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anti-NPHS1 antibody :: Rabbit anti-Human Nephrin Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS684285
Flow Cytometry (FC/FACS)
Unit / Price
0.1 mL  /  $210 +1 FREE 8GB USB
100 Tests  /  $915 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Nephrin (NPHS1), Monoclonal Antibody

★Popular Item★
 Also Known As   

Anti-Nephrin FITC

 Product Synonym Names    Anti-nephrin
 Product Gene Name   

anti-NPHS1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    256300
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    D13-E
 Host    Rabbit
 Species Reactivity    Human
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 Form/Format    Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
 Concentration    0.2 mg/mL clonal IgGs (lot specific)
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 Preparation    The antibody was prepared by in vitro cloning technology, and the monospecific product was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
 Important Notes    This antibody detects intracellular antigen (cells of interest must be permeabilized).
Optimal dilution should be determined by the end user.
Use 1106 cells in a 100 ul experimental sample (a test).
This product is for in vitro use only, research or diagnostic applications.
 Caution    Product contains sodium azide.
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 Preparation and Storage    Store undiluted at 4C, protect from prolonged exposure to light. Do not freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Other Notes    Small volumes of anti-NPHS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-NPHS1 antibody

   FITC Rabbit Anti-Human Nephrin
 Applications Tested/Suitable for anti-NPHS1 antibody   

This antibody detects intracellular antigen( cells of interest must be permeabilized).

Optimal dilutions should be determined by the end user.

 Application Notes for anti-NPHS1 antibody    Use 1 x 10 6 cells in a 100 uL experimental sample (a test).
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 Flow Cytometry (FC/FACS) of anti-NPHS1 antibody    Profile of nephrin expression in human Hek293 cell line analyzed.

Cells were fixed, permeabilized and stained with anti-Nephrin FITC (green, used 10 ul per test) or with an isotype control (black).
anti-NPHS1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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NCBI/Uniprot data below describe general gene information for NPHS1. It may not necessarily be applicable to this product.
 NCBI GI #    10441644
 NCBI GeneID    4868
 NCBI Accession #    AAG17141.1 [Other Products]
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 UniProt Secondary Accession #    A6NDH2; C3RX61 [Other Products]
 UniProt Related Accession #    O60500 [Other Products]
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 NCBI Official Full Name    nephrin
 NCBI Official Synonym Full Names    NPHS1 nephrin
 NCBI Official Symbol    NPHS1 [Similar Products]
 NCBI Official Synonym Symbols   
CNF; NPHN; nephrin
[Similar Products]
 NCBI Protein Information    nephrin
 UniProt Protein Name    Nephrin
 UniProt Synonym Protein Names   
Renal glomerulus-specific cell adhesion receptor
 Protein Family    Nephrin
 UniProt Gene Name    NPHS1 [Similar Products]
 UniProt Synonym Gene Names    NPHN [Similar Products]
 UniProt Entry Name    NPHN_HUMAN
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 NCBI Summary for NPHS1    This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]
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 UniProt Comments for NPHS1    NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell projection; integral to plasma membrane; intracellular; plasma membrane

Molecular Function: myosin binding; protein binding

Biological Process: cell adhesion; excretion; glomerular basement membrane development; JNK cascade; myoblast fusion; positive regulation of actin filament polymerization; skeletal muscle development

Disease: Nephrotic Syndrome, Type 1
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 Research Articles on NPHS1    1. Activated IQGAP1, as an intracellular partner of nephrin, is involved in actin cytoskeleton organization and functional regulation of podocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-NPHS1 antibodyPathways associated with anti-NPHS1 antibody
 Reference Product  PubMed Publications
 NPHS2 antibody  >86 publications with NPHS1 and NPHS2
 WT1 antibody  >48 publications with NPHS1 and WT1
 CD2AP antibody  >25 publications with NPHS1 and CD2AP
 TRPC6 antibody  >11 publications with NPHS1 and TRPC6
 TJP1 antibody  >6 publications with NPHS1 and TJP1
 SRC antibody  >4 publications with NPHS1 and SRC
 KIRREL antibody  >4 publications with NPHS1 and KIRREL
 FYN antibody  >1 publications with NPHS1 and FYN
 Products by Pathway  Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Nephrin Interactions Pathway antibodies  Nephrin Interactions Pathway Diagram
 Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway antibodies  Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway Diagram
Diseases associated with anti-NPHS1 antibodyOrgans/Tissues associated with anti-NPHS1 antibody
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >158 publications with NPHS1 and Kidney Diseases
 Nephrosis Antibodies  >121 publications with NPHS1 and Nephrosis
 Proteinuria Antibodies  >83 publications with NPHS1 and Proteinuria
 Glomerulonephritis Antibodies  >41 publications with NPHS1 and Glomerulonephritis
 Glomerulosclerosis, Focal Segmental Antibodies  >41 publications with NPHS1 and Glomerulosclerosis, Focal Segmental
 Nephritis Antibodies  >40 publications with NPHS1 and Nephritis
 Kidney Failure, Chronic Antibodies  >18 publications with NPHS1 and Kidney Failure, Chronic
 Renal Insufficiency Antibodies  >18 publications with NPHS1 and Renal Insufficiency
 Nephrosis, congenital Antibodies  >13 publications with NPHS1 and Nephrosis, congenital
 Disease Models, Animal Antibodies  >13 publications with NPHS1 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >141 publications with NPHS1 and Kidney
 Pancreas Antibodies  >8 publications with NPHS1 and Pancreas
 Brain Antibodies  >6 publications with NPHS1 and Brain
 Thymus Antibodies  >3 publications with NPHS1 and Thymus
 Testis Antibodies  >2 publications with NPHS1 and Testis
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