NPM1    This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
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 UniProt Comments for NPM1    NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: Oncoprotein; Translation; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; nucleus; cytosol; ribonucleoprotein complex

Molecular Function: protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal large subunit binding; NF-kappaB binding; protein binding; Tat protein binding; protein heterodimerization activity; ribosomal small subunit binding

Biological Process: regulation of centriole replication; viral reproduction; positive regulation of translation; centrosome cycle; cell aging; nucleocytoplasmic transport; signal transduction; DNA repair; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; nucleosome assembly; negative regulation of cell proliferation; protein localization; regulation of endodeoxyribonuclease activity; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis
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 Research Articles on NPM1    1. Mutations in FLT3, NPM1, and CEBPA provide In this review, prognostic information and have been integrated into clinical diagnostics and clinical practice for acute myeloid leukemia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-NPM1 antibodyDiseases associated with anti-NPM1 antibody
 Products by Pathway  Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 BARD1 Signaling Events Pathway antibodies  BARD1 Signaling Events Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway antibodies  Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 Host Interactions Of HIV Factors Pathway antibodies  Host Interactions Of HIV Factors Pathway Diagram
 Interactions Of Rev With Host Cellular Proteins Pathway antibodies  Interactions Of Rev With Host Cellular Proteins Pathway Diagram
 Disease Name  Pubmed Publications
 Leukemia, Myeloid, Acute Antibodies  >639 publications with NPM1 and Leukemia, Myeloid, Acute
 Cell Transformation, Neoplastic Antibodies  >33 publications with NPM1 and Cell Transformation, Neoplastic
 Leukemia, Promyelocytic, Acute Antibodies  >23 publications with NPM1 and Leukemia, Promyelocytic, Acute
 Carcinoma Antibodies  >22 publications with NPM1 and Carcinoma
 Anemia Antibodies  >14 publications with NPM1 and Anemia
 Adenocarcinoma Antibodies  >10 publications with NPM1 and Adenocarcinoma
 Disease Models, Animal Antibodies  >9 publications with NPM1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >9 publications with NPM1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >7 publications with NPM1 and Neoplasms, Experimental
 Neoplasm Metastasis Antibodies  >5 publications with NPM1 and Neoplasm Metastasis
Organs/Tissues associated with anti-NPM1 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >231 publications with NPM1 and Blood
 Bone Antibodies  >148 publications with NPM1 and Bone
 Bone Marrow Antibodies  >146 publications with NPM1 and Bone Marrow
 Lung Antibodies  >9 publications with NPM1 and Lung
 Brain Antibodies  >7 publications with NPM1 and Brain
 Skin Antibodies  >5 publications with NPM1 and Skin
 Prostate Antibodies  >4 publications with NPM1 and Prostate
 Spleen Antibodies  >4 publications with NPM1 and Spleen
 Heart Antibodies  >4 publications with NPM1 and Heart
 Liver Antibodies  >4 publications with NPM1 and Liver
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